Anti—α1-Antichymotrypsin Staining of 194 Sarcomas, 38 Carcinomas, and 17 Malignant Melanomas Its Lack of Specificity as a Tumour Marker

Leader, M.; Patel, J.; Collins, M.; Henry, Kristin

doi:10.1097/00000478-198702000-00007

Cited by 78 publications

(18 citation statements)

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“…However, AAT and AACT lack specificity as markers for malignant histiocytes because they have been found in T-cell lymphomas and several types of nonhematopoietic malignancies. 18,20,36,37 Immunoreactivity of neoplastic histiocytes for immunoglobulins is consistent with documented human malignancies that are histiocytic in origin. 20 The utility oflectins (SBA, PNA, and ConA) as probes for identifying mononuclear phagocytes and their malignant derivatives has been investigated previously.…”

Section: Discussionsupporting

confidence: 71%

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

et al. 1993

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Diagnosis of malignant histiocytosis (MH), a disorder characterized by systemic proliferation of morphologically atypical histiocytes and their precursors, in an 8-year-old neutered female Golden Retriever was based on light and electron microscopic and immunohistochemical findings. Clinically, the dog presented with unilateral forelimb lameness. Eight days after surgical exploration of a swollen brachium, the dog developed sudden onset of posterior paresis, fecal and urinary incontinence, and a flaccid tail. Necropsy revealed infiltrative and nodular lesions in the right forelimb and regional lymph nodes, thoracic and abdominal cavities, and lumbar epidural space. Gross lesions were not found in the lungs or integument. Histopathologic examination showed infiltrates of atypical histiocytes in skeletal muscle, joint, and regional lymph nodes of the right forelimb; intercostal muscle; lung; liver; spleen; pancreas; kidneys; and spinal dura. Most tumor infiltrates were nodular and composed of loosely aggregated cells that were 10-30 μm in diameter with abundant eosinophilic to foamy cytoplasm, had central or eccentric nuclei, and were periodic acid-Schiff negative. Many binucleated cells, multinucleated giant cells, and mitotic figures were seen. Tumor cells contained phagocytosed erythrocytes, mononuclear cells, and some leukocytes. Ultrastructural features of tumor cells included cytoplasmic lipid droplets, lysosomes, and phagolysosomes. Immunohistochemical studies on paraffin-embedded sections showed positive reactivity to human T-cell Ag (clone UCHL-1) and for lysozyme, α-1-antitrypsin, and cathespin B. Polyclonal intracellular immunoglobulin reactivity and lectin binding (peanut, soybean, and wheat germ agglutinins and concanavalin A) were also demonstrated. Criteria for diagnosis of malignant histiocytic tumors and differential diagnosis are discussed.

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Section: Discussionsupporting

confidence: 71%

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

et al. 1993

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“…Thus, although positivity with cdACT antiserum has been presumed to be demonstrative of a histiocytic origin of MFH cells, cdACT positivity was found in many different cells or tissues. Recent results and a review of the literature [25] reinforces the lack of specificity of ~IACT in the diagnosis of the histiocytic nature of tumors. Even more non-specific were the results obtained with NSE and $100 antiserum, as had been already suggested [26][27][28].…”

Section: Discussionmentioning

confidence: 85%

Primary breast sarcoma: A review of 33 cases with immunohistochemistry and prognostic factors

Terrier

Terrier-Lacombe

Mouriesse

et al. 1989

Breast Cancer Res Tr

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The clinical and pathological features of 33 previously untreated patients with primary breast sarcoma were retrospectively analysed to evaluate the prognostic significance of histologic variables on survival. The series comprised 17 cystosarcomas phyllodes and 16 stromal sarcomas (excluding angiosarcomas). All tumors were reviewed and classified in similar fashion to extramammary soft tissue sarcomas. In addition, immunohistochemical studies were performed on paraffin sections with a panel of several antibodies directed against cytoskeletal filaments and cellular enzymes; five cases were also examined by electron microscopy. Most tumors were malignant fibrous histiocytoma (21 cases) and fibrosarcoma (6 cases) types. Surgery was the main therapy. Metastasis-free survival rate was significantly correlated only with histological grade, consisting of tumor differentiation, tumor necrosis, and mitotic activity. Courses and survivals of the cystosarcoma and stromal groups were identical, questioning the clinical value of this pathologic distinction. All local recurrence, metastasis, or death occurred within 30 months, though follow-up was much longer. Immunohistochemistry was disappointing for identification of specific histologic sub-types.

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“…A hitiocytic marker, CD68, exhibited weakly positive immunoreactivity in giant cells in the three cases cases. Regardless of the nomenclature of "MFH", histiocytic markers (CD68, α1-antitrypsin, α1-antichymotrypsin, lysozyme, and factor XIII) no longer have useful roles in the diagnosis of MFHs (Leader et al 1987;Cassidy et al 1994;Christopher et al 2002;Morris et al 2002;Szollosi et al 2005). To summarise, "vimentin only" immunoreactivity without any other specific or distinct expression of cell line markers can indicate a diagnosis of MFH.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic approach to malignant fibrous histiocytomas of soft tissue in dogs: a case report

Ko¹,

Kim²,

Choi³

et al. 2013

Vet. Med.

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Malignant fibrous histiocytomas (MFHs), newly named as 'undifferentiated pleomorphic sarcomas' in 2002 by the World Health Organization, generally show an ambiguous origin. They have been described to fibroblastic or histiocytic in origin, while storiform-pleomorphic variants share a similar morphologic pattern with other sarcomas. For this reason additional diagnostic methods including immunology, ultrastructure analysis and molecular approaches are necessary for a more accurate diagnosis. We report three cases of MFH in individual dogs which presented histological characteristics of pleomorphic sarcomatoid tumours. Microscopic investigation, immunohistochemistry (vimentin, CD68, desmin, α-smooth muscle actin, and S-100) and electron microscopy were performed for diagnosis. One case was diagnosed as storiform-pleomorphic type and two were diagnosed as giant cell type MFHs. The present study demonstrates concepts of MFHs based on newly described categories and suggests useful diagnostic approaches for uncertain sarcomatoid soft tissue tumours in canines.

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Anti—α1-Antichymotrypsin Staining of 194 Sarcomas, 38 Carcinomas, and 17 Malignant Melanomas Its Lack of Specificity as a Tumour Marker

Cited by 78 publications

References 0 publications

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

Primary breast sarcoma: A review of 33 cases with immunohistochemistry and prognostic factors

Diagnostic approach to malignant fibrous histiocytomas of soft tissue in dogs: a case report

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