Anti-Zika virus activity and chemical characterization by ultra-high performance liquid chromatography (UPLC-DAD-UV-MS) of ethanol extracts in Tecoma species

Abstract

Background Plant species from the genus Tecoma are found in tropical and subtropical regions around the world. Some of them are grown as ornamental plants and others can be used as medicinal plants. In the present study, ethanolic extracts from trunks and leaves of Tecoma species were tested in vitro using assays against the Zika virus. Methods There was a total of 8 extracts obtained from different anatomical parts of three Tecoma species. The Tecoma castaneifolia , T. garrocha , T. stans var. angustata and T. stans var. stans were prepared by percolation with ethanol. The antiviral activity was assayed in vitro against the Zika virus by the MTT colorimetric method ( n = 3). The UPLC-DAD-MS analysis of ethanolic extracts was performed from all the studied species. The biofractionation of T. stans var. stans trunk extract using different separation techniques led to the isolation of crenatoside compound. Results Ethanolic extract from Tecoma species leaves were more active against the Zika virus (EC 50 149.90 to 61.25 μg/mL) when compared to the trunk extracts tested (EC 50 131.0 to 66.79 μg/mL and two were not active). The ethyl acetate and aqueous fractions obtained from T. stans var. stans trunk were active against the Zika virus with EC 50 values of 149.90 and 78.98 μg/mL, respectively. Crenatoside is a phenylethanoid glycoside isolated from the ethyl acetate of T. stans var. stans trunk extract. This compound was tested and exhibited EC 50 34.78 μM (21.64 μg/mL), thus demonstrating a better result than the original ethanolic extracts as well as others extracts of Tecoma species, and it was more active than the positive control, ribavirin (386.84 μM). Furthermore, its selectivity index was at least 2.5 times higher than the tested ethanolic extracts and 11.1 times more potent than ribavirin. Conclusion The Tecoma species demonstrated interesting in vitro activity against the Zika virus . The crenatoside, phenylethanoid glycoside that was for the first time isolated from Tecoma stans var. stans , exhibited a potent and relevant anti- Zika virus activity, being more active than ribavirin (positive ...

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

“…quinquefasciatus (Hari & Mathew, 2018). In another axis, ethanolic extract, ethyl acetate, and aqueous fractions from Tecoma species leaves, exhibited potent in vitro antiviral activity against the Zika virus (Reis et al, 2020). Ovicidal activity and oviposition deterrence efficiency of aqueous leaf extracts of T. stans were reported on Ae.…”

“…To assess T. stans extracts as safer and effective mosquitocides, several studies were undertaken on its repellent potential (Gupta & Gupta, 2020). Recently, Reis et al (2020) demonstrated its potential against an infectious mosquito‐borne flavivirus (Zika). Previously, some T. stans extracts were tested on mosquitoes, such as the effectiveness of petroleum ether extract on Ae.…”

Antiviral Activity of Reagents in Mouth Rinses against SARS-CoV-2

“…Citrox, which is derived from citrus fruits, is composed of soluble bioflavonoids and hydroxylated phenolic structures produced by plants. Bioflavonoids have demonstrated their capacity to act against bacteria, fungi, and viruses ( Hooper et al 2011 ; Lalani et al 2020 ; Reis et al 2020 ; Zou et al 2020 ).…”

Bruguiera gymnorhiza (L.) Lam. at the Forefront of Pharma to Confront Zika Virus and Microbial Infections—An In Vitro and In Silico Perspective

“…The Ministry of Health in Brazil has reported 579 new ZIKV cases between December 2019 and February 2020 [ 9 , 10 ]. First identified in Uganda in 1947 from rhesus monkeys, the ZIKV, particularly vectored by the yellow fever mosquito, Aedes aegypti , is a flavivirus originating from the Flaviviridae family [ 11 ]. The virus can be transmitted sexually, via blood transfusions, via organ transportation and from pregnant women to their fetuses [ 12 , 13 ].…”

“…Crenatoside identified from Tecoma stans var. stans displayed better anti-ZIKV activity (EC50: 34.78 µM) than the positive control ribavirin (EC50: 386.84 µM) [ 11 ]. Lipophilic ethers from citrus fruits present the ability to selectively inhibit ZIKV replication in human breast carcinoma (4T1) cells [ 17 ].…”