Anti-Worm Antibody Specificities in 129/J Mice Resistant to Infection with Schistosoma japonicum

Mitchell, Graham F.; Cruise, Kathy M; Garcia, E. G.; Tiu, Wilfred U.

doi:10.2307/3281655

Cited by 17 publications

(14 citation statements)

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“…In view of the potential relationship between the antibody response of WEHI 129/J mice to Sj26 and their genetic resistance to S.japonicum infection (2,3), the cDNA present in one of the Sj26 cDNA clones (phage X clone 117) (5) was subcloned into pUR292 (generating the plasmid pSjl), and its entire nucleotide sequence was deduced (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

“…Whereas all mice of a susceptible strain such as BALB/c become infected following challenge with 25 S. japonicum cercariae, =50% of exposed WEHI 129/J mice are resistant in that they contain no adult worms (1)(2)(3). Exposed WEHI 129/J mice possess serum antibody specificities that are different from those found in sera from infected BALB/c mice, and in particular, are high responders to a S. japonicum adult worm antigen of Mr 26,000 (Sj26) (2,3).…”

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confidence: 99%

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Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

Smith¹,

Davern²,

Board³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

202

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authors request that the following correction be noted. Recent nucleotide sequence analysis has established that a C residue was omitted at position 628 of the Sj26 cDNA sequence presented in Fig. 2. The amended nucleotide sequence and the altered prediction of the COOH-terminal structure of Sj26 are given below. This correction does not otherwise affect the conclusions of the paper. ABSTRACT Mice of the inbred strain 129/J bred at this Institute (WEHI 129/J) are relatively resistant to chronic infection with the parasitic helminth Schistosomajaponicum. In contrast to more permissive mouse strains such as BALB/c, the WEHI 129/J mice are high responders to a Mr 26,000 adult worm antigen designated Sj26. Cloned cDNAs corresponding to Sj26 have been identified in a S. japonicum phage Xgtll amp3 expression library, and their nucleotide sequences have been deduced. The predicted amino acid sequence of the antigen specified by these cDNAs shows striking homology with class it isozymes of mammalian glutathione S-transferases (RX:glutathione R-transferase, EC 2.5.1.18). Extracts of adult worms contain glutathione S-transferase activity, and affinity chromatography of enzyme activity on glutathione columns leads to the purification of a Mr 26,000 molecule that comigrates with Sj26. Although vaccination studies in mice with a .3-galactosidase-Sj26 fusion protein from Escherichia coli are encouraging, more immunogenic preparations of the antigen are likely to be required to establish the utility of Sj26 as a model vaccine.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

Smith¹,

Davern²,

Board³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

202

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“…Worms were recovered by portal perfusion and dissection of the liver and lungs (Mitchell et al, 1981). Sera were also obtained from i29/J mice that remained free of infection after six percutaneous challenges with 5. japonicum (Philippines) cercariae (Mitchell et al, 1984(Mitchell et al, , 1985.…”

Section: Serum Collection and Parasite Recoverymentioning

confidence: 99%

“…Recent studies have identified a mouse strain, 129/J, that is relatively resistant to infection with the trematode parasite Schistosoma japonicum (Philippines) (Garcia, Tiu and Mitchell, 1983b;Mitchell et al, 1984Mitchell et al, , 1985. A large proportion of adult mice exposed once or repeatedly to cercariae contain no adult worms in the portal system 4-5 weeks later.…”

Section: Introductionmentioning

confidence: 99%

RESISTANCE OF 129/J MICE TO SCHISTOSOMA MANSONI INFECTION

A²,

Jc³

et al. 1986

Aust J Exp Biol Med

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Summary. Mice of the strain 129/J bred at this Institute (WEHI 129/J) were exposed once or repeatedly to cercariae of Schistosoma mansoni and worm burdens determined. In keeping with reports of S. japonicum infection in these mice, a high level of resistance was recorded. Radio-labelled extracted antigens of S. mansoni adult worms were reacted with sera from exposed 129/J mice and infected BALB/c mice. Immunoprecipitated antigens preferentially recognized by antibodies in 129/J mice include proteins of Mr 99,000 and 21,000. Whether responses to these antigens are a cause or a consequence of resistance remains to be established.

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“…In the search for a vaccine against initial and persistent infection with the trematode parasite Schistosoma japonicum, an abundant Mr 26,000 protein (Sj26) that is a GST has emerged as one contender (1)(2)(3)(4). This antigen is recognized particularly well in immune Abbreviations used in this paper: ABA, azobenzenearsonate; AWE, adult worm extract in phosphate-buffered saline (PBS); ELISA, enzyme-linked immunosorbent assay; FCA, Freund's complete adjuvant; GSH, reduced glutathione; GST, glutathione S-transferase from aduh worm extracts, eluted from GSH-agarose (i.e.…”

Section: Introductionmentioning

confidence: 99%

Responses in mice to Sj26, a glutathione S‐transferase of Schistosoma japonicum worms

Morahan

et al. 1987

Immunol Cell Biol

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Summary The genetic variation in antibody responses of mice to glutathione S‐transferase (GST) enzymes of Schistosoma japonicum worms, and in particular to a Mr 26,000 species termed Sj26, was analysed. Sera from infected mice, or mice immunized with adjuvant and an Sj26 β‐galactosidase fusion protein produced in Escherichia coli (Sj26FP), or purified near‐native recombinant Sj26 produced in E. coli (rSj26), were assayed by enzyme‐linked immunosorbent assay (ELISA) for antibody titres to GST purified from adult worms. Anti‐GST antibody levels are high in a mouse strain, WEHI 129/J, that is genetically resistant to infection with S. japonicum. Antibody responses to GST are low in BALB/c mice and intermediate in most other mouse strains analysed such as CBA/H and C57B1/6. Responsiveness to Sj26 in adjuvant is dominant in (BALB/c × WEHI 129/J)F1 hybrids. BALB/c.H‐2b and BALB/c.H‐2k mice are higher responders than BALB/c. One feature of antibody responses to Sj26 is the variability within a group of mice. When rSj26 conjugated to the hapten azobenzenearsonate was used as immunogen, BALB/c mice produced substantial amounts of anti‐Sj26 antibodies. In an attempt to correlate antibody levels with resistance in infected mice, a new functional assay was devised to measure the ability of sera to inhibit the binding of rSj26 to glutathione. However, there was no correlation between inhibitory titre in this assay and the numbers of worms recovered. In regard to the candidacy of GST as a vaccinating antigen in schistosomiasis japonica, the data raise the problem of variable responsiveness to the antigen that will need to be overcome by antigen modification and/or powerful adjuvants.

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Anti-Worm Antibody Specificities in 129/J Mice Resistant to Infection with Schistosoma japonicum

Cited by 17 publications

References 0 publications

Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

RESISTANCE OF 129/J MICE TO SCHISTOSOMA MANSONI INFECTION

Responses in mice to Sj26, a glutathione S‐transferase of Schistosoma japonicum worms

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