Anti-Vibrio dibutyl phthalate from marine-derived Streptomyces sp. S073

Bi, Yunwen; Liu, Ganxing; Yu, Qin; Liang, Qiting; Xu, Zhongheng; Cui, Miao; Zhang, Qizhong; Xu, Dapeng

doi:10.1016/j.rvsc.2021.09.001

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…The Actinomycete isolates were determined to be within the genus Streptomyces using amplification of the 16S rRNA gene (Supplementary Table S1). The genus is distinguished by the production of a wide range of extracellular enzymes and bioactive secondary metabolites with diverse structural and functional properties that are used as antibacterial, antifungal, antiviral, antioxidant, immunomodulatory, anticancer, antibiofilm agents 12 , and anti-vibrio compounds [31][32][33][34] . Streptomyces labedae, for example, has been shown to inhibit the biofilm-forming ability of Acinetobacter and Moraxella 35 , and S. variabilis has an antibiofilm effect against the human clinical pathogens V. cholerae, Escherichia coli, and Staphylococcus aureus 36 .…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Miller

Waturangi²,

Yogiara³

2022

Sci Rep

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In nature, bacteria can form biofilms, multi-layered structures that adhere microbial populations to solid surfaces by exopolysaccharides, proteins, and nucleic acids. In addition to causing foodborne infections, biofilms can be a major problem in aquaculture. Actinomycetes extracts have previously demonstrated antibiofilm activity against multiple foodborne and fish pathogens, and further characterization of these extracts is needed. In this study, we identified the chemical structures and antibiofilm properties of four extracts and determined the genetic similarity of the isolates to known Streptomyces isolates. We found that several extracts contained multiple antibiofilm compounds, and the antibiofilm activities of all extracts were most stable at pH 6. Furthermore, the antibiofilm inhibition and destruction activities of the isolates were stable at different temperatures. All of crude extracts demonstrated activity against biofilms formed by foodborne and fish pathogens on the surface of stainless-steel coupons as well as polystyrene that commonly used in industrial equipment. Using PCR 16S-rRNA gene and DNA sequencing analysis, the four Actinomycetes isolates were found to be 99% (1 AC), 97% (20 PM), 95% (16 PM), and 85% (18 PM) similar to Streptomyces. Biofilm structure were analyzed using Scanning Electron Microscopy coupled with Energy-Dispersive Spectrometry analysis. Coniine/(S)-2-propylpiperidine was the most active fraction of the crude extracts of the 1 AC, 20 PM, and 16 PM isolates, and piperidine, 2-(tetrahydro-2-furanyl) was most active in the 18 PM isolate.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Miller

Waturangi²,

Yogiara³

2022

Sci Rep

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“…Research has shown the various potential mechanisms behind the anti-Vibrio properties of Streptomyces sp [92][93][94][95] . For instance, the production of siderophores or enzymes by streptomycetes has been shown to inhibit the growth of Vibrios in aquatic environments [96,97] .…”

Section: Data Descriptionmentioning

confidence: 99%

Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium that is ubiquitous in marine environments. Its perilous co-existence with aquatic animals increases the risk of infections and diseases, especially those in aquaculture systems, thus resulting in reduced production and economic losses in the aquaculture industry. Moreover, V. parahaemolyticus can be easily transmitted to humans via consumption of contaminated seafood, resulting in gastroenteritis outbreaks. However, the rise in multidrug resistance within the species has challenged the efficacy of antimicrobial treatments against V. parahaemolyticus infections. Therefore, we report the genome sequence of V. parahaemolyticus RP0132 isolated from shrimp to gain insight into its antimicrobial resistance traits and potential resistance mechanisms. The findings will facilitate the development of effective anti-Vibrio agents to manage V. parahaemolyticus infections.

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“…In a follow-up study with the same strain of Streptomyces sp., the researchers identified dibutyl phthalate (DBP) as another compound that elicits inhibitory effects against V. parahaemolyticus. It was concluded that the antagonistic effects of S073 were dependent on the synergistic effects of DBP-mediated antagonism and siderophore-governed iron competition with V. parahaemolyticus [146] . The vibriocidal activity of Streptomyces sp.…”

Section: Harnessing the Anti-vibrio Properties Of Streptomyces Spmentioning

confidence: 99%

Vibrio parahaemolyticus: Exploring its Incidence in Malaysia and the Potential of Streptomyces sp. as an Anti-Vibrio Agent

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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As the world recovers from the COVID-19 pandemic, concern remains for future potential outbreaks because of the persisting effects of climate change, including the proliferation of infectious diseases. The frequent isolation of Vibrio parahaemolyticus in the surrounding environment is of concern as it can cause infections in marine animals and transmitted to humans. V. parahaemolyticus is the leading cause of foodborne gastroenteritis worldwide. Malaysia is one of the top seafood consumers and this places us at a higher risk of exposure to V. parahaemolyticus infections. Over the years, this foodborne pathogen has been isolated from various sources in Malaysia, mainly from seafood such as shellfish, shrimps, and fish. To make matters worse, there has been an emergence of antibiotic-resistant V. parahaemolyticus worldwide, which is attributed to the uncontrolled use of antibiotics in aquaculture to prevent and treat vibriosis. Therefore, it is vital to utilize alternatives such as probiotics to control V. parahaemolyticus to prevent further propagation of antibiotic-resistant strains of the bacteria. A potential candidate for probiotics is Streptomyces sp., a class of filamentous, Gram-positive bacteria that produce a variety of bioactive compounds during their life cycle, which can be useful in drug discovery. The bioactive compounds produced by Streptomyces sp. have been proven to have microbiota-modulating and stimulatory effects on the host, enhancing immunity and providing protective effects against V. parahaemolyticus infections. With the application of Streptomyces sp. as probiotics in aquaculture, the efficacy of the available antibiotics can be preserved, and the further spread of antibiotic resistance in the environment can be reduced.

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Anti-Vibrio dibutyl phthalate from marine-derived Streptomyces sp. S073

Cited by 7 publications

References 30 publications

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Vibrio parahaemolyticus: Exploring its Incidence in Malaysia and the Potential of Streptomyces sp. as an Anti-Vibrio Agent

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