Anti-Vascular Endothelial Growth Factor (VEGF) Antibody Ameliorates Cartilage Degradation in a Rat Model of Chronic Sports Arthritic Injury

Shang, Lei; Xu, Yong; Shao, Changqing; Ma, Chao; Yuan, Feng

doi:10.12659/msm.906954

Cited by 4 publications

(5 citation statements)

References 27 publications

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“…Inflammation during early-stage OA is induced by cytokines, especially TNF-α and IL-1β [31]. In consistence with recent results [32], the present study showed that the levels of TNF-α and IL-1β were elevated in the knee synovial fluid of model rats. However, miR-30b-5p down-regulation decreased the levels of IL-1β and TNF-α in model rats.…”

Section: Discussionsupporting

confidence: 90%

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MiR-30b-5p Influences Chronic Exercise Arthritic Injury by Targeting Hoxa1

Gai

Chen

2021

Int J Sports Med

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We identified the role of miR-30b-5p in chronic exercise arthritic injury. Rats with chronic exercise arthritic injury received treatment with miR-30b-5p antagomiR. H&E and Safranin O-fast green staining were performed. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected. The binding relationship between homeobox A1 (Hoxa1) and miR-30b-5p was revealed. After manipulating the expressions of miR-30b-5p and/or Hoxa1 in chondrocytes, the viability, apoptosis and migration of chondrocytes were assessed. The levels of molecules were determined by qRT-PCR or Western blot. MiR-30b-5p antagomiR ameliorated articular cartilage lesion and destruction, reduced Mankin’s score and the levels of TNF-α, IL-1β, miR-30b-5p, matrix metallopeptidase 13 (MMP-13), and cleaved caspase-3, and increased relative thickness and the levels of Hoxa1, Aggrecan and type II collagen (COLII) in model rats. MiR-30b-5p up-regulation decreased Hoxa1 level, viability, migration and induced apoptosis, whereas miR-30b-5p down-regulation produced the opposite effects. MiR-30b-5p up-regulation increased the levels of MMP-13 and cleaved caspase-3, but decreased those of Aggrecan and COLII in chondrocytes. However, the action of miR-30b-5p up-regulation on chondrocytes was reversed by Hoxa1 overexpression. In conclusion, miR-30b-5p is involved in cartilage degradation in rats with chronic exercise arthritic injury and regulates chondrocyte apoptosis and migration by targeting Hoxa1.

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Section: Discussionsupporting

confidence: 90%

“…Inflammation during early-stage OA is induced by cytokines, especially TNF-α and IL-1β [31]. In consistence with recent results [32], the present study showed that the levels of TNF-α and IL-1β were elevated in the knee synovial fluid of This document was downloaded for personal use only. Unauthorized distribution is strictly prohibited.…”

Section: Discussionsupporting

confidence: 88%

MiR-30b-5p Influences Chronic Exercise Arthritic Injury by Targeting Hoxa1

Gai

Chen

2021

Int J Sports Med

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“…In a traumatic brain injury model, mice treated with Bevacizumab had a significant drop in TNF-α levels, highlighting the ability of Bevacizumab to reduce inflammatory processes and subsequent vascular complications [27]. This anti-inflammatory feature of Bevacizumab was also confirmed in rats subjected to chronic sports arthritic injuries by reducing the levels of TNF-α [28].…”

Section: Effect Of Bevacizumab On Tnf-αmentioning

confidence: 76%

Targeting VEGF using Bevacizumab attenuates sepsis-induced liver injury in a mouse model of cecal ligation and puncture

Hadi,

Zaini,

Jawad

2023

JMedLife

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Sepsis, a life-threatening condition resulting from an uncontrolled host response to infection, often leads to severe liver damage and remains a significant cause of mortality in critically ill patients despite advances in antibiotic therapy and resuscitation. Bevacizumab, a neutralizing antibody targeting vascular endothelial growth factor (VEGF), is approved for treating certain cancers. However, its potential impact on sepsis-related liver injury is not well understood. This study aimed to explore the potential hepatoprotective effect of Bevacizumab on sepsis-induced liver injury. Twenty-four mice were divided into four groups: a sham group subjected to a midline incision only, a cecal ligation and puncture induction (CLP) group, a vehicle-treated group that received a vehicle one hour before CLP induction, and a Bevacizumab-treated group that received Bevacizumab one hour before CLP induction. Blood samples were collected, and angiopoietin-2 (ANGPT2), alanine transaminase (ALT), and aspartate transaminase (AST) serum levels were measured. Liver tissue homogenates were analyzed for IL-6, TNFα, intracellular adhesion molecule (ICAM-1), macrophage inhibitory factor (MIF), vascular endothelial growth factor (VEGF), F2-isoprostane, and caspase-11 levels. A histological examination was performed to assess the extent of liver damage. Mice exposed to CLP had high levels of the biomarkers mentioned above with a high degree of liver injury compared to the sham group. In contrast, treatment with Bevacizumab notably reduced these markers and mitigated liver damage. In conclusion, Bevacizumab may be a protective agent against sepsis-induced liver injury.

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“…It is generally believed that there is a close relationship between angiogenesis and osteogenesis [11,[23][24][25][26]. In the process of articular cartilage degeneration, hypertrophic chondrocytes enable microvessels to grow into the growth Journal of Nanomaterials plate by secreting angiogenic factors, such as VEGF, which leads to cartilage absorption and bone formation [27,28]. During bone repair, microvessels provide a prerequisite for the exchange of oxygen and nutrients for bone regeneration [23].…”

Section: Discussionmentioning

confidence: 99%

Repair of Osteoporotic Bone Defects Using Adipose-Derived Stromal Cells and Umbilical Vein Endothelial Cells Seeded in Chitosan/Nanohydroxyapatite-P24 Nanocomposite Scaffolds

Fang

Gong

Yang

et al. 2021

Journal of Nanomaterials

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Background. The cell regeneration and blood supply of bone defect lesions are restricted under osteoporotic pathological conditions, which make the healing of bone defect of osteoporosis still a great challenge. The current therapeutic strategies that mainly inhibit bone resorption are not always satisfactory for osteoporotic bone defects, which make the development of new therapies an urgent need. Methods. Previously, we prepared chitosan/nanohydroxyapatite (CS/nHA) biomimetic nanocomposite scaffolds for controlled delivery of bone morphogenetic protein 2-derived peptide (P24). In this study, we determined the effect of coculturing adipose-derived stromal cells (ADSCs) and human umbilical vein endothelial cells (HUVECs) with the CS-P24/nHA nanocomposite scaffolds on osteoporotic bone defect healing. In vitro mixed coculture models were employed to assess the direct effects of coculture. Results. ADSCs cocultured with HUVECs showed significantly greater osteogenic differentiation and mineralization compared with ADSCs or HUVECs alone. The CS-P24/nHA scaffold cocultured with ADSCs and HUVECs was more effective in inducing osteoporotic bone repair, as demonstrated by micro-computed tomography and histology of critical-sized calvariae defects in ovariectomized rats. Calvariae defects treated with the CS-P24/nHA nanocomposite scaffold plus ADSC/HUVEC coculture had a greater area of repair and better reconstitution of osseous structures compared with defects treated with the scaffold plus ADSCs or the scaffold plus HUVECs after 4 and 8 weeks. Conclusion. Taken together, coculture of ADSCs and HUVECs with the CS-P24/nHA nanocomposite scaffold is an effective combination to repair osteoporotic bone defects.

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Anti-Vascular Endothelial Growth Factor (VEGF) Antibody Ameliorates Cartilage Degradation in a Rat Model of Chronic Sports Arthritic Injury

Cited by 4 publications

References 27 publications

MiR-30b-5p Influences Chronic Exercise Arthritic Injury by Targeting Hoxa1

MiR-30b-5p Influences Chronic Exercise Arthritic Injury by Targeting Hoxa1

Targeting VEGF using Bevacizumab attenuates sepsis-induced liver injury in a mouse model of cecal ligation and puncture

Repair of Osteoporotic Bone Defects Using Adipose-Derived Stromal Cells and Umbilical Vein Endothelial Cells Seeded in Chitosan/Nanohydroxyapatite-P24 Nanocomposite Scaffolds

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