Anti-V antigen antibody protects macrophages from Yersinia pestis -induced cell death and promotes phagocytosis

Weeks, S.; Hill, Jim; Friedlander, Arthur M.; Welkos, Susan L.

doi:10.1006/mpat.2002.0498

Cited by 75 publications

(110 citation statements)

References 51 publications

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“…To test the immunogenicity of the Salmonella-F1-Ag vaccine, mice orally immunized with 2 ϫ 10 9 CFU showed, by week 4, elevated serum IgG endpoint titers of 2 17 and copro-IgA Ab titers of 2 10 Fig. 2A.…”

Section: Oral Immunization With Salmonella-f1-ag Elicits Elevated Sysmentioning

confidence: 99%

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Oral Vaccination withSalmonellaSimultaneously ExpressingYersinia pestisF1 and V Antigens Protects against Bubonic and Pneumonic Plague

Yang

Hinnebusch

Trunkle

et al. 2007

The Journal of Immunology

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The gut provides a large area for immunization enabling the development of mucosal and systemic Ab responses. To test whether the protective Ags to Yersinia pestis can be orally delivered, the Y. pestis caf1 operon, encoding the F1-Ag and virulence Ag (V-Ag) were cloned into attenuated Salmonella vaccine vectors. F1-Ag expression was controlled under a promoter from the caf1 operon; two different promoters (P), PtetA in pV3, PphoP in pV4, as well as a chimera of the two in pV55 were tested. F1-Ag was amply expressed; the chimera in the pV55 showed the best V-Ag expression. Oral immunization with Salmonella-F1 elicited elevated secretory (S)-IgA and serum IgG titers, and Salmonella-V-Ag(pV55) elicited much greater S-IgA and serum IgG Ab titers than Salmonella-V-Ag(pV3) or Salmonella-V-Ag(pV4). Hence, a new Salmonella vaccine, Salmonella-(F1+V)Ags, made with a single plasmid containing the caf1 operon and the chimeric promoter for V-Ag allowed the simultaneous expression of F1 capsule and V-Ag. Salmonella-(F1+V)Ags elicited elevated Ab titers similar to their monotypic derivatives. For bubonic plague, mice dosed with Salmonella-(F1+V)Ags and Salmonella-F1-Ag showed similar efficacy (>83% survival) against ∼1000 LD50 Y. pestis. For pneumonic plague, immunized mice required immunity to both F1- and V-Ags because the mice vaccinated with Salmonella-(F1+V)Ags protected against 100 LD50 Y. pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis.

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Section: Oral Immunization With Salmonella-f1-ag Elicits Elevated Sysmentioning

confidence: 99%

“…In addition, it has been suggested that V-Ag can act as an immunosuppressive agent, alter host cytokine production (15), and inhibit neutrophil chemotaxis (16). Based on these observations, perhaps the protective effect of anti-V-Ag Abs is due to their ability to neutralize V-Aginduced immunosuppression (17).…”

mentioning

confidence: 99%

Oral Vaccination withSalmonellaSimultaneously ExpressingYersinia pestisF1 and V Antigens Protects against Bubonic and Pneumonic Plague

Yang

Hinnebusch

Trunkle

et al. 2007

The Journal of Immunology

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“…P rogrammed cell death or apoptosis plays a major regulatory role in immune responses (1). TNF-␣ released early in response to bacterial infections (2) and, specifically, several bacterial pathogens can directly trigger apoptosis in cells of the monocyte/macrophage lineage (3)(4)(5)(6)(7)(8)(9)(10). However, the molecular mechanisms of apoptosis induced by bacteria are incompletely delineated, particularly regarding the involvement of death receptors.…”

Section: Two Distinct Mechanisms For Induction Of Dendritic Cell Apopmentioning

confidence: 99%

“…Bacteria-pulsed BMDCs and non-pulsed BMDCs were collected at different time points, washed, and adjusted to 10 6 BMDCs/ml in cold 10 mM HEPES/NaOH (pH 7.4) containing 140 mM NaCl, 2.5 mM CaCl 2 , and 5 g/ml PI and Annexin V-FITC (BD PharMingen). BMDCs were incubated for 15 min at room temperature in the dark and were immediately analyzed by flow cytometry.…”

Section: Annexin V-fitc Stainingmentioning

confidence: 99%

Two Distinct Mechanisms For Induction of Dendritic Cell Apoptosis in Response to IntactStreptococcus pneumoniae

Colino

Snapper

2003

The Journal of Immunology

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Apoptotic dendritic cells (DCs) are ineffective at inducing immunity. Thus, parameters that regulate DC viability during a primary infection will help to determine the outcome of the subsequent immune response. In this regard, pathogens have developed strategies to promote DC apoptosis to counterbalance the nascent primary immune response. We demonstrate, using cultured bone marrow-derived DCs, that Streptococcus pneumoniae can induce DC apoptosis through two distinct mechanisms: 1) a rapid, caspase-independent mechanism of apoptosis induction, critically dependent on bacterial expression of pneumolysin, and 2) a delayed-onset, caspase-dependent mechanism of apoptosis induction associated with terminal DC maturation. Delayed-onset apoptosis does not require bacterial internalization, but rather is triggered by the interaction of bacterial subcapsular components and bone marrow-derived DC (likely Toll-like) receptors acting in a myeloid differentiation factor 88-dependent manner. In this regard, heavy polysaccharide encapsulation interferes with both DC maturation and apoptosis induction. In contrast, neither CD95/CD95 ligand interactions nor TNF-α appear to play a role in the delayed onset of apoptosis. These data are the first to define two mechanistically distinct pathways of DC apoptosis induction in response to an extracellular bacterium that likely have important consequences for the establishment of antibacterial immunity.

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“…From here, LcrV promotes assembly of the translocon pore in the eukaryotic cell plasma membrane (6,15,27). Since antibodies specific for LcrV also prevent effector translocation and protect animals from challenge with virulent yersiniae (8,18,32,62), LcrV is being pursued as a vaccine candidate against plague and other Yersinia infections (59). LcrV also impacts the positive regulatory loop needed for Yop synthesis.…”

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confidence: 99%

Diminished LcrV Secretion Attenuates Yersinia pseudotuberculosis Virulence

2007

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Anti-V antigen antibody protects macrophages from Yersinia pestis -induced cell death and promotes phagocytosis

Cited by 75 publications

References 51 publications

Oral Vaccination withSalmonellaSimultaneously ExpressingYersinia pestisF1 and V Antigens Protects against Bubonic and Pneumonic Plague

Oral Vaccination withSalmonellaSimultaneously ExpressingYersinia pestisF1 and V Antigens Protects against Bubonic and Pneumonic Plague

Two Distinct Mechanisms For Induction of Dendritic Cell Apoptosis in Response to IntactStreptococcus pneumoniae

Diminished LcrV Secretion Attenuates Yersinia pseudotuberculosis Virulence

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