Anti-urolithiatic activity of Bryophyllum pinnatum Lam. hydroalcoholic extract in sodium oxalate-induced urolithiasis in rats

Pandhare, Ramdas; Shende, R.R.; Avhad, M.S.; Deshmukh, V. K.; Mohite, Popat; Sangameswaran, B.; Daude, R.B.

doi:10.1016/j.jtcme.2021.06.002

Cited by 15 publications

(36 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total of 30 Wistar rats aged 6-8 weeks weighing (150-200 g) were divided into five groups, with six animals in each group (n=6), in the following manner [1]:…”

Section: Experimental Methodsmentioning

confidence: 99%

“…• Group 1: Normal control -Vehicle, that is, Normal Saline (10 ml/kg, p.o.) [1] for 28 days. • Group 2: Disease control -Sodium oxalate (75 mg/kg, i.p) [1] for 28 days.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The medicinal plant plays an important role in the traditional system. Herbal medicine is the formulation in which plants are used for formulation [1].…”

Section: Introductionmentioning

confidence: 99%

“…It provides safe and well-tolerated remedies for chronic illness. Since many existing synthetic drugs cause various side effects [1,2].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pharmacological Evaluation of Antiurolithiatic Activity of Dolichos Biflorus Seedextract in Rat’s Model

HAQUE

AL-SHAMI

CHATTERJEE

2022

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: The main aim of the present study was to investigate the antiurolithiatic activity of Dolichos Biflorus methanolic seed extract in a rat’s model. In the phytochemical screening, it was found that Dolichos Biflorus seed extract showed the presence of tannins steroids, protein, flavonoids, terpenoids, mucilage, saponin, and carbohydrate and the absence of alkaloids fixed oil. Hence, this plant has highly diuretic activity. Methods: Model: Sodium oxalate induced urolithiasis in rats. A total of 30 rats were used for this study and the animals were divided into five groups. Each group contains six rats: Normal control group, disease control group (sodium oxalate 75 mg/kg, IP), standard group (Cystone, 750 mg/kg, PO), treatment group1 (Dolichos Biflorus 150 mg/kg, PO), treatment Group 2 (Dolichos Biflorus 300 mg/kg, PO). Urolithiasis was induced by using sodium oxalate (75 mg/kg, IP) for 28 days. Results: At the end of the experiment, all the animal blood samples were collected to check the various biochemical parameters. Animals were sacrificed by giving a high dose of pentobarbitone and kidneys were collected for antioxidant and histopathological study. From the renal function test, it was found that the drug is showing a potent effect when compared to the disease control group and standard group. Moreover, from the antioxidant and histopathology study, it was found that the drug is showing a potent effect when compared to the disease control and standard group and control group. Conclusion: After all the investigation, it was found that oral administration of Dolichos Biflorus seed extract at the low dose of 150 mg/kg and the high dose of 300 mg/kg against the sodium oxalate-induced urolithiasis and it was found that high is more effective as compared to low dose. Drug was able to suppress oxalate synthesizing enzymes and minerals. Moreover, histopathology study in the treatment group showed recovery and normal architecture of glomerulus with a tuft of capillaries surrounded by Bowman’s capsule. The most of tubules are showing normal architecture and recovery. After seeing all the results, it is confirmed that the test drug Dolichos Biflorus has potent antiurolithiatic activity. (Dolichos Biflorus short form mentioned below as DB)

show abstract

“…Total of 30 Wistar rats aged 6-8 weeks weighing (150-200 g) were divided into five groups, with six animals in each group (n=6), in the following manner [1]:…”

Section: Experimental Methodsmentioning

confidence: 99%

“…• Group 1: Normal control -Vehicle, that is, Normal Saline (10 ml/kg, p.o.) [1] for 28 days. • Group 2: Disease control -Sodium oxalate (75 mg/kg, i.p) [1] for 28 days.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The medicinal plant plays an important role in the traditional system. Herbal medicine is the formulation in which plants are used for formulation [1].…”

Section: Introductionmentioning

confidence: 99%

“…It provides safe and well-tolerated remedies for chronic illness. Since many existing synthetic drugs cause various side effects [1,2].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pharmacological Evaluation of Antiurolithiatic Activity of Dolichos Biflorus Seedextract in Rat’s Model

HAQUE

AL-SHAMI

CHATTERJEE

2022

Asian J Pharm Clin Res

View full text Add to dashboard Cite

show abstract

“…Calcium, BUN (Blood Urea Nitrogen) uric acid, creatinine, potassium, alanine immune transferase, Phosphorus, oxalate, Alkaline Phosphatase [13,14]. The euthanized rats were conducted through a high dose of Pentobarbital followed by scarification.…”

Section: Observed Parametersmentioning

confidence: 99%

Evaluation of Anti Urolithiatic Activity of Dolichos biflorus Seed Extract by Using Ethylene Glycol Induced Model

Haque¹,

Al-Shami²,

Chatterjee³

2022

JPRI

View full text Add to dashboard Cite

In the phytochemical screening, it was found that Dolichos Biflorus seed extract contained the presence of tannins steroids, protein, flavonoids, terpenoids, mucilage, saponin, and carbohydrate, but no alkaloids fixed oil. So, this plant has highly diuretic activity. Which can use for the treatment of urolithiasis. Objective: The Present study aims to investigate the anti-Urolithiatic activity of Dolichos Biflorus methanolic seed extract in a rat model. Methods: Dolichos Biflorus Seeds Extract were evaluated for anti-Urolithiatic activity. Urolithiasis has been induced in Wistar rats through Ethylene glycol (0.75%v/v, p.o). Upon completion of the experiment, animals in all groups have been sacrificed and biochemical parameters such as; calcium, creatinine, Phosphorus, Uric acid, Alkaline Phosphatase BUN (Blood Urea Nitrogen), and histology of Kidney have been observed. Furthermore, Potassium, Oxalate levels, and Alanine Amino Transferases were measured. Results: The extract of Dolichos Biflorus was proven to be safe in the toxicity findings. It has been shown that in-vivo significant effect of plant extract was able to manage the urolithiatic markers such as calcium, creatinine, phosphorus, uric acid, alkaline phosphatase, BUN (Blood Urea Nitrogen), and potassium. In the urolithiatic rats it has been observed that abundant crystal depositions, renal epithelial cells had more tubular dilatation and damage shown by large spaces in the tissue, renal stone deposition damages the renal tissue and detoriate the renal function. Urolithiatic markers such as calcium, creatinine, phosphorus, uric acid, alkaline phosphatase, BUN (Blood Urea Nitrogen), and potassium were normal in animals receiving plant extract and prevent renal cell injury. Conclusion: All its findings and phytoconstituents existing inside the extract must stay the viable chemical materials involved in the prevention of Urolithiatic.

show abstract

Syrupy herbal formulation of green bean pod extract of Phaseolus vulgaris L.: Formulation optimization by central composite design, and evaluation for anti-urolithiatic activity

Noorulla,

Doyo Dalecha,

Jemal Haji

et al. 2024

Heliyon

View full text Add to dashboard Cite

Anti-urolithiatic activity of Bryophyllum pinnatum Lam. hydroalcoholic extract in sodium oxalate-induced urolithiasis in rats

Cited by 15 publications

References 36 publications

Pharmacological Evaluation of Antiurolithiatic Activity of Dolichos Biflorus Seedextract in Rat’s Model

Pharmacological Evaluation of Antiurolithiatic Activity of Dolichos Biflorus Seedextract in Rat’s Model

Evaluation of Anti Urolithiatic Activity of Dolichos biflorus Seed Extract by Using Ethylene Glycol Induced Model

Syrupy herbal formulation of green bean pod extract of Phaseolus vulgaris L.: Formulation optimization by central composite design, and evaluation for anti-urolithiatic activity

Contact Info

Product

Resources

About