Anti-ulcer Effects of Lactic Acid Bacteria and Their Cell Wall Polysaccharides.

Nagaoka, Masato; Hashimoto, Shusuke; Watanabe, Tsunekazu; Yokokura, Teruo; Mori, Yo

doi:10.1248/bpb.17.1012

Cited by 104 publications

(66 citation statements)

References 2 publications

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“…These results suggest that a metabolite(s) of these bacteria plays an important role in the prevention of ileal ulcer formation. Nagaoka et al (25) reported that lactic acid bacteria and their cell wall polysaccharides were effective for healing gastric ulcers induced by acetic acid. However, in our study, cells of L. acidophilus and B. adolescentis were not effective.…”

Section: Discussionmentioning

confidence: 99%

Culture Supernatants of Lactobacillus acidophilus and Bifidobacterium adolescentis Repress Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug by Suppressing Unbalanced Growth of Aerobic Bacteria and Lipid Peroxidation

Kinouchi

Kataoka

Bing

et al. 1998

Microbiology and Immunology

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A nonsteroidal antiinflammatory drug, 5-bromo-2-(4-fluoropheny1)-3-(4-methylsulfonylphenyl) thiophene (BFMeT), induced ileal ulcers in rats after oral administration, while no ulcers were observed after subcutaneous injection. The ileal ulcer formation in BFMeT-treated rats was examined to correlate the administration of cultures of Lactobacillus acidophilus or Bifidobacterium adolescentis with intestinal bacteria in the ileal contents and lipid peroxidation of the small intestinal mucosa. Ileal ulcers were observed in more than 85% of the rats treated with BFMeT at a dose of 1,000 mg/kg when they were given tap water as drinking water. The incidence of ulcer formation was repressed by giving culture supernatants of L. acidophilus or B. adolescentis as drinking water, but not by giving the cell suspension as drinking water. Gram staining of the ileal contents of normal rats revealed that 97 % of the stained bacteria were Gram-positive rods and only 1.5% were Gram-negative rods. The percentage of Gram-negative rods 72 hr after BFMeT administration was 49.8% and increased over 30-fold in BFMeT-treated rats. However, the percentage of Gram-negative rods was 9.7% or 16%, respectively, in rats taking culture supernatants of L. acidophilus or B. adolescentis. In addition, thiobarbituric acid-reactive substances in the ileal mucosa increased significantly in the rats given tap water for 72 hr after BFMeT treatment, but not in rats given the culture supernatants of L. acidophilus or B. adolescentis. Since BFMeT induced an unbalanced intestinal microflora, the effect of antibiotic treatment on ulcer formation in rats was examined. The magnitude of the ulcer formation in the antibiotic-treated rats was, in decreasing order, metronidazole > none > kanamycin > a mixture (bacitracin, neomycin and streptomycin). These results suggest that the intestinal microflora plays an important role in ulcer formation and that a metabolite(s) of L. acidophilus and B. ado-, lescentis inhibits ileal ulcer formation by repressing changes in the intestinal microflora and lipid peroxidation in BFMeT-treated rats.

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Section: Discussionmentioning

confidence: 99%

Culture Supernatants of Lactobacillus acidophilus and Bifidobacterium adolescentis Repress Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug by Suppressing Unbalanced Growth of Aerobic Bacteria and Lipid Peroxidation

Kinouchi

Kataoka

Bing

et al. 1998

Microbiology and Immunology

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“…9,10) EPSs have been shown to benefit human health by their immunomodulation, anti-gastritis, anti-ulcer, and anti-virus activities. [11][12][13] We have shown that P. pentosaceus LP28 has the strain-specific EPS biosynthetic gene cluster, 14) and intake of the strain is effective to improve the high fat diet-induced obesity and fatty liver of the mice. 6) Furthermore, the strain is also useful to reduce body fat and body weight in human clinical study.…”

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confidence: 99%

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

Panthavee

Noda

Danshiitsoodol

et al. 2017

Biological & Pharmaceutical Bulletin

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In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plantderived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC 50 values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources. Key words exopolysaccharide; Lactobacillus amylovorus; Pediococcus pentosaceus; thermophilic lactic acid bacteria; anti-inflammatory substanceTraditionally, lactic acid bacteria (LAB) have been useful for producing fermented foods, such as yogurt, kimchi, and cheese. LAB-Gram-positive bacteria-are non-pathogenic and are generally recognized as safe (GRAS) microorganisms; therefore, they have been the focus of attention for their industrial importance. 1)Microorganisms that contribute to human health are called probiotics. This word is internationally defined as "living microorganisms conferring a health benefit on the host when administered in adequate amounts."2) We have isolated many kinds of LAB strains from plant sources such as vegetables, fruits, flowers, and medicinal plants to establish a plantderived LAB library with more than 600 strains. We have demonstrated that some strains stocked in the LAB library are useful for enhancing intestinal immunity, improving liver function, and preventing metabolic syndrome. [3][4][5][6] LAB fermentation often proceeds even at low temperatures, causing the overgrowth of LABs. As the result, the degradation of flavors is proceeded and affected with taste of the fermented foods. Since thermophilic LAB strains hard to ferment at lower temperatures, therefore, if the thermophile ones are successfully isolated, they will contribute to prevent over-fermentation. In the present study, we isolated 28 strains of thermophilic LAB from tropical fruits of Thailand. In the isolates, we found two exopolysaccharide (EPS)-producing LAB strains. The LY45 strain was isolated from lychee, and the PY45 strain was obtained from pineapple. These strains were identified as Pediococcus (P.) pentosaceus and Lactob...

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“…Uejima et al (18) founded that Lactobacillus inhibited non-steroidal anti-inflammatory druginduced ileal ulcer in rats by repressing the growth of ulcer-inducing bacteria, the Gram-negative facultative anaerobic rods Esherichia coli, Klebsiella, and Proteus, although the ulcer models were different than the models used in the present study or Elliott's model. On the other hand, Nagaoka et al (19) reported that polysaccharide of Lactobacillus cell wall had a gastroprotective effect and the content of rhamnose was important. Indeed, in this study, non-fermented milk did not inhibit the lesion formation.…”

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confidence: 99%

Yogurt Containing Lactobacillus gasseri OLL2716 Exerts Gastroprotective Action Agaisnt Acute Gastric Lesion and Antral Ulcer in Rats

Uchida¹,

Kurakazu²

2004

J Pharmacol Sci

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Abstract. Yogurt containing Lactobacillus gasseri OLL2716 (LG21 yogurt) is reported to improve Helicobacter pylori-induced gastric mucosal inflammation in clinical studies. However, other beneficial effects of LG21 yogurt have not been clarified. Therefore, we examined whether LG21 yogurt exhibits a gastroprotective action against acute gastric lesion or antral ulcer in rats. Moreover, the mechanism of gastroprotective action was also evaluated. After fasting, acute gastric lesions were induced by 0.6 M HCl. Gastric mucosal folds were stained by oral administration of methyl violet. Antral ulcers were induced by the combined administration of diethyldithiocarbamate and HCl in refed rats after fasting. LG21 yogurt was orally administered before HCl treatment or staining the mucosal folds.LG21 yogurt significantly and dosedependently inhibited the formation of acute gastric lesions, and this gastroprotective action was attenuated by pretreatment with indomethacin. LG21 yogurt also significantly increased prostaglandin E 2 generation in the gastric mucosa. Stained length of gastric mucosal fold was reduced by LG21 yogurt. Antral ulcer formation was also significantly inhibited by LG21 yogurt. From the above results, it was found that the ingestion of LG21 yogurt is useful for the prevention of gastric ulcer. Moreover, endogenous prostaglandin was suggested to be one of the gastroprotective mechanisms of LG21 yogurt.

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Anti-ulcer Effects of Lactic Acid Bacteria and Their Cell Wall Polysaccharides.

Cited by 104 publications

References 2 publications

Culture Supernatants of Lactobacillus acidophilus and Bifidobacterium adolescentis Repress Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug by Suppressing Unbalanced Growth of Aerobic Bacteria and Lipid Peroxidation

Culture Supernatants of Lactobacillus acidophilus and Bifidobacterium adolescentis Repress Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug by Suppressing Unbalanced Growth of Aerobic Bacteria and Lipid Peroxidation

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

Yogurt Containing Lactobacillus gasseri OLL2716 Exerts Gastroprotective Action Agaisnt Acute Gastric Lesion and Antral Ulcer in Rats

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