Anti-trypanosomal and anthelminthic properties of ethanol and aqueous extracts of Tetrapleura tetraptera Taub

Obeng, Ahmed Wahab; Boakye, Yaw Duah; Agana, Theresa Appiah; Djameh, Georgina Isabella; Boamah, Daniel; Adu, Francis

doi:10.1016/j.vetpar.2021.109449

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…Again, only the ethanol extract could cause the death of worms ( Table 3 ). This might be due to the presence of phytochemicals such as tannins, saponins, phenolics, and terpenoids that have been reported by Tagoe et al [ 26 ] and Wahab Obeng et al [ 27 ] to possess anthelmintic activity. The bioactive components in the ethanol extract could be working in synergy or individually to cause paralysis or death in the worms [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Preliminary Screening for the Anthelmintic Activity of Millettia zechiana Harms and Its Modifying Effect on Albendazole

Asiamah,

Agana,

Boakye

et al. 2024

Journal of Parasitology Research

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Helminthic infections affect a greater proportion of the world’s population. This study determined the anthelmintic activity of Millettia zechiana and its modifying effect on albendazole. Powdered leaves of M. zechiana were successively extracted with petroleum ether, ethyl acetate, and ethanol. The anthelmintic potential of the M. zechiana leaf extracts and the modifying effects of the extracts on albendazole were determined on Pheretima posthuma. Phytochemical and gas chromatography-mass spectroscopy (GC-MS) analyses were performed to determine the chemical composition of each extract. The plant extracts of M. zechiana had few or all phytoconstituents such as tannins, saponins, flavonoids, glycosides, terpenoids, phytosterols, and alkaloids present. The IC50 obtained for albendazole, petroleum ether, ethyl acetate, and ethanol extracts for paralysis time were 0.936, 1.722, 1.283, and 1.348 mg/mL, respectively. The IC50 obtained for albendazole and the ethanol extract for death time were 4.638 and 4.988 mg/mL. The ethanol extract at 10 and 5 mg/mL caused death in the worms after 152.5±8.66 minutes and 304.8±7.27 minutes of exposure, respectively. Ethanol, ethyl acetate, and petroleum ether extracts of M. zechiana significantly modified the activity of albendazole at concentrations of 2.5 and 1.25 mg/mL (P<0.0001). The ethanol extract which exhibited the best anthelminthic activity was fractionated through column chromatography, and five (5) fractions were obtained. Fractions 1, 2, 4, and 5 had the best paralytic activities against the worms. Fractions 1 and 2 demonstrated better helminthicidal activity than albendazole, which had an IC50 of 3.915. The GC-MS analysis of the ethanol, ethyl acetate, and petroleum ether extracts showed the presence of 10, 10, and 37 compounds, respectively, with 9-octadecenamide, (Z)-, n-hexadecanoic acid, oleic acid, and some aromatic compounds being the most predominant. The results obtained indicate that M. zechiana leaf extract possesses anthelmintic activity.

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Section: Discussionmentioning

confidence: 99%

Preliminary Screening for the Anthelmintic Activity of Millettia zechiana Harms and Its Modifying Effect on Albendazole

Asiamah,

Agana,

Boakye

et al. 2024

Journal of Parasitology Research

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“…e observed activity could be attributed to the secondary metabolites found in the crude extracts. Flavonoids, triterpenoids, and tannins have been reported to exhibit antitrypanosomal activity [12,19]. Hence, the presence of tannins and triterpenoids in CPSe may account for its strong antitrypanosomal activity.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these plants are traditionally used to treat both helminth and trypanosome infection, hence serving as a better alternative for individuals infected with both parasites due to a higher chance of reduced undesirable side effects [17,18]. Ahmed et al [19] have reported that Tetrapleura tetraptera fruit has both antitrypanosomal and anthelminthic activity.…”

Section: Introductionmentioning

confidence: 99%

Antitrypanosomal and Anthelminthic Properties of Ethanol Extracts of Carica papaya Linn. and Ceiba pentandra (L) Gaertn.

Obeng

Boakye

Agana

et al. 2022

Journal of Chemistry

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The most common diseases that affect low-income countries are helminthosis and trypanosomosis. In Ghana, and in many other African countries, herbal treatment of various diseases is still common. In the present study, we sought to determine the antitrypanosomal and anthelminthic activities of Carica papaya and Ceiba pentandra. The ethanol extracts of Carica papaya stem bark (PPSe) and leaves (PPLe) and ethanol extracts of Ceiba pentandra stem bark (CPSe) and leaves (CPLe) were screened against Trypanosoma brucei brucei and Pheretima posthuma worms in vitro. CPSe exhibited strong antitrypanosomal activity, while the other extracts exhibited moderate activity against T. b. brucei. All the extracts showed weak selective indices (SI) when tested on Jurkat cell lines, which is indicative of a potential toxic effect. When the extracts were screened against P. posthuma worms, only PPSe and CPSe were able to kill the worms after the exposure time at concentrations of 2.5, 5, and 10 mg/mL. PPSe was again the only extract that potentiated the anthelminthic activity of albendazole against P. posthuma worms. Preliminary phytochemical screening and GC-MS analysis revealed the presence of compounds with antitrypanosomal and anthelminthic properties. The results confirmed the potential of C. papaya and C. pentandra as remedies for trypanosomosis and helminthosis and also gives credence to their folkloric use.

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“…An attenuated total reflectance infrared spectrophotometer was used to determine the number of functional groups present in the DO5 bioactive fraction (BF2124). Ten milligrams (10 mg) of dried fractions were mounted directly on the KBr disc of the PerkinElmer 200 UATR (FT-IR C9413) spectrophotometer [ 29 ] and scanned through the IR region between the ranges of 400 to 4000 cm −1 at a resolution of 4 cm −1 .…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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Microorganisms are one of the main sources of antimicrobial agents and over 50% of antibiotics currently used in hospitals are metabolites from microbes. This study aimed to isolate microorganisms from the Dompoase landfill site, Kwame Nkrumah University Physics Garden, Kosiko River, and Ada Foah seashore of Ghana and screen their metabolites for antimicrobial activity. Forty-eight (48) microorganisms were isolated and their metabolites were screened against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Salmonella typhi, Pseudomonas aeruginosa, Streptococcus pyogenes, Proteus mirabilis, and Candida albicans using the agar well diffusion method. Ten (10) of the isolates exhibited antimicrobial activity. Isolate DO5, identified as P. aeruginosa isolate, from the Dompoase landfill site was selected for fermentation because it exhibited the highest activity against all the test organisms. DO5 produced optimum antimicrobial activity when fermented for 11 days at 30°C. In the agar diffusion method, the extract of isolate DO5 recorded zones of inhibition ranging between 11.67 ± 0.23 and 21.50 ± 0.71 mm. The MIC and MBC recorded for the DO5 extract ranged from 3.13–25.0 mg/mL and from 6.25–50.0 mg/mL, respectively. Column chromatography analysis yielded eight (8) subfractions from the DO5 extract. IR analysis revealed the presence of functional groups such as alcohols, esters, and hydrocarbons in the fractions. GC-MS analysis identified nine compounds that have been reported to have antimicrobial agents. The DO5 metabolites stand the chance to be developed into potent antibiotics for infection treatment.

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Anti-trypanosomal and anthelminthic properties of ethanol and aqueous extracts of Tetrapleura tetraptera Taub

Cited by 13 publications

References 27 publications

Preliminary Screening for the Anthelmintic Activity of Millettia zechiana Harms and Its Modifying Effect on Albendazole

Preliminary Screening for the Anthelmintic Activity of Millettia zechiana Harms and Its Modifying Effect on Albendazole

Antitrypanosomal and Anthelminthic Properties of Ethanol Extracts of Carica papaya Linn. and Ceiba pentandra (L) Gaertn.

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

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