Anti-Trypanosomal Activity and Cytotoxicity of Some Compounds and Extracts from Nigerian Medicinal Plants

Gray, Alexander I.; Igoli, John O.; Clements, Carol; Mouad, Hazar

doi:10.5772/26203

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Only one of the nine investigated plants lacked inhibitory activity, P. schweinfurthii, whereas all other plants demonstrated at least one extract with a moderate antitrypanosomal activity. Of the nine plants, seven had already been investigated for their antitrypanosomal activity, namely B. owariensis, C. febrifuga, E. abyssinica, M. charantia, N. lotus, S. latifolius, and V. madiensis [10,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39]. To the best of our knowledge, B. schreberi (Table S1, extracts ID 96, 98, 109, 110, 111) is reported for its antitrypanosomal activity for the first time here.…”

Section: Screening Of Extracts Against Trypanosoma Brucei Rhodesiensementioning

confidence: 99%

Antitrypanosomal Constituents from Brasenia schreberi and Nymphaea lotus Used in a Single Herbal Preparation against Sleeping Sickness in Angola

Vahekeni,

Brillatz,

Rahmaty

et al. 2024

Preprint

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Folk medicine is widely used in Angola, even for human African trypanosomiasis (sleeping sickness) in spite of the fact that the reference treatment is available for free. Aiming to validate herbal remedies in use, we have selected nine medicinal plants and assessed their antitrypanosomal ac-tivity. 122 extracts were prepared using different plant parts and solvents. 15 extracts from seven different plants exhibited in vitro activity against Trypanosoma brucei rhodesiense bloodstream forms >70% at 20 µg/ml. The dichloromethane extract of Nymphaea lotus (leaves and leaflets) and the ethanolic extract of Brasenia schreberi (leaves) had IC50 values ≤10 µg/ml. These two aquatic plants are of particular interest. They are being co-applied in the form of a decoction of leaves because they are considered by local healers as male and female of the same species, the ethnotaxon “longa dia simbi”. Bioassay-guided fractionation led to the identification of eight active molecules: gallic acid (IC50 0.5 µg/ml), methyl gallate (IC50 1.1 µg/ml), 2,3,4,6-tetragalloyl-glucopyranoside, ethyl gallate (IC50 0.5µg/ml), 1,2,3,4,6-pentagalloyl-β-glucopyranoside (IC50 20µg/ml), gossypet-in-7-O-β-glucopyranoside (IC50 5.5µg/ml), and hypolaetin-7-O-glucoside (IC50 5.7µg/ml) in B. schreberi, and 5-[(8Z,11Z,14Z)-heptadeca-8,11,14-trienyl] resorcinol (IC50 5.3µg/ml) not described to date in N. lotus. Five of these active constituents were detected in the traditional preparation. This work provides the first evidence for the ethnomedicinal use of these plants in the management of sleeping sickness in Angola.

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Section: Screening Of Extracts Against Trypanosoma Brucei Rhodesiensementioning

confidence: 99%

Antitrypanosomal Constituents from Brasenia schreberi and Nymphaea lotus Used in a Single Herbal Preparation against Sleeping Sickness in Angola

Vahekeni,

Brillatz,

Rahmaty

et al. 2024

Preprint

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“…This was carried out as previously described (Igoli et al, 2011). The hexane, ethyl acetate and methanol extracts were screened for activity against blood stream form of Trypanosoma brucei brucei (T.b.brucei) S427 using an Alamar Blue TM assay.…”

Section: Antitrypanosomal Assay Drug Sensitivity Using Blood Stream F...mentioning

confidence: 99%

A New Isoflavone from Lomariopsis guineensis (Underw.) Alston

et al. 2023

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Aim/background: Lomariopsis guineensis (Underw.) Alston is an epiphytic climbing fern. It is widely distributed in Africa where it is also used in traditional medicine and as food. There are no previous reports of any constituents of the plant, hence this study to isolate any phytoconstituents. Method: The ethyl acetate extract of the leaves was subjected to column chromatography and isolated constituents were characterized using nuclear magnetic resonance and mass spectrometry. Results: Three compounds were isolated and identified as cycloartenol, pheophytin A and a new isoflavone (5, 7-dihydroxy-4′ methoxy-6,8-dimethylisoflavone). Conclusion: Three phytochemicals including a new isoflavone are reported from the plant for the first time. Keywords: Isoflavone; Lomariopsis guineensis; pheophytin A; cycloartenol; antitrypanosomal assay

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“…Also, some parasitic diseases require high doses for long terms of drugs with substantial adverse effects. Therefore, it is not surprising that researchers are interested in healing parasitic infections with plant remedies with proven effectiveness [39]. Traditional herbal healing of parasitosis rely on well know plants like garlic, ramson or pumpkin seeds, but interesting studies show that innovative technologies can bring new insights into treating parasitic diseases [40,41].…”

Section: Phytotherapy In Parasitosismentioning

confidence: 99%

Are Herbal Products an Alternative to Antibiotics?

Ionescu¹

2018

Bacterial Pathogenesis and Antibacterial Control

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Medicinal plants have been widely used in the management of infectious diseases and by now, many of the ancient remedies have proven their value through scientific methodologies. Although the mechanisms underlying most plant-derived remedies are not well understood, the success of herbal medicine in curing infectious diseases shows that many plants have beneficial effects in various bacterial, fungal, viral or parasitic infections. The modern methodologies in the isolation, purification and characterization of the active compounds, has been a great impact for advancing in vitro and in vivo research, this step being crucial for further application in clinical trials. Many plant-derived compounds, for example, quinine and artemisinin, have been already successfully used in healing lifethreatening infectious disease. The main limitations of plant medicine healing are lack of standardization and reproducibility of plant-derived products. Despite the paucity of clinical trials evaluating their efficacy, phytotherapy, adult plant uses and gemmotherapy, the use of embryonic stem cells should be reconsidered as valuable resources in finding new active compounds with sustained antimicrobial activity.

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Anti-Trypanosomal Activity and Cytotoxicity of Some Compounds and Extracts from Nigerian Medicinal Plants

Cited by 4 publications

References 25 publications

Antitrypanosomal Constituents from <em>Brasenia schreberi</em> and <em>Nymphaea lotus</em> Used in a Single Herbal Preparation against Sleeping Sickness in Angola

Antitrypanosomal Constituents from <em>Brasenia schreberi</em> and <em>Nymphaea lotus</em> Used in a Single Herbal Preparation against Sleeping Sickness in Angola

A New Isoflavone from Lomariopsis guineensis (Underw.) Alston

Are Herbal Products an Alternative to Antibiotics?

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