Anti-Trypanosoma cruzi antibody detection in eastern Andalusia (Spain)

Abstract

The excellent specificity of SODe antigen for the detection of antibodies to T. cruzi in donors lead us to affirm that the serological test performed with this biomarker could provide a useful screening and confirmatory test method for cases of Chagas disease.

Usefulness of the ARCHITECT Chagas® assay as a single test for the diagnosis of chronic Chagas disease

“…Some 87 000 of these immigrants are estimated to be potentially infected with T. cruzi , which means that screening this population could provide a clue to treating and preventing cardiac and digestive complications and avoiding congenital transmission . Spanish studies have recorded the seroprevalence of Chagas disease among Latin American immigrants as ranging from 2.5% to 22.3% , while our study obtained a seroprevalence of 7.39%. Compared with other European countries, our seroprevalence was not as high as previously reported .…”

Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis

“…Thirty-four studies were selected for the ELISA technique: [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ] in which a total of 6054 subjects were studied. Sensitivity ranged from 78.0% to 100%, with a median of 99.0%, CI 95% (94, 100), while the test for equality of sensitivities presented a χ 2 = 657.24, df = 33, p -value = 2 × 10 −16 .…”

“…Sensitivity and specificity are reported with a mean (95% confidence limits). The Forest plot depicts the estimated sensitivity and specificity (black squares) and its 95% confidence limits (horizontal black line) [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ].…”

Analysis of iron superoxide dismutase‐encoding DNA vaccine on the evolution of the Leishmania amazonensis experimental infection

“…BALB/c mice were also protected from the challenge with Brucella abortus following intrasplenic immunization with the pcDNA‐SOD vaccine , as this type of immunization triggered an immunological response associated with the Th1 immune response, and cytotoxic T lymphocytes able to eliminate intracellular bacteria . Trypanosoma cruzi iron SOD also appears to be immunogenic, as dogs that were naturally infected produced anti‐SOD antibodies . This suggests that independent of the microbe source, the SOD antigen is naturally immunogenic given that SOD proteins from different parasites have structural identity, suggesting similar mechanism of immunity.…”