Anti-triiodothyronine auto-antibody interference in recent free thyroid hormone assays

Sapin, R; Schlienger, Jean-Louis; Gasser, F; Chambron, J.

doi:10.1016/0009-9120(95)02012-8

Cited by 16 publications

(4 citation statements)

References 9 publications

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“…Os THAAb se ligam ao T 4 e T 3 marcado e ao T 4 e T 3 da amostra levando a resultados falsamente elevados ou diminuídos de T 4 e T 3 livre e/ou total dependendo do método utilizado (118,122). O tipo de interferência do THAAb não só depende do método utilizado, mas também da sua afinidade pelo HT e concentração na amostra (123)(124)(125). No caso de suspeita de interferência com THAAb, deve-se fazer uma avaliação do TSH, dosar HT com um método comparativo e se necessário utilizar uma das técnicas para remover ou identificar o THAAb (precipitação com polietileno-glicol ou radioimunoensaio) (118).…”

Section: Fatores Interferentes Nos Ensaios De T 4 E T 3 Total E Livreunclassified

Fatores Interferentes na Interpretação de Dosagens Laboratoriais no Diagnóstico de Hiper e Hipotireoidismo

Graf

Carvalho

2002

Arq Bras Endocrinol Metab

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Laboratory Interfering Factors in the Diagnosis of Hyper and Hypothyroidism.Since the first reports in the medical literature describing the clinical presentation of hypo and hyperthyroidism, very little has changed on the semiologic scenario of these entities and even in their therapeutic approach. The changing trends refer much more to the tools used to diagnose thyroid disease. In parallel with these developments, we understand much better about the factors that interfere in the interpretation of thyroid function tests in the diagnosis of hyper and hypothyroidism. In this article, we analyzed the utility of serum TSH and thyroid hormone measurements, as well the pitfalls and interferences in its common daily use. (Arq Bras Endocrinol Metab 2002;46/1:51-64)

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Section: Fatores Interferentes Nos Ensaios De T 4 E T 3 Total E Livreunclassified

Fatores Interferentes na Interpretação de Dosagens Laboratoriais no Diagnóstico de Hiper e Hipotireoidismo

Graf

Carvalho

2002

Arq Bras Endocrinol Metab

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“…All experiments suggest the presence of a high-molecular weight interference, which we hypothesize to be endogenous antibodies that may be specific to T 3 (3). Regardless of the mechanism, the results reveal that the Vitros reagent is insufficiently formulated to accurately measure endogenous T 3 .…”

Section: To the Editormentioning

confidence: 86%

False-Positive Total T3 Using the Ortho Vitros Immunoassay in Pediatric Populations

Dickerson

Polsky

Greene

et al. 2017

The Journal of Applied Laboratory Medicine

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“…We calculated a correction factor for this effect and after applying this factor, we were able to get the results very close to that obtained by using Immunotech kit, in which the effect of antithyroid hormone antibodies is known to be minimal (Table 3). Recently, Sapin et.aL (13) have suggested that the percent interference calculated as ratio of the radioactivity bound by patient's serum in the presence and absence of the interfering immunogiobulins correlates better with the assay results than the percent binding of tracer as such. In the present case, the interference in the assay was found to be 85.3%.…”

Section: Resultsmentioning

confidence: 99%

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

Rothman

Chawla

1998

Indian J Clin Biochem

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The thyroid hormone profile of a hypothyroid patient, with moderate sized diffuse goiter and history of exogenous thyroid hormone therapy, was found to be highly ambiguous. The total as well as free T4 levels were consistently high (out of range) with suppressed T3 (total and free) and elevated thyrotropin levels. We incubated the serum with the radio-iodine labelled T4 and observed that the patient's serum bound about 50.5% of the total radioactivity resulting in 85.3% interference in the radioimmunoassay. On resolving the serum proteins by cellulose acetate electrophoresis, the radioactivity was detected in the gamma globulin bend. The protein profile showed mildly raised levels of IgG. Therefore it was established that the patient's serum carried T4 reactive antibodies (IgG) which were interfering in the immunoassays. Elevated anti-thyroid microsomal antibodies were also present in the patient's serum. The anti-T4 antibodies were highly specific for T4 and did not show any cross reactivity with the T3 or its analogues used in the estimation of free T3. We stripped the serum by precipitating the immunoglobulins and were successful in estimating the levels of free T4 in the stripped serum by single step radioimmunoassy.

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Anti-triiodothyronine auto-antibody interference in recent free thyroid hormone assays

Cited by 16 publications

References 9 publications

Fatores Interferentes na Interpretação de Dosagens Laboratoriais no Diagnóstico de Hiper e Hipotireoidismo

Fatores Interferentes na Interpretação de Dosagens Laboratoriais no Diagnóstico de Hiper e Hipotireoidismo

False-Positive Total T3 Using the Ortho Vitros Immunoassay in Pediatric Populations

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

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