Anti-Toxoplasma gondii antibodies attach to mouse cancer cell lines but not normal mouse lymphocytes

Abstract

Toxoplasma gondii (T. gondii) is prevalent intracellular parasite and a cause of worldwide infection in the human population. An inhibitory effect of this parasite on cancer growth has been demonstrated in cell culture and animal models. To determine whether the anticancer activities of T. gondii are associated with host immune response, in the current study the reactivity of anti-T. gondii antiserum with the surface of cancer cell lines was investigated. Anti-T. gondii antibodies were raised in rabbit and the reaction of this antiserum in comparison with other anti-parasite antisera (anti-T. vaginalis, anti-hydatid cyst fluid, anti-protoscolices antigens) with mouse melanoma or breast cancer cells lines was investigated using flow cytometry. Anti-T. gondii antiserum reacted markedly with the surface of mouse melanoma and breast cancer cells, and less so with the normal mouse spleen lymphocytes. Meanwhile, the other anti-parasite antisera did not react strongly with the surface of cancer cells compared with normal mouse spleen lymphocytes. In summary, it has been demonstrated herein that anti-T. gondii antiserum may selectively react with the surface of mouse cancer cells but not with normal mouse spleen lymphocytes. Therefore, further study on anti-Toxoplasma antibodies may be useful for directing the application of selective drug delivery in cancer treatment.

Effect of Toxoplasma gondii on colon cancer growth in mouse model

“…This study showed that treatment of Tumor bearing mice with Toxoplasma gondii lysate or irradiated tachyzoite resulted in decreasing tumor volume. In agreement with results of this investigation, there are scientific evidences demonstrating that T.gondii have desired effect on tumor suppression [5][6][7][8][9]. In experiments with Toxoplasma gondii it has been shown that injection of live tachyzoites of this parasite resulted in decreasing growth of lung [15] and melanoma [16] cancers in mice.…”

“…Moreover, there are a lot of evidences about anti-cancer effects of parasites in human population, in animal model and in laboratory experiments [3,8,10,11].…”

“…There are many evidences demonstrating that Toxoplasma gondii can stimulate immune system and has positive effects in cancer treatment [6][7][8]. Recent studies have shown that T.gondii when loss its replication capacity can't cause symptoms despite it invades cells.…”

“…Recent studies have shown that T.gondii when loss its replication capacity can't cause symptoms despite it invades cells. In these situation immune system raised against the parasite may have anti-tumor effects [6][7][8]. So, in this work effect of Toxoplasma gondii tachyzoite on growth of mouse colon cancer has been investigated.…”

The Recombinant Protein Based on Trypanosoma cruzi P21 Interacts With CXCR4 Receptor and Abrogates the Invasive Phenotype of Human Breast Cancer Cells

“…Several studies involving parasites demonstrate that bioactive molecules and parasites promote antitumor effects, such as Strongyloides stercoralis, Toxoplasma gondii, Plasmodium, and Trypanosoma cruzi (Plumelle et al, 1997;Kim et al, 2007;Chen et al, 2011;Lukasiewicz and Fol, 2018). It has been demonstrated that parasite anticancer activity is mediated by antitumor immunity induction and immunomodulation (Ubillos et al, 2016;Mohamadi et al, 2019;Riaz, 2019), gene regulation (Lu et al, 2019), and anticancer effects by parasite molecule production (Atayde et al, 2008;Valck et al, 2010;Darani and Yousefi, 2012;Ramírez et al, 2012).…”