Anti-Sporozoite Antibodies Induced by Natural Infection *

Tapchaisri, Pramuan; Chomcharn, Y; C, Poonthong; Asavanich, Achara; Limsuwan, S; Maleevan, Orvadee; Tharavanij, S; Harinasuta, T

doi:10.4269/ajtmh.1983.32.1203

Cited by 31 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also examined for statistical interactions between anti-CSP antibody titres and cellular responses in determining risk of clinical malaria (Table 2). However, we could not examine these interactions among the control vaccinees, since antibody responses were detectable in less than 1% of this group, confirming earlier reports that natural exposure to malaria does not induce durable levels anti-CSP antibodies [20], [21]. The interaction between TNF+ CD4+ T cells and anti-CSP antibody titers is shown graphically in figure 2, which is based on medians, interquartile ranges, and 5 th to 95 th centiles.…”

Section: Resultssupporting

confidence: 68%

A Statistical Interaction between Circumsporozoite Protein-Specific T Cell and Antibody Responses and Risk of Clinical Malaria Episodes following Vaccination with RTS,S/AS01E

et al. 2012

View full text Add to dashboard Cite

The candidate malaria vaccine RTS,S/AS01E provides significant but partial protection from clinical malaria. On in vitro circumsporozoite protein (CSP) peptide stimulation and intra-cellular cytokine staining of whole blood taken from 407 5–17 month-old children in a phase IIb trial of RTS,S/AS01E, we identified significantly increased frequencies of two CSP-specific CD4+ T cells phenotypes among RTS,S/AS01E vaccinees (IFNγ-IL2+TNF− and IFNγ-IL2+TNF+ CD4+ T cells), and increased frequency of IFNγ-IL2-TNF+ CD4+ T cells after natural exposure. All these T cells phenotypes were individually associated with reductions in the risk of clinical malaria, but IFNγ-IL2-TNF+ CD4+ T cells independently predicted reduced risk of clinical malaria on multi-variable analysis (HR = 0.29, 95% confidence intervals 0.15–0.54, p<0.0005). Furthermore, there was a strongly significant synergistic interaction between CSP-specific IFNγ-IL2-TNF+ CD4+ T cells and anti-CSP antibodies in determining protection against clinical malaria (p = 0.002). Vaccination strategies that combine potent cellular and antibody responses may enhance protection against malaria.

show abstract

Section: Resultssupporting

confidence: 68%

A Statistical Interaction between Circumsporozoite Protein-Specific T Cell and Antibody Responses and Risk of Clinical Malaria Episodes following Vaccination with RTS,S/AS01E

et al. 2012

View full text Add to dashboard Cite

show abstract

“…[5][6][7] One report has illustrated the application of the indirect fluorescence antibody test (IFAT) for retrospective diagnosis of malaria in travelers who took stand-by antimalarial treatment. 8 Antibody responses to sporozoites have been studied extensively in immune populations [9][10][11] and in nonimmune individuals, 12,13 mostly by ELISAs based on synthetic or recombinant peptides from the repetitive domain of the circumsporozoite (CS) protein. Recently, a retrospective study reported a high prevalence (21%) of anti-CS antibodies in asymptomatic travelers returning from sub-Saharan Africa.…”

mentioning

confidence: 99%

Testing for anti-circumsporozoite and anti-blood-stage antibodies for epidemiologic assessment of Plasmodium falciparum infection in travelers.

Cobelens

Verhave²,

Leentvaar-Kuijpers³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The purpose of this investigation was to assess the role of serology for establishing incidences of Plasmodium falciparum malaria and of exposure to P. falciparum in epidemiologic studies of travelers using chemoprophylaxis. The design was a prospective cohort study involving 548 short-term Dutch travelers to areas endemic for P. falciparum malaria. Sera were collected before departure and, together with the medical history, 2-6 weeks after return. All sera were tested for anti-circumsporozoite (CS) antibodies by an R32tet 32 -ELISA; sera of subjects reporting febrile illness during travel or after return or with anti-CS responses were tested for anti-blood-stage antibodies by an indirect fluorescence antibody test (IFAT). Five subjects (0.9%) reported P. falciparum malaria confirmed by thick blood smear examination (documented cases) and six (1.0%) reported treatment for malaria without a documented diagnosis (presumptive cases). Conversions in the IFAT were detected in six subjects, including all five documented cases and one presumptive case. Anti-CS antibodies were detected in seven subjects (1.3%), including three documented cases and four of 442 subjects with no history of fever or malaria treatment (0.9%). Incidence rates per 1,000 person-months of travel (95% confidence interval) of infection with P. falciparum, whether or not suppressed by chemoprophylaxis, were 16.9 (8-31) for all destinations and 91.6 (33-200) for West Africa. In epidemiologic studies of P. falciparum malaria in travelers, testing for antibodies to blood stages can increase the sensitivity and specificity of case detection; testing for antibodies to sporozoites may be useful for the assessment of exposure to P. falciparum in travelers using chemoprophylaxis, but the sensitivity is limited.In most epidemiologic studies that assess risks of Plasmodium falciparum malaria or efficacies of prophylactic measures in nonimmune travelers, incidences are based retrospectively on cases of malaria that are confirmed by blood slide evaluation.

show abstract

“…However, the prevalence of antibodies to P. falciparum sporozoites in Zacarõ Â as (18% ) was lower than that in regions of high-level transmission in Africa, Asia and Brazil (Nardin et al, 1979;Tapchaisri et al, 1983;Druilhe et al, 1986;Esposito et al, 1988;De Arruda et al, 1989). In an holo-endemic area of Burkina Faso (Druilhe et al, 1986), not only was the prevalence of anti-sporozoite antibodies close to 100% but the titres (1; 3200± 1; 6400) were also much higher than those seen in Zacarõ Â as (1; 20± 1; 320).…”

Section: Discussionmentioning

confidence: 93%

Unstable, low-level transmission of malaria on the Colombian Pacific Coast

GONZALEZ¹

1997

Annals of Tropical Medicine And Parasitology

View full text Add to dashboard Cite

The development of immune responses to malarial infection in inhabitants of endemic areas differs according to the level of exposure to the parasite. Adults living in a region where the level of malaria transmission is low (Colombia) have been shown to exhibit a similar response to each of the three regions of the circumsporozoite protein (the central repeated NANP region, and the flanking N- and C-termini). Conversely, donors exposed to a frequent sporozoite challenge in areas of high malaria transmission (Mali) exhibit antibodies predominantly to the NANP repeated domain. Malaria in the people of Zacarías, a community on the Pacific Coast of Colombia where malaria transmission is low and unstable, was the subject of the present study. Within a 9-year period, a negative correlation between rainfall and documented malaria cases was recorded for this area. Thick smears of blood samples of 319 individuals revealed that 8.5% had malarial infections. As most (67%) of the smear-positive cases were asymptomatic, it seems that, despite the low prevalence of malaria in this area, the establishment of clinical symptoms is attenuated, probably because of the acquisition of premunition. Within this region, the most commonly found Anopheles species (representing 61.1% of the mosquitoes caught) and that giving the highest monthly biting rate (4.0 bites/man) was An. neivai. Most (90%) of the human sera tested possessed antibodies to blood-stage forms of Plasmodium falciparum, and 18% had antibodies to sporozoites. More than half (58%) of the adults had been in contact with hepatitis B virus, 7.2% carried hepatitis B surface antigen, and syphilis was common but no subject was found to be seropositive for HIV. A better understanding of the dynamics of the different elements influencing malaria in areas of low, unstable transmission, such as the one described here, is essential for the design of new malaria-control strategies.

show abstract

Anti-Sporozoite Antibodies Induced by Natural Infection *

Cited by 31 publications

References 0 publications

A Statistical Interaction between Circumsporozoite Protein-Specific T Cell and Antibody Responses and Risk of Clinical Malaria Episodes following Vaccination with RTS,S/AS01E

A Statistical Interaction between Circumsporozoite Protein-Specific T Cell and Antibody Responses and Risk of Clinical Malaria Episodes following Vaccination with RTS,S/AS01E

Testing for anti-circumsporozoite and anti-blood-stage antibodies for epidemiologic assessment of Plasmodium falciparum infection in travelers.

Unstable, low-level transmission of malaria on the Colombian Pacific Coast

Contact Info

Product

Resources

About