Anti-Skin Inflammatory and Anti-Oxidative Effects of the Neoflavonoid Latifolin Isolated from Dalbergia odorifera in HaCaT and BJ-5ta Cells

Abstract

Skin is the first line of defense in the body against external stimulation and injury. Inflammation and oxidative stress in skin cells are the initiators and promoters of several skin diseases. Latifolin is a natural flavonoid isolated from Dalbergia odorifera T. Chen. This study aimed to evaluate the anti-inflammatory and antioxidant properties of latifolin. The anti-inflammatory effects were evaluated using tumor necrosis factor-α/interferon-γ (TNF-α/IFN-γ)-treated HaCaT cells, revealing that latifolin inhibited the secretion of Interleukin 6 (IL-6); Interleukin 8 (IL-8); Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES); and Macrophage-derived chemokine (MDC) while decreasing the expression of Intercellular Adhesion Molecule 1 (ICAM-1). The results of western blots and immunofluorescence demonstrated that the activation of Janus kinase 2 (JAK2), Signal transducer and activator of transcription 1 (STAT1), Signal transducer and activator of transcription 3 (STAT3), and nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) cells signaling pathways were significantly inhibited by latifolin. The antioxidant properties were evaluated using t-BHP-induced BJ-5ta cells. Latifolin increased the viability of t-BHP-induced BJ-5ta cells. Additionally, fluorescent staining of reactive oxygen species (ROS) showed that the production of ROS was inhibited by latifolin. Additionally, latifolin reduced the phosphorylation of p38 and JNK. The results indicate that latifolin has potential anti-inflammatory and antioxidant properties, and may be a candidate natural compound for the treatment of skin diseases.

Optimization of Antioxidant Activity of Compounds Generated during Ginseng Extract Fermentation Supplemented with Lactobacillus

“…To further evaluate the in vitro biological activity of the GS and GF, we used two human cell lines, HaCaT (keratinocytes) and BJ (fibroblasts), which are commonly used to evaluate the effects of antioxidant compounds on skin [ 55 , 56 ]. Specifically, we conducted cell viability assays to determine the effect of the GS and GF on the growth of HaCaT and BJ cells.…”

Kahweol Inhibits Pro-Inflammatory Cytokines and Chemokines in Tumor Necrosis Factor-α/Interferon-γ-Stimulated Human Keratinocyte HaCaT Cells

“…Once activated, keratinocytes produce numerous cytokines, including IL-1β and IL-6, which intensify the inflammatory response through autocrine and paracrine actions [10,39]. Moreover, when HaCaT cells are co-stimulated with TNF-α and IFN-γ, it triggers the generation of Th-2-related chemokines such as CXCL8 and MDC [40,41]. These chemokines are crucial The HaCaT cells underwent a pre-treatment with 10 µM kahweol for 1 h, followed by stimulation using TNF-α/IFN-γ (10 ng/mL each) for 2 h. Nuclei were visualized using DAPI.…”

“…Once activated, keratinocytes produce numerous cytokines, including IL-1β and IL-6, which intensify the inflammatory response through autocrine and paracrine actions [10,39]. Moreover, when HaCaT cells are co-stimulated with TNF-α and IFN-γ, it triggers the generation of Th-2-related chemokines such as CXCL8 and MDC [40,41]. These chemokines are crucial in recruiting inflammatory cells to skin lesions, thus contributing to the progression from acute to chronic inflammatory responses [42].…”