Anti-senescence efficacy of radio-electric asymmetric conveyer technology

Abstract

Recent evidence suggests that ageing-related diseases could result in an accelerated loss of self-renewal capability of adult stem cells, normally involved in replacing damaged cellular elements. In previous works, we highlighted that a specific treatment, named tissue optimization-regenerative (TO-RGN), of radio-electric asymmetric conveyer (REAC) technology, influenced gene expression profiles controlling stem cell differentiation and pluripotency of human skin-derived fibroblasts in vitro. The purpose of the present work was to verify whether TO-RGN may also be effective in counteracting the expression of the senescence marker beta-galactosidase and of senescence-associated gene expression patterning, engaged during prolonged culture of human adipose-derived stem cells (hADSCs). Following TO-RGN exposure, we observed a significant downregulation in beta-galactosidase staining and in the expression of the senescence mediator genes p16INK4, ARF, p53, and p21CIP1. Moreover, differently formed untreated cells, TO-RGN-exposed hADSCs maintained their typical fibroblast-like morphology and exhibited a multilineage potential even at late passages, as shown by the remarkable preservation of commitment to osteogenic, adipogenic, chondrogenic, and vasculogenic fates, both at morphologic and gene expression levels. In conclusion, our study highlights a positive effect of TO-RGN in counteracting degenerative senescence processes in vitro.

Stem cell senescence. Effects of REAC technology on telomerase-independent and telomerase-dependent pathways

“…Furthermore, REAC TO-RGN exposure proved effective in antagonizing the aging process in vitro 333435. Here, we show that REAC TO-RGN exposure also affected telomerase-dependent senescence mechanisms, by transcriptionally enhancing the expression of TERT, and counteracting telomere shortening.…”

Unravelling Cellular Mechanisms of Stem Cell Senescence: An Aid from Natural Bioactive Molecules

“…For these reasons, researchers in recent years have been trying to dissect the epigenetic modifications involved in cellular aging, in an attempt to reverse stem cell senescence and achieve rejuvenation [32,33]. Physical stimuli such as electromagnetic fields have been largely applied to slow down cell senescence, by modulating cell polarity and the expression of the aging related markers, such as TERT, p16INK4, ARF, p53, and p21, together with the activity of beta-galactosidase [21,[34][35][36]. Moreover, other authors demonstrated that Rapamycin can inhibit mTOR and its signalling pathway, restoring both the self-renewal and the potency of stem cells [37].…”

Cancer Stem Cells: Foe or Reprogrammable Cells for Efficient Cancer Therapy?

“…This effect resulted from and was associated with (i) the activation of a telomerase dependent pathway, linked to the re-expression of TERT, the gene coding for the catalytic core of telomerase with subsequent increase in telomere length [77], (ii) the induction of a telomerase independent pathway associated with the activation of Bmi-1 and the transient increase in the expression of pluripotency genes, such as Nanog, Sox2 and Oct4 [77], and (iii) the resumption of multilineage differentiation potential, as shown by recovery of high throughput of differentiation along vasculogenic, osteogenic, and adipogenic fates [78].…”