Anti-Quorum Sensing and Antioxidant Activity of Essential Oils Extracted From Juniperus Species, Growing Spontaneously in Tebessa Region (East of Algeria)

Abstract

The chemical composition of essential oils (EOs) extracted from the aerial parts of 2 species of Juniperus was determined by Gas Chromatography-Mass Spectrometry (GC-MS). In total, 65 and 58 compounds accounting for 90.3% and 89.8% of the whole chemical composition of Juniperus oxycedrus (JO) and Juniperus phoenicea (JP) were identified, respectively, with α-pinene, α-amorphene, terpinen-4-ol, α-terpinene, and β-elemene, as major components. For the first time, the capacity to inhibit quorum-sensing for Chromobacterium violaceum CV026 and CV12472 by the investigated EOs was evaluated. Both oils exhibited good violacein inhibition on CV12472 with 100.0 ± 0.0% inhibition at minimal inhibition concentration (MIC) values. Besides, the quorum-sensing inhibition of CV026 was high at MIC for JO essential oil from fruits (JOF, 16.3 ± 2.0 mm), JO leaves (JOL, 12.5 ± 3.5 mm), JP fruits (JPF, 19.7 ± 2.5 mm), and JP leaves (JPL, 21.1 ± 5.0 mm). On both CV12472 and CV026, essential oil from J. phoenicea leaves was the most active inhibitor. All investigated EOs inhibited swarming motilities in flagellated Pseudomonas aeruginosa (PA01) in a concentration-dependent manner, and those from JP were more active than EOs from JO. Moreover, these EOs showed good antioxidant potential according to DPPH● and FRAP methods, especially the EO from JO leaves with an IC50 DPPH● inhibition value of 20.2 ± 1.0 mg/mL. Based on the obtained results, the investigated EOs are good candidates to combat microbial resistance be used as alternatives to conventional antibiotics, and equally find applications in food biosafety as preservatives.

Antibiofilm and Anti-Quorum Sensing Potential of Cycloartane-Type Triterpene Acids from Cameroonian Grassland Propolis: Phenolic Profile and Antioxidant Activity of Crude Extract

“…Quorum sensing involves a cell–cell communication process in bacteria through the secretion, detection, and response to extracellular signaling molecules, which enable the bacterial community to monitor information about the environment, population, and cell numbers and collectively alter their gene expression, helping them to act in synchrony [ 63 ]. This accounts for the virulence factors, biofilms, and severity during infections; therefore, it will be beneficial to inhibit and disrupt quorum sensing networks in pathogenic bacteria, especially with the use of natural medicinal plants and their compounds and essential oils [ 9 , 64 , 65 ]. The propolis sample and the isolated pentacyclic triterpenoids inhibited violacein production and quorum sensing in C. violaceum CV12472 and C. violaceum CV026, respectively, indicating that they can reduce bacterial cell-to-cell communication networks.…”

Effects of Rubus fruticosus and Juniperus oxycedrus derivatives on culturability and viability of Listeria monocytogenes

“…Juniperus oxycedrus has, for example, traditional history of use in therapeutic and folk medicine for different diseases, such as tuberculosis, pneumonia, bronchitis, diarrhea, stomach aches, and hyperglycemia, but the data available on the chemical composition and biological activity are scarce 16 , 17 . Extracts of juniper species have been widely studied in relation to antioxidant activity 18 as well as antimicrobial potential 19 – 21 . Regarding its chemical profiles and biological activity, most research has been focused on EOs, in which α-pinene is usually the major constituent, followed by myrcene, sabinene, limonene, germacrene D, δ-cadinene and other terpenes and terpenoids.…”

Essential Oils and Extracts of Juniperus macrocarpa Sm. and Juniperus oxycedrus L.: Comparative Phytochemical Composition and Anti-Proliferative and Antioxidant Activities

“…In particular, the highest DPPH radical scavenging potential was observed with J. oxycedrus fruit essential oil, with an IC 50 value of 20.2 mg/mL, followed by J. phoenicea leaves (36.1 mg/mL). At a concentration of 9.2 mg/mL, all tested essential oils were able to reduce Fe 3+ to Fe 2+ [36].…”