Anti-quorum sensing and antibiofilm potential of Alternaria alternata, a foliar endophyte of Carica papaya, evidenced by QS assays and in-silico analysis

M, Rashmi.; Meena, Himani; Meena, Chetan Kumar; Kushveer, J. S.; Siddhardha, Busi; Murali, Ayaluru; Sarma, V. Venkateswara

doi:10.1016/j.funbio.2018.07.003

Cited by 28 publications

(17 citation statements)

References 44 publications

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“…After screening the isolates, isolate TP2-6 (an in-house code) was selected for further study. The morphological details of the fungi were observed under stereo-zoom microscope and compound microscope for various characteristics of colony and spore formation ( Mishra et al, 2018 ). For molecular identification, the nuclear ribosomal internal transcribed spacer (ITS) region was amplified by the primers ITS1 (5′-TCC GTA GGT GAA CCT GCG G-3′) and ITS 4 (5′-TCC TCC GCT TAT TGA TAT GC-3′) and sequenced by capillary sequencing, followed by phylogenetic analysis as described in Devadatha et al (2018) .…”

Section: Methodsmentioning

confidence: 99%

“…The effect of sub-MIC concentrations of 2,4-DBP and BAC on the motility, i.e., swimming and swarming ability of P. aeruginosa , has been investigated as described in Mishra et al (2018) . Treatment with BAC acted as a positive control whereas the untreated sample acted as internal control and 2,4-DBP acted as treatment.…”

Section: Methodsmentioning

confidence: 99%

“…The alginate extraction and quantification from the cell-free culture supernatant of P. aeruginosa were performed as described by Rashmi et al (2018) . Concisely, 0.6 mL of boric acid–sulfuric acid (4:1) solution was mixed with 70 μL of cell-free supernatant and vigorously mixed on an ice bath for 10 s. About 20 μL of carbazole solution (0.2% carbazole dissolved in ethanol) was added to the previous mixture, followed by centrifugation (10,000 rpm, 10 min).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we reported that metabolites from endophytic fungi associated with Carica papaya also attenuate in P. aeruginosa ( Mishra et al, 2018 ; Meena et al, 2019 ). Besides being ecologically and physiologically diverse, endophytic fungi are diverse in synthesizing chemically potent and varying secondary metabolites when in association with a medicinally important host ( Rashmi et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa is among the top three gram-negative bacteria according to the WHO's critical priority list of pathogens against which newer antibiotics are urgently needed and considered a global threat due to multiple drug resistance. This situation demands unconventional antimicrobial strategies such as the inhibition of quorum sensing to alleviate the manifestation of classical resistance mechanisms. Here, we report that 2,4-di-tert-butylphenol (2,4-DBP), isolated from an endophytic fungus, Daldinia eschscholtzii, inhibits the quorum-sensing properties of P. aeruginosa. We have found that treating P. aeruginosa with 2,4-DBP substantially reduced the secretion of virulence factors as well as biofilm, and its associated factors that are controlled by quorum sensing, in a dose-dependent manner. Concomitantly, 2,4-DBP also significantly reduced the expression of quorum sensing-related genes, i.e., lasI, lasR, rhlI, and rhlR significantly. Importantly, 2,4-DBP restricted the adhesion and invasion of P. aeruginosa to the A549 lung alveolar carcinoma cells. In addition, bactericidal assay with 2,4-DBP exhibited synergism with ampicillin to kill P. aeruginosa. Furthermore, our computational studies predicted that 2,4-DBP could bind to the P. aeruginosa quorumsensing receptors LasR and RhlR. Collectively, these data suggest that 2,4-DBP can be exploited as a standalone drug or in combination with antibiotic(s) as an anti-virulence and anti-biofilm agent to combat the multidrug resistant P. aeruginosa infection.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

et al. 2020

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“…During antimicrobial screening, the two isolated Alternaria species, CPLE-3 and CPLE-4 exhibited good and moderate activity, respectively against the human pathogenic bacteria tested (Table 2). There is report on the quorum sensing inhibition activity of A. alternata, an endophyte isolated from C. papaya collected in India 9 . The isolated Alternaria species may produce this antibacterial activity by the similar mechanism.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

Lini

Zinnurine

Rahman

et al. 2021

JNR

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Objectives: This work describes the chemical and in-vitro bioactivity studies on endophytic fungi derived from the leaves of Carica papaya L. Methods: Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of in-vitro bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively. Results: A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of Carica papaya. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as Colletotrichum sp., Carvularia sp., and next two as Alternaria sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. Conclusion: Bioactivity and chemical screening of the endophytic fungal extracts of Carica papaya leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

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Symbiotic Relationships with Fungi: From Mutualism to Parasitism

El-Metwally

Mekawey²,

El-Halmouch³

et al. 2023

Plant Mycobiome

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Anti-quorum sensing and antibiofilm potential of Alternaria alternata, a foliar endophyte of Carica papaya, evidenced by QS assays and in-silico analysis

Cited by 28 publications

References 44 publications

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of <i>Carica papaya</i> L. Leaves

Symbiotic Relationships with Fungi: From Mutualism to Parasitism

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