Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy

Liu, Han‐Qing; Wang, Ying‐Ming; Li, Wanfang; Li, Chao; Jiang, Zhihuan; Bao, Jie; Wei, Jinfeng; Jin, Hongtao; Wang, Aiping

doi:10.1159/000484647

Cited by 17 publications

(14 citation statements)

References 32 publications

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“…Another possible advantage of combining phototherapy with a photosensitizer, if applied similarly as in ocular use, is the control of exposed area given that riboflavin is needed for the photodynamic effect. Recently, photodynamic therapy was proposed for treatment of psoriasis [40,41] but, to our knowledge, this is the first study that has suggested a possible use for riboflavin photoactivation for the condition.…”

Section: Discussionmentioning

confidence: 99%

Different photodynamic effects of blue light with and without riboflavin on methicillin-resistant Staphylococcus aureus (MRSA) and human keratinocytes in vitro

2019

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Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of infections in humans. Photodynamic therapy using blue light (450 nm) could possibly be used to reduce MRSA on different human tissue surfaces without killing the human cells. It could be less harmful than 300-400 nm light or common disinfectants. We applied blue light ± riboflavin (RF) to MRSA and keratinocytes, in an in vitro liquid layer model, and compared the effect to elimination using common disinfection fluids. MRSA dilutions (8 × 10 5 /mL) in wells were exposed to blue light (450 nm) ± RF at four separate doses (15, 30, 56, and 84 J/cm 2). Treated samples were cultivated on blood agar plates and the colony forming units (CFU) determined. Adherent human cells were cultivated (1 × 10 4 /mL) and treated in the same way. The cell activity was then measured by Cell Titer Blue assay after 24and 48-h growth. The tested disinfectants were chlorhexidine and hydrogen peroxide. Blue light alone (84 J/cm 2) eliminated 70% of MRSA. This dose and riboflavin eradicated 99-100% of MRSA. Keratinocytes were not affected by blue light alone at any dose. A dose of 30 J/cm 2 in riboflavin solution inactivated keratinocytes completely. Disinfectants inactivated all cells. Blue light alone at 450 nm can eliminate MRSAwithout inactivation of human keratinocytes. Hence, a high dose of blue light could perhaps be used to treat bacterial infections without loss of human skin cells. Photodynamic therapy using riboflavin and blue light should be explored further as it may perhaps be possible to exploit in treatment of skin diseases associated with keratinocyte hyperproliferation.

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Section: Discussionmentioning

confidence: 99%

Different photodynamic effects of blue light with and without riboflavin on methicillin-resistant Staphylococcus aureus (MRSA) and human keratinocytes in vitro

2019

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“…[59]. Although its clinical efficacy, the use of combined therapy using psoralen and UVA (PUVA) [65] showed severe side effects such as nausea, headache, erythema, burns, bringing pain, and discomfort to the patient during the treatment, and consequently causing patient low compliance; besides, there is an increased risk of skin cancer development due to UVA irradiation [66]. Thus, the development of more specific, less toxic, and more bearable new therapeutic approaches is mandatory.…”

Section: Psoriasismentioning

confidence: 99%

“…It was reported that the use of ALA [67,68,69,70], a substrate for the biosynthesis of the photoactive protoporphyrin IX (PPIX), on PDT for the treatment of psoriasis causes apoptosis of the T-cells [67] and a decrease in the production of the main citokines related to the disease development [67]. Another study showed that patients undergoing ALA treatment followed by PDT had diminished lesions and inflammatory response [66]. The reduction in cell proliferation can be related to the activation of the MAPK pathway, promoting an increased level of expression of the apoptosis related genes p38, JNK and ERK, PARP and caspase 3 [66].…”

Section: Psoriasismentioning

confidence: 99%

“…Another study showed that patients undergoing ALA treatment followed by PDT had diminished lesions and inflammatory response [66]. The reduction in cell proliferation can be related to the activation of the MAPK pathway, promoting an increased level of expression of the apoptosis related genes p38, JNK and ERK, PARP and caspase 3 [66]. Other studies have been conducted in order to identify new synthetic PS porphyrins to be applied on the treatment of this skin disorder [71,72].…”

Section: Psoriasismentioning

confidence: 99%

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Chlorin, Phthalocyanine, and Porphyrin Types Derivatives in Phototreatment of Cutaneous Manifestations: A Review

Annunzio

Costa

Mezzina

et al. 2019

IJMS

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Recent scientific research has shown the use of chlorin, phthalocyanines, and porphyrins derivatives as photosensitizers in photodynamic therapy in the treatment of various pathologies, including some of the major skin diseases. Thus, the main goal of this critical review is to catalog the papers that used these photosensitizers in the treatment of acne vulgaris, psoriasis, papillomavirus infections, cutaneous leishmaniasis, and skin rejuvenation, and to explore the photodynamic therapy mechanisms against these conditions alongside their clinical benefits.

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“…Hence, lack of good knowledge of etiopathogenesis impairs the development of an effective treatment. However, recent studies of molecular mechanisms in psoriatic patients give a new promise for effective therapy [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Psoriasis Treatment Changes the Expression Profile of Selected Caspases and their Regulatory MicroRNAs

Wcisło‐Dziadecka¹,

Simka²,

Kaźmierczak³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Psoriasis, an autoimmune diseases of the skin, characterized by patches of abnormal/inflammed skin, although not usually life-threatening, it causes severe discomfort, esthetic impairments, and may lead to impaired social functions and social withdrawal. Besides UV-phototherapy, various anti-inflammatory treatments are applied, depending on the severity of symptoms. In 2008, adalimumab (fully humanized human anti-TNF antibody) was launched for the treatment of psoriasis. In the quest to better understand the pathomechanism of adalimumab’s therapeutic effects, and the acquired resistance to the drug, we have investigated how its administration affect the regulation of the expression of selected caspases, including those activated by inflammosome. Methods: The research was initially carried out on normal human dermal fibroblasts (NHDF) treated with adalimumab for 2, 8 and 24 hours in vitro. Then, expression profile of genes encoding caspases and their regulatory micro-RNAs was determined with the use of oligonucleotide microarray. The validation of the microarray results was carried out by qRT-PCR. The in vitro study was followed by ex-vivo investigation of adalimumab’s effects on the expression of caspase-6 in blood of the psoriatic patients. The samples were collected before, and 2 hours after adalimumab’s administration and the analysis was determined by qRT-PCR. Results: The result of the analysis indicated that introduction of adalimumab to the NHDF culture resulted in the change of the transcription activity of genes encoding caspases and genes encoding miRNAs. The analysis revealed 5 different miRNA molecules regulating the expression of: CASP2, CASP3 and CASP6. There were no statistically significant differences in the expression of gene encoding caspase-6 in the patients’ blood before and 2 hours after the anti-TNF drug administration. Conclusion: We have found that adalimumab administration affects caspases expression, thus they may be used as molecular markers for monitoring the therapy with the use of an anti-TNF drugs, including adalimumab. It is likely that the mechanisms responsible for changed expression profiles of genes encoding caspase-2,-3, and -6, may be caused by the upregulation of the respective microRNA molecules. Increased expression of genes encoding specific caspases may induce inflammatory processes, as well as trigger apoptosis. Furthermore, the proapoptotic activity of caspases may be enhanced by miRNA molecules, which exhibit proapoptotic function. The overexpression of such miRNAs was observed in our study.

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Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy

Cited by 17 publications

References 32 publications

Different photodynamic effects of blue light with and without riboflavin on methicillin-resistant Staphylococcus aureus (MRSA) and human keratinocytes in vitro

Different photodynamic effects of blue light with and without riboflavin on methicillin-resistant Staphylococcus aureus (MRSA) and human keratinocytes in vitro

Chlorin, Phthalocyanine, and Porphyrin Types Derivatives in Phototreatment of Cutaneous Manifestations: A Review

Psoriasis Treatment Changes the Expression Profile of Selected Caspases and their Regulatory MicroRNAs

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