Anti Proliferative Properties of Melissa officinalis in Different Human Cancer Cells

Jahanban-Esfahlan, Akram; Modaeinama, Sina; Abasi, Mozhgan; Abbasi, Mehran Mesgari; Jahanban‐Esfahlan, Rana

doi:10.7314/apjcp.2015.16.14.5703

Cited by 23 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 Some studies have also reported antibacterial, antifungal and antiinflammatory activities, 9 while others have reported antitumoral effects for some Melissa officinalis extracts on some human tumor cell lines. [10][11][12] In order to understand the antitumor activity of this plant, several studies have been conducted to learn more about its composition. M. officinalis is composed of polyphenolic compounds such as caffeic acid derivatives (mostly rosmarinic acid), flavonoids and essential oil.…”

Section: 6mentioning

confidence: 99%

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

et al. 2018

View full text Add to dashboard Cite

Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region. Given our interest in identifying extracts and compounds capable of inhibiting tumor cell growth, and given the antioxidant content and the high consumption of Melissa officinalis in Portugal, this study aimed to test the tumor cell growth inhibitory activity of five different extracts of this plant (aqueous, methanolic, ethanolic, hydromethanolic and hydroethanolic) in three human tumor cell lines: MCF-7, AGS and NCI-H460. All extracts decreased cell growth in all cell lines in a concentration-dependent manner. The ethanolic extract was the most potent one, presenting a GI 50 concentration of approximately 100.9 µg mL −1 in the NCI-H460 lung cancer cells. This extract was characterized by LC-DAD-ESI/MS regarding its phenolic composition, revealing rosmarinic acid as the most abundant compound. The GI 75 concentration of this extract affected the cell cycle profile of these cells. In addition, both the GI 50 and the GI 75 concentrations of the extract induced cellular apoptosis. Moreover, treatment of NCI-H460 cells with this extract caused a decrease in pro-caspase 3 and an increase in p53 levels. This study emphasizes the relevance of the study of natural products as inhibitors of tumor cell growth.

show abstract

Section: 6mentioning

confidence: 99%

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Our results confirm the cytotoxic activity previously reported for lemon balm ethanolic extracts on cancer cells but indicate significant antiproliferative activity at much smaller doses than previously used, in the form of ethanolic extracts. Additionally, we compared the effects on the two different types of breast cancer cells, observing a slightly more significant reduction in cell viability exerted on the estrogen receptor positive MCF-7 cells, as previously reported [7].…”

Section: Cell Viability Effect Against Mcf-10a Breast Epithelialmentioning

confidence: 71%

“…e source of natural compounds investigated here is Melissa officinalis L. (MO), a perennial aromatic plant belonging to the Lamiaceae family, also called lemon balm due to its lemon scent. e chemical composition consists in low amounts of essential oils, triterpenes, and important concentrations of polyphenols (caffeic acid derivatives as rosmarinic acid and flavonoids) [7,8]. Although distributed worldwide, the plant commonly grows in the Mediterranean region and Western Asia, being intensively cultivated in Europe [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Breast cancer, next to lung, prostate, or colon cancer, is among the most investigated types of cancer, particularly in vitro [16,17], but also in vivo [18]. Some studies reported that alcoholic extracts were more effective than hydroalcoholic extracts against certain types of tumor cells, such as the hormone dependent MCF7 breast cancer cells [16], while others highlighted the sensitivity of MCF7 cell line to MO hydroalcoholic extracts [7]; in addition, MO aqueous extracts were proven effective against both hormone-dependent (MCF7 cell line) and non-dependent (MDA-MB-468 and MDA-MB-231 cell lines) breast cancers [18]. More recently, molecular mechanisms were investigated [19], all suggesting the high potential of lemon balm as a valuable source of extracts rich in phytocompounds with benefits against cancer progression.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lemon Balm Extracts Prevent Breast Cancer Progression In Vitro and In Ovo on Chorioallantoic Membrane Assay

Ghiulai

Avram

Stoian

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Breast cancer is the most frequently diagnosed malignant pathology, representing the primary cause of cancer death in women. Natural products are an appealing strategy to limit the progression of the disease. Targeting angiogenesis in breast cancer may positively impact on poor prognosis of breast cancer. As source of natural compounds, we investigated the leaves of Melissa officinalis L. (MO), known as lemon balm, an aromatic plant that spontaneously grows in the South and Western areas of Romania, being traditionally recommended as anxiolytic, antispasmodic, or as digestive remedy. Our aim was to investigate the phytochemical profiling and the antiangiogenic and chemopreventive bioactivity of MO from Banat region, on breast cancer. Two ethanolic extracts of MO (MOE96 and MOE70) and one methanolic extract (MOM80) were subjected to polyphenol and triterpene profiling by HPLC-MS, and the antioxidant capacity was evaluated. The antiangiogenic potential was investigated using the chorioallantoic membrane assay (CAM). The MTT(3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay was used to investigate the cytotoxic effects on MCF-7 and MDA-MB-231breast cancer cells, as well as on MCF-10A normal breast epithelial cells, while apoptosis was performed by DAPI staining. Rosmarinic acid (RA) and ursolic acid (UA) were revealed as dominant phytocompounds. The highest concentration in phytochemicals were found in MOM80; MOE96 was more concentrated in UA, while MOE70 extracted more RA. MOE96 inhibited cancer progression and angiogenesis in the in ovo CAM model using MDA-MB-231 cells, inhibiting breast cancer progression and angiogenesis for the MDA-MB-231 breast cancer cell line; no secondary tumoral areas were registered, indicative for a preventive effect against breast tumor cell invasiveness. The highest cell inhibitory activity was also exhibited by MOE96, in particular against the estrogen receptor positive MCF7 breast cancer cell line, with no cytotoxic effect on healthy cells. The estrogen receptor positive MCF7 cell line proved to be more sensitive to the extract antiproliferative activity than the triple negative MDA-MB-231 breast cancer cell line. Nevertheless, the chemopreventive potential of MOE96 extract is phenotype-dependent and is rather related to the apoptosis and antiangiogenic effects suggesting a multitargeted mechanism of action due to its multiple compound composition next to a concentration ratio of RA : UA in favor of UA.

show abstract

“…Numerous specific biochemical activities have been reported 17 such as acetylcholinesterase inhibition and antioxidant activity 18 and its use for the treatment of cancer 19,20 and of diabetes 21 have been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Composition of Volatile Oil and Methanolic Extract of Jordanian Melissa Officinals L. and Actions Againsthuman Cancer Cell Lines

Barakat¹,

Hudaib²,

EL-asadand³

et al. 2016

Orient. J. Chem

View full text Add to dashboard Cite

show abstract

Anti Proliferative Properties of Melissa officinalis in Different Human Cancer Cells

Abstract: manner, suggesting that an optimal biological dose is more important than a maximally tolerated one. Moreover, the antiprolifreative effect of MO seems to be tumor type specific, as hormone dependant cancers were more sensitive to antitumoral effects of MOE.

Cited by 23 publications

References 20 publications

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

Lemon Balm Extracts Prevent Breast Cancer Progression In Vitro and In Ovo on Chorioallantoic Membrane Assay

Composition of Volatile Oil and Methanolic Extract of Jordanian Melissa Officinals L. and Actions Againsthuman Cancer Cell Lines

Contact Info

Product

Resources

About