Anti-proliferative Efficiency of Pulsed Electric Field Treated <i>Curcuma Longa</i> (Turmeric) Extracts on Breast Cancer Cell Lines

Poompavai, S.; Sree, V. Gowri

doi:10.1080/03772063.2020.1799873

Cited by 14 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the total phenol content of the extract is calculated in terms of Gallic Acid Equivalent (GAE), (2) where C is the total phenolic compound in mg/g. of extract, c is the concentration of Gallic acid from the calibration curve, V is the volume of the extract in ml, m is the weight of extracts in g. The effect of pulsed electric fields on the total phenol content of each extract is observed [13,15,16].…”

Section: Total Phenol Contentsmentioning

confidence: 99%

“…Microplate reader spectrophotometers are used to measure the absorbance at 517 nm (color changes from deep violet to light yellow). Samples are measured in three replicates and the percentage inhibition of DPPH is calculated by the formula given below [13,15,16].…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…These cells are cultured as a monolayer in Dulbecco's modified Eagle's medium (DMEM) with 10% FBS, penicillin (100U/ml), and streptomycin (100μg/ml) in a humidified atmosphere of 50μg/ml CO2 at 37°C. [15][16][17].…”

Section: Cell Culture and Cell Viability Assaymentioning

confidence: 99%

“…Phenol contents have decreased by 6.36% between 2% and 3% combinations. In our previous works [15][16][17]23], we have analyzed the phenol contents and antioxidant activity of individual extracts, when compared with them, the obtained poly-herbal mixtures have exhibited higher phenols and antioxidant activity than individual extracts, which may be due to the synergistic effect of both the extracts. Turmeric and its combinations (turmeric with pepper) have exhibited the highest phenol content and antioxidant activity than other combinations.…”

Section:  Vinca Rosea and Its Combinationmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Pulsed Electric Fields on Enhanced Extraction and Cytotoxicity of Poly-Herbal Mixtures Against Estrogen Receptor-Positive Breast Cancer Cell Lines

Sadasivam

Varadarajan

2021

Preprint

View full text Add to dashboard Cite

The mixture of different bioactive constituents from plant extracts and several other natural products produces synergistic effects. This synergistic activity aids in treating and preventing various diseases by reducing the usage of conventional drugs. The present investigation aimed to study the effect of poly-herbal formulations of selected plants (Turmeric, Pepper, Neem, Vinca rosea ) on improved biological activity with and without pulsed electric fields(PEF). Selected plant extracts were combined in different proportions(1% to 3%) to formulate a poly-herbal mixture. Ethanolic crude extracts were extracted by Soxhlet method and their phenolic activity and antioxidant activity were quantified. Further, to enhance the recovery of intracellular components, electrical fields of different parameters were used. The effect of PEF on formulated mixtures was compared with untreated extracts, which exhibited higher phenolic and antioxidant activity than untreated extracts. Out of all combinations, turmeric with pepper showed higher phenolic and antioxidant activity, and hence, these combinations were checked for their potential cytotoxicity against MCF-7 Estrogen breast cancer cells. In addition, different electrical ((EP1)High intensity,short duration & (EP2)Low intensity,long duration) pulses were used to enhance the uptake of extracts into the cell membrane. Lowest cell viabilities of 19.41%(raw extract),14.75%(pulsed extract),13.28%(pulsed extract+EP1) and 9.982%(Pulsed extract+EP2) after 72hrs of treatment for 1000μg/ml concentration of turmeric(97%) and pepper(3%) mixture was observed. This shows the potential of the synergistic effect of electrical pulses and poly-herbal mixture, achieving phenolic and antioxidant-rich extracts causing higher cell death. This technique can be used as an effective method to treat breast cancer avoiding undesirable side effects due to higher doses of synthetic drugs and single herb.

show abstract

Section: Total Phenol Contentsmentioning

confidence: 99%

Section: Antioxidant Activitymentioning

confidence: 99%

Section: Cell Culture and Cell Viability Assaymentioning

confidence: 99%

Section:  Vinca Rosea and Its Combinationmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Pulsed Electric Fields on Enhanced Extraction and Cytotoxicity of Poly-Herbal Mixtures Against Estrogen Receptor-Positive Breast Cancer Cell Lines

Sadasivam

Varadarajan

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, the pulsed electric field and enzyme-assisted extraction techniques can be used as preparatory or adjuvant techniques in an antioxidant extraction process and they can be combined with conventional and non-conventional techniques of extraction. Moreover, the use of these extraction techniques, in addition to being ecological and efficient, allows the extraction of active molecules with lower structural modification and great efficiency for use in various health treatments associated with oxidative stress [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Environmentally Friendly Techniques and Their Comparison in the Extraction of Natural Antioxidants from Green Tea, Rosemary, Clove, and Oregano

Calderón‐Oliver

Ponce‐Alquicira

2021

Molecules

View full text Add to dashboard Cite

Many current food and health trends demand the use of more ecological, sustainable, and environmentally friendly techniques for the extraction of bioactive compounds, including antioxidants. However, extraction yields and final antioxidant activities vary between sources and are highly influenced by the given extraction method and nature and ratio of the employed solvent, especially for total polyphenols, flavonoids, and anthocyanins, which are well recognized as natural antioxidants with food applications. This review focused on the most common extraction techniques and potential antioxidant activity in the food industry for various natural antioxidant sources, such as green tea, rosemary, clove, and oregano. Green extraction techniques have been proven to be far more efficient, environmentally friendly, and economical. In general, these techniques include the use of microwaves, ultrasound, high hydrostatic pressure, pulsed electric fields, enzymes, and deep eutectic solvents, among others. These extraction methods are described here, including their advantages, disadvantages, and applications.

show abstract

Pulsed Electric Fields as a Green Technology for the Extraction of Bioactive Compounds

Theagarajan,

Balendran,

Sethupathy

2024

Methods and Protocols in Food Science

View full text Add to dashboard Cite

Anti-proliferative Efficiency of Pulsed Electric Field Treated Curcuma Longa (Turmeric) Extracts on Breast Cancer Cell Lines

Cited by 14 publications

References 42 publications

Effect of Pulsed Electric Fields on Enhanced Extraction and Cytotoxicity of Poly-Herbal Mixtures Against Estrogen Receptor-Positive Breast Cancer Cell Lines

Effect of Pulsed Electric Fields on Enhanced Extraction and Cytotoxicity of Poly-Herbal Mixtures Against Estrogen Receptor-Positive Breast Cancer Cell Lines

Environmentally Friendly Techniques and Their Comparison in the Extraction of Natural Antioxidants from Green Tea, Rosemary, Clove, and Oregano

Pulsed Electric Fields as a Green Technology for the Extraction of Bioactive Compounds

Contact Info

Product

Resources

About