Anti-Proliferative Effects of Siegesbeckia orientalis Ethanol Extract on Human Endometrial RL-95 Cancer Cells

Abstract

Endometrial cancer is a common malignancy of the female genital tract. This study demonstrates that Siegesbeckia orientalis ethanol extract (SOE) significantly inhibited the proliferation of RL95-2 human endometrial cancer cells. Treating RL95-2 cells with SOE caused cell arrest in the G2/M phase and induced apoptosis of RL95-2 cells by up-regulating Bad, Bak and Bax protein expression and down-regulation of Bcl-2 and Bcl-xL protein expression. Treatment with SOE increased protein expression of caspase-3, -8 and -9 dose-dependently, indicating that apoptosis was through the intrinsic and extrinsic apoptotic pathways. Moreover, SOE was also effective against A549 (lung cancer), Hep G2 (hepatoma), FaDu (pharynx squamous cancer), MDA-MB-231 (breast cancer), and especially on LNCaP (prostate cancer) cell lines. In total, 10 constituents of SOE were identified by Gas chromatography-mass analysis. Caryophyllene oxide and caryophyllene OPEN ACCESSMolecules 2014, 19 19981 are largely responsible for most cytotoxic activity of SOE against RL95-2 cells. Overall, this study suggests that SOE is a promising anticancer agent for treating endometrial cancer.

Comparative Analysis of Chemical Composition, Anti-Inflammatory Activity and Antitumor Activity in Essential Oils from Siegesbeckia orientalis, S. glabrescens and S. pubescens with an ITS Sequence Analysis

“…A recent study [24] showed that SO ethanol extract significantly inhibited the proliferation of RL 95-2 human endometrial cancer cells. Furthermore, SO ethanol extract was effective against A549 (lung cancer), HepG2 (hepatpma), MDA-MB-231 (breast cancer), and especially on LNCaP (prostate cancer) cell lines.…”

Bulnesia sarmientoi Supercritical Fluid Extract Exhibits Necroptotic Effects and Anti-Metastatic Activity on Lung Cancer Cells

“…It has been demonstrated that (−)-guaiol possesses significant cytotoxicity on NSCLC cells and regulates autophagic cell death [49,50]. β-Caryophyllene, an important component in the extracts of various species of medicinal plants, has been reported to have anti-proliferative effects on various cancer cells, including pancreatic cancer, colon cancer [51], hepatoma [52], endometrial cancer [53], and skin epidermoid cancer [54].…”

Differential Cytotoxic Activity of Essential Oil of Lippia citriodora from Different Regions in Morocco

“…Furthermore, other studies have mentioned that citrals were apoptotic against chronic lymphoid leukemia by activation of caspase-3. [15] In addition, other authors showed that b-elemene inhibits the proliferation of human glioblastoma cells through the activation of glia maturation factor b and induces sensitization to cisplatin. [15] In addition, other authors showed that b-elemene inhibits the proliferation of human glioblastoma cells through the activation of glia maturation factor b and induces sensitization to cisplatin.…”

“…[14] In the same idea, caryophyllene oxide has been reported to be responsible for a cytotoxic activity against RL95-2 cells of endometrial cancer. [15] In addition, other authors showed that b-elemene inhibits the proliferation of human glioblastoma cells through the activation of glia maturation factor b and induces sensitization to cisplatin. [16] A high cytotoxic activity of b-elemene on MCF7 tumor cells with IC 50 = 14.70 lg/ml was also reported by Chen et al [17] These results also corroborate those reporting that limonene is an inhibitor of tumor progression by acting on the protein P21ras.…”