Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer

Lee, Dong Gyu; Jang, Sung-Il; Kim, Young-Rang; Yang, Kyeong Eun; Yoon, So Jung; Lee, Zee-Won; An, Hyun Joo; Jang, Ik‐Soon; Choi, Jong‐Soon; Yoo, Hwa‐Seung

doi:10.1007/s11655-014-1789-8

Cited by 30 publications

(23 citation statements)

References 25 publications

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“…Ginseng extract contains characteristic ringstructure compounds known as ginsenosides, many literature reported that ginsenosides have the effect for anti-tumor. (5)(6)(7) Since 1992, we have extracted and isolated total saponins of panax ginseng (TSPG) from ginseng herb, containing multiple ginsenosides, (8)(9)(10)(11)(12) further purified TSPG using macroporous resin to yield a fraction, termed panaxadiol saponins component (PDS-C) which contains 5 ginsenosides. PDS-C was formulated into capsule named as Pai-Neng-Da (派能达), for which we have been granted a patent by China Food and Drug Administration (CFDA) in 2010 to treat pancytopenia from various causes.…”

mentioning

confidence: 99%

Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

Sun

Zhao

Qian

et al. 2017

Chin. J. Integr. Med.

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mentioning

confidence: 99%

Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

Sun

Zhao

Qian

et al. 2017

Chin. J. Integr. Med.

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“…In a recent study, it was demonstrated that butanolic extract of mountain ginseng had anti-proliferative and pro-apoptotic effects on A549 human lung cancer cells via modulation of the expression and/or activity of p53, caspase-3 and nuclear factor (NF)-κB (8). Furthermore, a previous study by our group provided evidence that Rb1, Rb2 and Rg1, three of the most abundant ginsenosides in the ginseng extract, induced apoptosis in A549 cells through extrinsic (death receptor-mediated) apoptotic pathways (9). In addition, there is clinical evidence that red ginseng powder effectively enhances postoperative immunity and survival in patients with stage III gastric cancer (10), suggesting the potential clinical applications of ginseng preparations against gastric cancer.…”

Section: An Enzymatically Fortified Ginseng Extract Inhibits Prolifermentioning

confidence: 78%

“…Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; p-mTOR, phosphorylated mammalian target of rapamycin; PKB, protein kinase B; IκBα, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α; GBX, ginseng butanol extract; FGX, fortified ginseng extract. quantities of Rb1, inhibits growth and induces apoptosis of A549 human lung cancer cells (9), implying a positive role of Rb1 on the GBX-induced anti-growth and pro-apoptotic effects. In the present study, using HPLC-UV analysis, it was demonstrated that five ginsenosides, including Rh1, Rb1, Rg3(R), compound K and Rh2, were enriched in FGX compared with the ginsenoside profile in GBX.…”

Section: Discussionmentioning

confidence: 97%

An enzymatically fortified ginseng extract inhibits proliferation and induces apoptosis of KATO3 human gastric cancer cells via modulation of Bax, mTOR, PKB and IκBα

Hwang

Baek

Jang

et al. 2014

Molecular Medicine Reports

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Accumulative evidence suggests ginseng extract and/or its major components, ginsenosides and compound K, a metabolized ginseng saponin, have anti-cancer effects. In the present study, the effects of a ginseng butanolic extract (GBX) and an enzymatically fortified ginseng extract (FGX), with enriched ginsenosides and compound K, on the growth of KATO3 human gastric cancer cells were investigated using a cell viability assay. While treatment with GBX at 31.25-125 mg/ml for 24 h did not affect the proliferation of KATO3 cells, FGX under the same conditions inhibited cell proliferation in a concentration-dependent manner. Furthermore, Annexin V/PI-staining and flow cytometric analysis demonstrated that the population of apoptotic KATO3 cells was increased following treatment with FGX, which was greater than in the GBX-treated cells, suggesting that FGX had a stronger apoptotic effect than GBX. To investigate the underlying mechanism of the cytostatic and cytotoxic effects of the ginseng extracts, apoptosis-associated proteins were assessed using western blot analysis. The data revealed higher expression levels of B-cell lymphoma 2-associated X protein (Bax), lower expression of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) and reduced phosphorylation of mammalian target of rapamycin (mTOR) and protein kinase B (PKB) in the FGX-treated KATO3 cells than in the GBX-treated cells. Collectively, these results demonstrated for the first time, to the best of our knowledge, that FGX had stronger anti-proliferative and pro-apoptotic effects on KATO3 cells than GBX. The anti-proliferative and/or pro-apoptotic effects of FGX appeared to be mediated via the upregulation of Bax, IκBα proteolysis (activation of nuclear factor-κB) and the blocking of mTOR and PKB signals.

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“…A total of 7 ginsenosides, including Rg1, Re, Rf, Rb1, Rc, Rb2 and F1, were present in the GBX. By contrast, 5 ginsenosides, including Rh1, Rg3(S), Rg3(R), compound K and Rh2, were exclusively detected in MRGX (6,10 Cell viability assay. Cells (5x10 3 /well) were placed in a 96-well plate.…”

Section: Methodsmentioning

confidence: 88%

Modified Panax ginseng extract regulates autophagy by AMPK signaling in A549 human lung cancer cells

Yoo

Kim²,

et al. 2017

Oncology Reports

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Abstract. Panax ginseng has been used worldwide as a traditional medicine for the treatment of cancer and other diseases. The antiproliferative activity of ginseng has been increased after enzymatic processing of ginseng saponin, which may result in the accumulation of minor saponins, such as Rh2, Rg3, compound K and protopanaxatriol type (PPT) in modified regular ginseng extract (MRGX). In the present study, the anticancer activity and the associated mechanisms of MRGX were investigated using A549 human lung cancer cells. To elucidate the mechanisms underlying the effects of MRGX, we performed a microarray analysis of gene expression in the A549 cells. Molecular mechanisms that were associated with the anticancer activity of MRGX were studied, with a special focus on the autophagy-related multiple signaling pathways in lung cancer cells. Microarray analyses elucidated autophagyrelated genes affected by MRGX. Administration of MRGX at 100 µg/ml induced punctate cytoplasmic expression of LC3, Beclin-1 and ATG5 and increased expression of endogenous LC3-II whereas 50 µg/ml did not inhibit the proliferation of A549 cells. Compared to the control cells, in cells treated with MRGX at 100 µg/ml, the level of p-Akt was increased, while that of mTOR-4EBP1 was decreased. Downregulation of mTOR and 4EBP1 in the MRGX-treated cells was found not to be a p-Ulk (S757)-dependent pathway, but a p-Ulk (S317)-dependent autophagic pathway, using AMPK. These data suggest that MRGX regulates AMPK and induces autophagy in lung cancer cells.

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Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer

Abstract: Major ginsenosides such as Rb1, Rb2 and Rg1 comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.

Cited by 30 publications

References 25 publications

Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

An enzymatically fortified ginseng extract inhibits proliferation and induces apoptosis of KATO3 human gastric cancer cells via modulation of Bax, mTOR, PKB and IκBα

Modified Panax ginseng extract regulates autophagy by AMPK signaling in A549 human lung cancer cells

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