Anti-proliferative effect of main dietary phytosterols and β-cryptoxanthin alone or combined in human colon cancer Caco-2 cells through cytosolic Ca+2 – and oxidative stress-induced apoptosis

Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

“…In a cohort study, 0.70-0.87 µM of β-cryptoxanthin was recorded in the serum from the Japanese population, which may substantially increase up to 4.12 µΜ after supplementation with βcryptoxanthin-rich diet (e.g., Satsuma mandarin juice 125 mL/day for 12 weeks). Earlier studies showed that the β-cryptoxanthin treatments of 5-20 µΜ can significantly reduce the multiplication of premalignant A549 and malignant lung cancer BEAS-2B cells [20], stomach tumor BGC-823 cells [18], and human colon adenocarcinoma Caco-2 cells [19]. Similarly, in a comparative study of the anticancer potential of 15 kinds of carotenoids, neoxanthin, fucoxanthin, phytofluene, ζ-carotene, and lycopene at 5-20 µM concentrations showed cytotoxicity against LNCaP, PC-3, DU 145 cells.…”

“…To the best of our knowledge, no comprehensive studies were available on the effect of provitamin A carotenoids β-cryptoxanthin on cervical cancer. However, several epidemiological in vitro and in vivo studies have demonstrated the protective role of β-cryptoxanthin in several types of cancer, including lung [15][16][17], stomach [18], and human colon adenocarcinoma [19]. In mechanistic studies, β-cryptoxanthin triggered antitumor activities were largely modulated by significantly enhanced production and accumulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) [19], decrease in cell viability, cell cycle arrest by downregulations of cell cycle proteins cyclin D1 and cyclin E [20], increased activities of p21 cyclin-dependent kinase inhibitor and retinoic acid receptor (RAR)-β [18], activation of Bcl-2-associated death promoter (Bad) protein, suppressed cell migration, and caspase-triggered apoptosis [19].…”

Characterization of sterolic and alcoholic fractions of some Algerian olive oils according to the variety and ripening stage

“…It is composed essentially of triacylglycerols which represent 98% of the oil and minor components such as tocopherols, phenolic compounds, alcohols and phytosterols (Bianchi, 2003). It has been demonstrated that the unsaponifiable fraction presented a beneficial therapeutic effect for human health (Abuajah et al, 2015;Cilla et al, 2015;Mahaddalkar et al, 2015).…”

Exemplified screening standardization of potent antioxidant nutraceuticals by principles of design of experiments

“…6, the results of DOE are reliable as they are based on statistical basis. At present, functional food based formulations are subjected for therapeutic evaluation, in order to investigate their additive or synergistic effects (Cilla, Attanzio, Barberá, Tesoriere, & Livrea, 2015;Patel, 2015). The chances of systematic and random errors are high in case of OVAT studies which may result in false positive or negative outcome.…”