Anti-proliferative, apoptotic induction, and anti-migration effects of hemi-synthetic 1&amp;prime;&lt;em&gt;S&lt;/em&gt;-1&amp;prime;-acetoxychavicol acetate analogs on MDA-MB-231 breast cancer cells

Liew, Su Ki; Azmi, Mohamad Nurul; In, Lionel Lian Aun; Awang, Khalijah; Nagoor, Noor Hasima

doi:10.2147/dddt.s130349

Cited by 15 publications

(13 citation statements)

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“…Increased proliferative capacity, decreased apoptosis, and enhanced invasion and migration are common biological features of tumor cells and the basis for tumor growth, metas tasis, and recurrence [26,27]. To further investigate the function of miR-103a-3p in thyroid cancer, we transfected the thyroid cancer cell line BHT101 with si-miR-103a-3p.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

Zhang¹,

Sun²,

Wu³

et al. 2021

neo

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MicroRNA (miR)-103a-3p has been shown to be involved in the development and progression of several types of cancer. However, the role of miR-103a-3p in thyroid cancer remains unclear. This study investigated the effects of miR-103a-3p on the biological characteristics of thyroid cancer cells and related mechanisms. In the present study, we found that the expression of miR-103a-3p was increased in thyroid cancer tissues compared to that in non-cancerous tissues. Additionally, the expression of miR-103a-3p in thyroid cancer cell lines (TPC-1, SW579, BHT101, K1) was markedly higher than that in the human thyroid cell line (Nthy-ori3-1). Silencing of miR-103a-3p obviously inhibited proliferation, migration, and invasion and promoted apoptosis of BHT101 cells. miR-103a-3p upregulation promoted the proliferation, migration, and invasion and inhibited apoptosis of K1 cells. Mechanistically, LATS1was identified as a functional target of miR-103a-3p and miR-103a-3p negatively regulated LATS1 expression. miR-103a-3p knockdown (or upregulation) partially reversed the effects of LATS1 knockdown (or overexpression) on proliferation, apoptosis, migration, and invasion of thyroid cancer cells. LATS1 knockdown inhibited the phosphorylation of YAP in BHT101 cells and pro moted the nuclear translocation of YAP. Whereas, miR-103a-3p downregulation reversed the inhibitory effect of LATS1 knockdown on Hippo signaling pathway. Moreover, overexpression of LATS1 induced YAP phospho rylation in K1 cells and inhibits nuclear translocation of YAP, and the upregulation of miR-103a-3p reversed this effect. Knockdown of miR-103a-3p inhibited tumor growth and progression in vivo. Taken together, knockdown of miR-103a-3p inhibits proliferation, migration, and invasion and promotes apoptosis of thyroid cancer cells through the Hippo signaling pathway by upregulating LATS1.

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Section: Discussionmentioning

confidence: 99%

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

Zhang¹,

Sun²,

Wu³

et al. 2021

neo

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“…Thus, this hydroxyl group may not be a necessary component for these activities. In structure-activity relationship (SAR) studies of the compounds with similar skeletal structure, phenylpropanoids, the substitution of acetoxyl group at 4′ and 1 positions of phenylpropanoids (adjusted nomenclature similar to our study) are required for the strong inhibitory effects on β-hexosaminidase released from RBL-2H3 [31] and cytotoxic effects against MDA-MB-231 breast cancer cells [32]. For the inhibitory activity of NO production, the methoxyl group at 4′ - position and the hydroxyl group at 3- position with one glucopyranosyl moiety in the molecule are required [33].…”

Section: Discussionmentioning

confidence: 99%

Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Pipatrattanaseree

Itharat

Mukkasombut

et al. 2019

BMC Complement Altern Med

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BackgroundThe root of Baliospermum montanum has been used as an ingredient of traditional Thai medicines for the treatments of several diseases including itching eczema, muscle and joint inflammation, and cancer. Few studies have been done on phytochemical components of this root. In this study, we isolated major compounds of the crude ethanolic extract of B. montanum root and developed and validated a high performance liquid chromatographic (HPLC) method for the determination of its major components. We then investigated anti-allergic, anti-inflammatory and cytotoxic activities of the extract.MethodsThe aims of this study were to investigate in vitro activities including inhibitory effect of β-hexosaminidase released from RBL-2H3 cells, inhibition of nitric oxide (NO) production from RAW 264.7 cells and cytotoxic activity against cancerous liver cell lines (HepG2 and KKU M156) by using sulforhodamine B (SRB) assay. Isolation of major components was conducted by using column chromatographic method. Isolated major compounds were analyzed by using high performance liquid chromatography (HPLC).ResultsThe crude extract exhibited the highest cytotoxic activity, with IC50 less than 1 μg/mL, while its anti-allergy and anti-inflammation were also potent with IC50 less than 6 μg/mL. Three propiophenones isolated from B. montanum root exhibited moderate cytotoxic activities (IC50 > 20 μg/mL). Two of the propiophenones found were major components that can be detected by HPLC. The developed and validated HPLC method showed good accuracy, precision, and linearity.ConclusionThe results of this study suggested that ethanolic extract of of B.montanum root can be a potential source of anti-allergy, anti-inflammation, and anti-cancer compounds. The isolated compounds can serve as markers when B. montanum is used in herbal remedies but not as overall responsive markers. The HPLC method developed may be useful for quality control in the production of the extract and for further formulation developments. However, investigation of several associated biological activities is necessary before the development can proceed further. Minor active compounds should be isolated and a more sensitive analytical method should be developed to detail the key responsive components of the ethanolic extract of B. montanum root.Electronic supplementary materialThe online version of this article (10.1186/s12906-019-2449-0) contains supplementary material, which is available to authorized users.

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“…Migration and invasion are the most important biological characteristics of malignant tumors and are the 2 main causes of death in tumor patients [ 16 ]. Migration and invasion of TNBC have been previously studied [ 26 , 27 ]. One study showed that the abilities of growth and invasion are enhanced in breast cancer cells with high expression of α1-AT [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells

Zhao

Mao

et al. 2018

Med Sci Monit

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Backgroundα1-antitrypsin (α1-AT) is highly expressed in many tumors. However, to the best of our knowledge, its relationship to triple negative breast cancer (TNBC) has not yet been studied. Thus, in this research we first explored the influence of α1-AT silencing on the abilities of migration and invasion, and then further study its molecular mechanism in TNBC cells.Material/MethodsThe viability of MDA-MB-231 cells were detected using cell counting kit-8 (CCK-8). The abilities of migration and invasion were examined by Transwell assay. The metastasis-related factors were tested respectively by quantitative real-time PCR (qRT-PCR) and western blot assays.ResultsOur study results showed that α1-AT level in TNBC tissues was higher than non-triple negative breast cancer (n-TNBC) and adjacent normal breast tissues. The high expression of α1-AT was linked to type of cancer, tumor size, TNM stage and metastasis, but was not correlated with α1-AT expression and age. si-α1-AT suppressed the viability, migration, and invasion of cells. While si-α1-AT upregulated E-cadherin and the tissue inhibitor of metalloproteinases-2 (TIMP-2) levels, it downregulated metastasis associated 1 (MTA1), matrix metallopeptidase 2 (MMP2), phosphorylated-mammalian target of rapamycin (p-mTOR), phosphorylated-protein kinase B (p-Akt), and phosphorylated-phosphatidylinositol 3 kinase (p-PI3K) levels. We also found that the PI3K/Akt/mTOR pathway activator reversed the role of si-α1-AT in metastasis-related factors.Conclusionsα1-AT was highly expressed in TNBC tissues, and its silencing suppressed the abilities of migration and invasion in TNBC cells and downregulated the PI3K/Akt/mTOR pathway. Thus, α1-AT may have a potential therapeutic effect on TNBC.

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Anti-proliferative, apoptotic induction, and anti-migration effects of hemi-synthetic 1′<em>S</em>-1′-acetoxychavicol acetate analogs on MDA-MB-231 breast cancer cells

Cited by 15 publications

References 48 publications

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells

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