Anti-proliferation Effects of Interferon-gamma on Gastric Cancer Cells

Abstract

IFN-γ plays an indirect anti-cancer role through the immune system but may have direct negative effects on cancer cells. It regulates the viability of gastric cancer cells, so we examined whether it affects their proliferation and how that might be brought about. We exposed AGS, HGC-27 and GES-1 gastric cancer cell lines to IFN-γ and found significantly reduced colony formation ability. Flow cytometry revealed no effect of IFN-γ on apoptosis of cell lines and no effect on cell aging as assessed by β-gal staining. Microarray assay revealed that IFN-γ changed the mRNA expression of genes related to the cell cycle and cell proliferation and migration, as well as chemokines and chemokine receptors, and immunity-related genes. Finally, flow cytometry revealed that IFN-γ arrested the cells in the G1/S phase. IFN-γ may slow proliferation of some gastric cancer cells by affecting the cell cycle to play a negative role in the development of gastric cancer.

Interferon Apha 2b for Treating Patients with JAK2V617F Positive Polycythemia Vera and Essential Thrombocytosis

“…2 promoting cell apoptosis. IFNs could cause structural change of cell membrane and cytoplasm, DNA fragmentation and formation of apoptotic body; IFNs could upregulate TRAIL and FasL, and after conbined with corresponding receptor, is able to activate death domain structure that is associated with Fas, and induce apoptosis by activating downstream proteases, eg., Caspase -4 and Caspase -8 (Chawla-Sarkar et al, 2003;Guo et al, 2012;Dirican et al, 2013;Zhao et al, 2013;Laljee et al, 2013). 3 modulating immunological function.…”

Local Cervical Immunity in Women with Low-grade Squamous Intraepithelial Lesions and Immune Responses After Abrasion

“…A study of gastric cancer suggested that IFN-γ might slow proliferation of some gastric cancer cells by affecting the cell cycle to play a negative role in the development of gastric cancer (Zhao et al, 2013). Corresponding to the present study, at 6 months after abrasion by both Pap smear and cryotherapy showed significant increased (p<0.001) IFN-γ and TNF-α mRNA expression, whereas the secreted IL-10 levels significantly decreased (p<0.001) in comparison with the base line at first recruitment as in Figure 5.…”

Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells

“…In addition, recent studies also have indicated that IFN-γ has the ability to directly inhibit cancer cell growth (Tate et al, 2012;Liu et al, 2012;Jin et al, 2013;Zhao et al, 2013;Wang, 2014); however, the mechanism remains unclear. Our present study demonstrated that the FADD/caspase 8/tBid/mitochondria pathway plays an important role in IFN-γ-induced apoptosis in the NSCLC cell lines H322M and H226.…”

“…IFN-γ is a major activator of various types of immune cells (Wakita et al, 2009;Hayakawa et al, 2011) and also can induce the expression of the major histocompatibility complex in both normal and malignant cells and provokes inflammatory 1 infiltrate infiltration (Chen et al, 2012;Hong et al, 2014). In addition, IFN-γ can directly inhibit the growth of cells, in some cases, through the induction of cell cycle arrest with or without the concomitant induction of apoptosis (Tate et al, 2012;Liu et al, 2012;Jin et al, 2013;Zhao et al, 2013Wang, 2014. Recognition of the direct antiproliferative properties of IFN-γ and its ability to stimulate the immune system has led to the investigation of its therapeutic activity in a number of malignant conditions.…”