Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts

Neves, Dina; Caetano, P.; Oliveira, Joana; Maia, Cristiana; Horta, M.; Sousa, Nelson; Salgado, Marco; Dionísio, Lídia; Magan, Naresh; Cravador, A.

doi:10.1007/s10658-013-0357-6

Cited by 14 publications

(14 citation statements)

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“…A significant reduction of the root rot disease symptoms in Q. suber has been observed when P. purpurea is growing in the tree surroundings which accounts for the importance of the triangular interactions between Phlomis purpurea, Phytophthora cinnamomi and Q. suber/Q. ilex previously reported by Neves et al [ 5 ] and Neves [ 6 ]. A novel triterpenoid (phlomispurpentaolone), recently isolated from P. purpurea roots and exuded to the rhyzosphere, exhibits anti- Phytophthora and antitumor activities [ 7 ].…”

Section: Introductionmentioning

confidence: 80%

“…A survey of the P. cinnamomi infested habitats in Algarve, including cork oak stands, led to the discovery of some plant species not infected by this oomycete [ 4 ]. Among them Phlomis purpurea proved to be not only host for P. cinnamomi [ 5 ] but also and noticeably to inhibit the pathogen hyphae to penetrate beyond the surface layer of the root epidermis [ 6 ]. Moreover, P. purpurea root extracts reduce the production of P. cinnamomi disease cycle structures and prevent germination of chlamydospores and zoospores, suggesting the ability of P. purpurea to reduce Q. suber root infection by P. cinnamomi , thus having the potential to reduce disease spread [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among them Phlomis purpurea proved to be not only host for P. cinnamomi [ 5 ] but also and noticeably to inhibit the pathogen hyphae to penetrate beyond the surface layer of the root epidermis [ 6 ]. Moreover, P. purpurea root extracts reduce the production of P. cinnamomi disease cycle structures and prevent germination of chlamydospores and zoospores, suggesting the ability of P. purpurea to reduce Q. suber root infection by P. cinnamomi , thus having the potential to reduce disease spread [ 5 ]. Phlomis purpurea plants grown in glasshouses reduce growth of the soil pathogen inoculum.…”

Section: Introductionmentioning

confidence: 99%

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De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi

et al. 2017

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Background Phlomis plants are a source of biological active substances with potential applications in the control of phytopathogens. Phlomis purpurea (Lamiaceae) is autochthonous of southern Iberian Peninsula and Morocco and was found to be resistant to Phytophthora cinnamomi. Phlomis purpurea has revealed antagonistic effect in the rhizosphere of Quercus suber and Q. ilex against P. cinnamomi. Phlomis purpurea roots produce bioactive compounds exhibiting antitumor and anti-Phytophthora activities with potential to protect susceptible plants. Although these important capacities of P. purpurea have been demonstrated, there is no transcriptomic or genomic information available in public databases that could bring insights on the genes underlying this anti-oomycete activity.ResultsUsing Illumina technology we obtained a de novo assembly of P. purpurea transcriptome and differential transcript abundance to identify putative defence related genes in challenged versus non-challenged plants. A total of 1,272,600,000 reads from 18 cDNA libraries were merged and assembled into 215,739 transcript contigs. BLASTX alignment to Nr NCBI database identified 124,386 unique annotated transcripts (57.7%) with significant hits. Functional annotation identified 83,550 out of 124,386 unique transcripts, which were mapped to 141 pathways.39% of unigenes were assigned GO terms. Their functions cover biological processes, cellular component and molecular functions. Genes associated with response to stimuli, cellular and primary metabolic processes, catalytic and transporter functions were among those identified.Differential transcript abundance analysis using DESeq revealed significant differences among libraries depending on post-challenge times.Comparative cyto-histological studies of P. purpurea roots challenged with P. cinnamomi zoospores and controls revealed specific morphological features (exodermal strips and epi-cuticular layer), that may provide a constitutive efficient barrier against pathogen penetration. Genes involved in cutin biosynthesis and in exodermal Casparian strips formation were up-regulated.ConclusionsThe de novo assembly of transcriptome using short reads for a non-model plant, P. purpurea, revealed many unique transcripts useful for further gene expression, biological function, genomics and functional genomics studies.The data presented suggest a combination of a constitutive resistance and an increased transcriptional response from P. purpurea when challenged with the pathogen. This knowledge opens new perspectives for the understanding of defence responses underlying pathogenic oomycete/plant interaction upon challenge with P. cinnamomi.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4042-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi

et al. 2017

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“…Nevertheless, data on long-term treatment with phosphite and its impact on the ecosystem are scarce, but it is known that in diseased habitats, phosphite treatment significantly reduced the loss of shrub cover, bare ground and sedge cover, but, it does not causes adverse/ negative impacts on species assemblages and structure [19,20]. Other options for pathogen control are the use of the fungicide fosetyl-aluminum [21], the treatment with copper salts to improve host resistance [22] and, the extract of Phlomis purpurea [23]. Furthermore, according to Dunstan et al, the most effective control is host removal, followed by fumigation and fungicide application in the soil [18].…”

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confidence: 99%

Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches

2020

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The Phytophthora genus is composed, mainly, of plant pathogens. This genus belongs to the Oomycete class, also known as "pseudo-fungi", within the Chromista Kingdom. Phytophthora spp. is highlighted due to the significant plant diseases that they cause, which represents some of the most economically and cultural losses, such as European chestnut ink disease, which is caused by P. cinnamomi. Currently, there have been four genome assemblies placed at the National Center for Biotechnology Information (NCBI), although the progress to understand and elucidate the pathogenic process of P. cinnamomi by its genome is progressing slowly. In this review paper, we aim to report and discuss the recent findings related to P. cinnamomi and its genomic information. Our research is based on paper databases that reported probable functions to P. cinnamomi proteins using sequence alignments, bioinformatics, and biotechnology approaches. Some of these proteins studied have functions that are proposed to be involved in the asexual sporulation and zoosporogenesis leading to the host colonization and consequently associated with pathogenicity. Some remarkable genes and proteins discussed here are related to oospore development, inhibition of sporangium formation and cleavage, inhibition of flagellar assembly, blockage of cyst germination and hyphal extension, and biofilm proteins. Lastly, we report some biotechnological approaches using biological control, studies with genome sequencing of P. cinnamomi resistant plants, and gene silencing through RNA interference (iRNA).

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“…In addition, a diversity of families, species, varieties and genetic diversity will increase the capacity of the system to survive and prosper in different climate conditions [110][111][112] thus increasing its resilience [113]. Diversity can include measures such as micorrization [114] to increase survival rates; composted manure [115,116] and the plant Phlomis purpurea to control pests [117]; diversification of fodder using edible shrubs adapted to drought [118] or increasing species richness to increase productivity [119]. c.…”

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confidence: 99%

Framework for Climate Change Adaptation of Agriculture and Forestry in Mediterranean Climate Regions

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Branquinho

et al. 2021

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Planning the adaptation of agriculture and forestry landscapes to climate change remains challenging due to the need for integrating substantial amounts of information. This information ranges from climate scenarios, geographical site information, socio-economic data and several possible adaptation measures. Thus, there is an urgent need to have a framework that is capable of organizing adaptation strategies and measures in the agriculture and forestry sectors in Mediterranean climatic regions. Additionally, this framework should provide a cause effect relation with climate vulnerability to adequately support the development of adaptation planning at municipal and local (farm) level. In this context, we propose to test and evaluate a framework for climate adaptation of the agriculture and forestry sectors, based on the local causal-effect relation between adaptation strategies and measures and the level of vulnerability reduction achieved for Mediterranean areas. The framework was developed based on the combination of the DPSIR (Driving forces, Pressures, State, Impacts, Responses) and Vulnerability frameworks and reviewed 162 practical adaptation measures, further organized into strategies, complemented by a set of efficacy indicators. The framework was tested with 70 stakeholders in six stakeholder workshops for the planning of two farms and one municipal climate adaptation study, that are now in actual implementation and monitoring. The framework is composed by a set of eight adaptation strategies in which adaptation measures are clustered and assessed using efficacy indicators. In the evaluation of the adaptation framework, 96% of stakeholders considered its content as good or very good and 89% considered the final outcomes as good or very good. Finally, the framework was also used to assess and compare the adaptation strategies and measures presented in the climate adaptation plans of the three case studies. On average, 52.2% of the adaptation measures selected by the three case studies are dedicated to Ecosystem Resilience, 30.9% to Adaptive Capacity, 9.1% to Microclimates, 7.4% to Protection, and 0.3% to Mitigation strategies. This framework was considered effective in supporting adaptation planning at farm and municipal levels and useful to assess and compare adaptation plans in the frame of vulnerability reduction. Future studies can further contribute to support adaptation planning in these sectors by using, developing and streamlining this framework to additional and different socio-ecological contexts.

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Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts

Cited by 14 publications

References 18 publications

De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi

De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi

Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches

Framework for Climate Change Adaptation of Agriculture and Forestry in Mediterranean Climate Regions

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