Anti-Photoaging Effects ofAngelica acutilobaRoot Ethanol Extract in Human Dermal Fibroblasts

Abstract

The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.

The Red Algae Compound 3-Bromo-4,5-dihydroxybenzaldehyde Protects Human Keratinocytes on Oxidative Stress-Related Molecules and Pathways Activated by UVB Irradiation

“…In addition, to assess the effect of BDB (Matrix Scientific, Columbia, SC, USA) against UVB-exposed cells, cells were treated with BDB at 10, 20, 30, 40, and 50 μM and exposed to UVB light at 30 mJ/cm 2 1 h later and incubated at 37 °C for one day. An MTT solution (Sigma-Aldrich Co., St. Louis, MO, USA) was added to each well, the formazan crystals were dissolved in DMSO, and cell viability was assessed by measuring the absorbance at 540 nm using a microplate reader [ 33 ]. The MTT experiment was repeated three times.…”

Preclinical safety assessment ofAngelica acutilobausing a 13-week repeated dose oral toxicity study in rats

“…Danggui, the root of the species Angelica belonging to the family Umbelliferae, has been named ‘female ginseng’ and has been most commonly used traditional herbal medicine for the treatment of gynecological disorders, such as menoxenia and anemia, due to its hemogenic, analgesic, and sedative activities [ 1 2 3 ]. According to geographical locations, three common species of Danggui are found in Asia: Korean Danggui ( Angelica gigas in Korea), Chinese Danggui ( A. sinensis in China), and Japanese Danggui ( A. acutiloba in Japan).…”

“…Further, that the root of Angelica acutiloba was reported to exert a protective effect against allergic diseases and inflammatory diseases through the inhibition of the release of histamine from mast cells and the production of pro-inflammatory cytokines [ 13 16 17 18 ]. Also, the AA could be a potent anti-wrinkle agent in the field of traditional medicine through the enhancement of collagen synthesis and suppression of matrix metalloproteinases [ 1 ].…”

Evaluation ofin vitroandin vivogenotoxicity ofAngelica acutilobain a standard battery of assays

“…Many species Angelica belonging to the family Umbelliferae has long been known as traditional herbal medicines that can be found throughout Asian countries, including Korea, China, and Japan. In particular, Danggui (root of Angelica) or ‘female ginseng’ is widely used as a typical therapeutic for treating gynecological disorders such as menoxenia, menstrual pain, anemia, and fatigue/low vitality [ 1 2 3 4 5 ]. Despite similarities in shapes, Danggui has different origin species and scientific names according to geographical locations: Angelica sinensis from China (Chinese Danggui), A. acutiloba (AA) from Japan (Japanese Danggui), and of A. gigas from Korea (Korean Danggui) [ 6 ].…”

“…In addition, it is reported that the AA inhibited the release of histamine from mast cells and the production of pro-inflammatory cytokines, which contributed to the protection against allergic diseases and inflammatory diseases [ 16 17 18 19 ]. Additional pharmacological effects of the AA include the enhancement of collagen synthesis and suppression of matrix metalloproteinases, resulting in its use as an anti-wrinkle natural agent [ 1 ].…”