Anti-photoaging effect of fermented rice bran extract on UV-induced normal skin fibroblasts

Abstract

Rice bran contains various polyphenolic compounds with anti-oxidative activities, and it has long been known to inhibit melanogenesis. Cofermentation of rice bran with Lactobacillus rhamnosus and Saccharomyces cerevisiae significantly reduced the melanin synthesis of the resulting extract to B16F1 melanoma cells. However, anti-aging and anti-inflammatory effects of these fermented rice bran extracts (FRBEs) were not investigated. The objective of this work was to investigate the ability of FRBE to protect fibroblast cultures against ultraviolet B (UV-B)-induced damage in vitro. To study the effect of FRBE on collagen synthesis, human fibroblasts were treated with 0.1, 0.5, or 1% of FRBE or 25 lM of As-2p over 3 days. The morphological change of fibroblasts, secretion of IL-1a, and pro-MMP-1 levels were evaluated. The rate of cell growth was reduced upon exposure of fibroblasts to UV-B radiation (30 mJ/cm 2 ) and did not recover after FRBE treatment. FRBE did not induce morphological changes and necrosis in a dose-dependent manner.Immunohistochemistry confirmed increased collagen synthesis in a dose-dependent manner after UV-irradiated fibroblasts were treated with FRBE. FRBE treatment reduced the UV-B-induced release of IL-1a to levels found in non-irradiated controls. In fibroblasts subjected only to UV-B irradiation, the level of pro-MMP-1 increased approximately fourfold compared to non-irradiated cells. However, treatment with FRBE decreased the level of pro-MMP-1 to a level similar to that of As-2p treated cells; this result was confirmed by western blot analysis. This work indicates that FRBE can increase the synthesis of type I collagen, decrease the expression of MMP-1, and inhibit the production of IL-1a in UV-B irradiated human fibroblasts.

Ameliorating Effects of Fermented Rice Bran Extract on Oxidative Stress Induced by High Glucose and Hydrogen Peroxide in 3T3-L1 Adipocytes

“…Previous studies have shown that rice bran fermented with Saccharomyces cerevisiae has anti-stress and anti-fatigue effects [16]. Furthermore, polysaccharide extracts of rice bran fermented with Lentinus edodes has anti-cancer properties, and water extracts of FRB which were stimulated by S. cerevisiae and Lactobacillus rhamnosus had an anti-photoaging effect [17,18]. FA and fractionized phenolic acid from rice bran exhibited hypoglycemic effects in a type 2 diabetic mice model [19].…”

Therapeutic properties of rice constituents and derivatives (Oryza sativa L.): A review update

“…A bran extract subjected to enzymatic fermentation has been evaluated on cultivated keratinocytes and in vitro-reconstructed skin, serving as a protective screen against UV-B radiation (Santa-Maria, Revilla et al 2010). Similarly, another bran fermented extract, obtained from Lactobacillus rhamnosus and Saccharomyces cerevisiae, has protected fibroblasts from in vitro exposure to UV-B rays (Seo, Jung et al 2010).…”

Rice bran extract affects differentiation of mesenchymal stem cells potency into osteogenic cells

“…Recent studies have reported that these extracts exert a number of biological effects on animal cells, including the following: enhancement of human natural killer cell activity (Ghoneum 1998); anti-photoaging effects in human fibroblasts (Seo et al 2010); and antiinflammatory effects in mice (Akihisa et al 2000). However, there is no report concerning the biological effects of RBE on MSCs.…”