Anti-oxidative stress effects of Herba leonuri on ischemic rat hearts

Sun, Jing; Huang, Shan; Zhu, Yi; Whiteman, Matthew; Wang, Min Jie; Tan, Benny Kwong Huat; Zhu, Yi Zhun

doi:10.1016/j.lfs.2004.11.024

Cited by 54 publications

(38 citation statements)

References 29 publications

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“…Our previous study showed that purified Herba Leonuri preserves antioxidant properties and does have a cardioprotective effect on ischemic myocardium through an antioxidant effect. 7 We have also demonstrated that the extract has neuroprotective effects on middle cerebral arteryoccluded rats through its antioxidant effect. 8 Leonurine (4-guanidino-n-butyl-syringate; Figure 1) is an alkaloid present in Herba Leonuri.…”

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confidence: 70%

Leonurine Protects Middle Cerebral Artery Occluded Rats Through Antioxidant Effect and Regulation of Mitochondrial Function

Loh

Jia

Tan

et al. 2010

Stroke

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Background and Purpose-Oxidative stress is known to be involved in ischemic stroke. Intense interest is drawn to the therapeutic potential of Chinese herbs on ischemic stroke because many of them contain antioxidant properties. Leonurine, 1 of the active compounds from purified Herba Leonuri, was studied to evaluate its possible therapeutic effects on ischemic stroke. Method-Middle cerebral artery occlusion was selected as our model of study. The animals were pretreated with Leonurine orally for 7 days and the surgery was done. One day after surgery, 2,3,5-triphenyltetrazolium chloride staining and neurological deficit score were carried out to evaluate the functional outcome of animals, whereas levels of superoxide dismutase, glutathione peroxidase, and malondialdehyde were analyzed for oxidative stress analysis. For mitochondrial studies, 3 hours after surgery, mitochondria were isolated for analysis of reactive oxygen species production, adenosine triphosphate biosynthesis, oxygen consumption, and respiratory control ratio value. Result-In in vivo experiments, Leonurine pretreatment reduced infarct volume, improved neurological deficit in stroke groups, increased activities of antioxidant enzymes superoxide dismutase and glutathione peroxidase, and decreased levels from the lipid peroxidation marker malondialdehyde. In terms of mitochondrial modulation, Leonurine inhibited mitochondrial reactive oxygen species production and adenosine triphosphate biosynthesis. Animal studies also demonstrated that the mitochondrial function and redox state were restored by Leonurine treatment. Conclusions-Leonurine has neuroprotective effects and carries a therapeutic potential of stroke prevention. (Stroke.

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confidence: 70%

Leonurine Protects Middle Cerebral Artery Occluded Rats Through Antioxidant Effect and Regulation of Mitochondrial Function

Loh

Jia

Tan

et al. 2010

Stroke

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124

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“…The external solution used to record I Ca,L contains (mM): tetraethylammonium chloride (TEA-Cl) 120, HEPES 10, MgCl 2 1, TTX 0.001, CsCl 10, CaCl 2 2 and glucose 10, pH 7.4(TEAOH) and that for recording I Na had the following composition (mM): NaCl 137, MgCl 2 to +50 mV (with 10 mV increments), then back to −40 mV. The amplitude of I K,tail was measured with a 1 s latency following the end of the depolarizing step.…”

Section: Methodsmentioning

confidence: 99%

“…Studies have shown that HL extracts ameliorated myocardial ischemia and improved heart functions. 1,2) Leonurine (Leo, 4-guanidino-n-butyl syringate, also named as ) is the special alkaloid for HL. Our group carried out a series of studies focusing on its cardioprotective effect.…”

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confidence: 99%

Effects of <i>Leonurine</i> on L-Type Calcium Channel in Rat Ventricular Myocytes

Hong

Wang

et al. 2012

Biological & Pharmaceutical Bulletin

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Leonurine (Leo) is a special alkaloid principle of Herba leonuri that has recently been suggested to improve cardiovascular functions. To date, there is no direct ionic evidence of Leo on regulating calcium channels in the heart. In the present study, we examined the effects of Leo on action potentials and membrane currents recorded from isolated rat ventricular myocytes with the whole-cell patch clamp technique. Leo 100 µM shortened the action potential duration in a dose-dependent manner. Leo up to 200 µM had no significant effect on the Na current (I Na ) and K current (I K ). However, Leo depressed the L-type Ca 2 current (I Ca,L ). In the presence of 20 and 100 µM Leo, the current density was decreased and the voltage at half maximal inactivation V 0.5 shift to more negative potential. The recovery time constant was also delayed. In addition, the transcription and protein expression levels of L-type calcium channel (Ca v1.2 ) in primary cultured neonatal myocytes from Sprague-Dawley rats were reduced by Leo treatment in a dose-dependent fashion as assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assays. We conclude that Leo inhibits L-type calcium channels in cardiomyocytes.

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“…Antioxidants have been of growing interest recently since they are supposed to reduce oxidative damage (Zhu et al 2004) that involved in many diseases such as ischaemic diseases (Ji et al 2003;Sun et al 2005), inflammation (Wang et al 2004), Alzheimer's diseases (Christen 2000), cancer, and diabetes (Anderson et al 2004;Stanner et al 2004). DPPH, which is a stable free radical and accepts an electron or hydrogen radical to become a stable diamagnetic molecule (Soares et al 1997), has been used widely to evaluate antioxidant activities because the model of scavenging DPPH radical can detect a large number of samples in a short period and is sensitive enough to the compounds at low concentrations (Russo et al 2005).…”

Section: Discussionmentioning

confidence: 99%

The in vitro anti-platelet, antioxidant and cellular immunity activity of Phellinus gilvus fractional extracts

Chang

Hwang

Rhee

et al. 2007

World J Microbiol Biotechnol

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The in vitro anti-platelet and antioxidant activities of various solvent extracts from Phellinus gilvus (PG), and the effects of hot water extract from PG (PGW) on murine cellular immunity were investigated. Chloroform extract (CE), methanol extract (ME) and butanol extract (BE) from PG could significantly inhibit platelet aggregation induced by thrombin. Ethyl acetate extract (EAE), BE, ME from PG had significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the control, and the EAE showed the highest effect with IC 50 values of 13.34 lg/ml, which is higher than that of ascorbic acid (40 lg/ml). In addition, EAE displayed the inhibition of xanthine oxidase (XO) activity with IC 50 value of 2.45 lg/ml. As to the cellular immunity activity, PGW could enhance both the lipopolysaccharide (LPS)-induced B lymphocyte proliferation and concanavalin A (Con A)-induced T lymphocyte proliferation in vitro. The phagocytosis of both peritoneal macrophages and RAW264.7 macrophage cells were also increased by the addition of PGW. Moreover, PGW was found to inhibit the nitric oxide (NO) production of RAW264.7 macrophages induced by LPS in a concentration-dependant manner.

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Anti-oxidative stress effects of Herba leonuri on ischemic rat hearts

Cited by 54 publications

References 29 publications

Leonurine Protects Middle Cerebral Artery Occluded Rats Through Antioxidant Effect and Regulation of Mitochondrial Function

Leonurine Protects Middle Cerebral Artery Occluded Rats Through Antioxidant Effect and Regulation of Mitochondrial Function

Effects of <i>Leonurine</i> on L-Type Calcium Channel in Rat Ventricular Myocytes

The in vitro anti-platelet, antioxidant and cellular immunity activity of Phellinus gilvus fractional extracts

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