Anti-Oxidative and Anti-Aging Activity of Extracts of Sprouts of Panax ginseng Fermented with a Lactic Acid Bacteria Mix of Lactobacillus spp. and Weissella spp.

Abstract

In this study, the amounts of functional ingredients such as γ-aminobutyric acid (GABA), ornithine, and saponin in fermented sprout ginseng (FSG) extracts were analyzed. The FSG was prepared using a lactic acid bacteria mix (Lactobacillus brevis OPK-3, Weissella koreensis OK1-6, and Weissella confusa JK27), and extracted with ethanol, followed by n-hexane, methylene chloride, ethyl acetate, and n-butanol fractionation. The sprouted ginseng fermented using mixed lactic acid bacteria showed increased GABA and ornithine contents. The results of measuring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities of the FSG extract and fractions, confirmed that the methylene chloride fraction showed the best antioxidant activity. The FSG methylene chloride fraction increased the activities of superoxide dismutase and catalase in Caenorhabditis elegans (C. elegans), in a concentration-dependent manner, and significantly reduced the amount of reactive oxygen species generated in the nematode. In addition, it was confirmed that the methylene chloride fraction increased resistance to oxidative stress by increasing the expression of green fluorescent protein (GFP) in the body of the SOD-3::GFP-fused transgenic strain CF1553 of C. elegans in a concentration-dependent manner. It was also confirmed that the FSG methylene chloride fraction significantly increased the lifespan of C. elegans. Furthermore, the accumulation of lipofuscin, which promotes aging, was reduced in a concentration-dependent manner. Taking these results together, it is believed that FSG can contribute to the prevention of diseases related to oxidation and aging.

Effect of Protaetia brevitarsis larval frass fertilizer on the growth of ginseng sprout (Panax ginseng) in commercial potting soil

“…새싹인삼은 인삼의 주요 성분인 사포닌, 진세노사이드, 폴리페놀 등을 함유하고 있으며, 항산화, 항염증, 항암, 항노 화, 항당뇨 등의 효능이 있다 (Chae et al, 2022;Mun et al, 2023). 새싹인삼은 온도, 습도, 광량, 영양분 등의 환경 요 인과 병충해 방제 기술이 연구되고 있지만 (Choi et al, 2022;Song et al, 2022Song et al, , 2023…”