Anti-oxidant Function and Inhibitory Effects of the Expression of MITF, TRP-1, TRP-2 and Tyrosinase of Sesamum indicum L. in B16F10 Melanoma Cells

Abstract

This study was performed to improve the antioxidant and skin-whitening activities of 70% ethanol extract from Sesamum indicum L. (SIL). The electron-donating ability of the SIL extract was 71.7% at a concentration of 1,000 μg/ml. The whitening effects that was measured by tyrosinase inhibition assay. As a result, SIL extract was shown 42% at 1,000 μg/ml concentration. The cell toxicity on B16F10 melanoma cells of SIL of 70% ethanol extract showed 84.3% at 1,000 μg/ml concentration. The microphthalmia-associated transcription factor (MITF), tyrosinase relate protein-1 (TRP-1), tyrosinase relate protein-2 (TRP-2) and Tyrosinase protein and mRNA expression inhibitory effect of SIL extract were measured by western blot and reverse transcription-polymerase chain reaction (PCR) at 50, 250, 500 μg/ml concentration. Consequently, the MITF, TRP-1, TRP-2, Tyrosinase protein expression inhibitory effect of SIL extract was decreased by 68.3%, 39.2%, 89.7%, 22.3%, respectively, at 500 μg/ml concentration. Moreover, MITF, TRP-1, TRP-2, Tyrosinase mRNA expression inhibitory effect by reverse-transcription-PCR of SIL extract was decreased by 81.8%, 66.5%, 84.2%, 68.1%, respectively, at 500 μg/ml concentration. Therefore, we excellently identified the antioxidant activities and whitening effect of SIL extract, and this finding suggested that SIL extract has great potential as a cosmetic ingredients.

Antioxidant, α-amylase and α-glucosidase inhibitory ability effects of sesame meal ethanol extract

“…참깨박 탈지 및 추출물 제조 참깨박의 탈지는 선행연구을 참고하여 200 g의 참깨박에 3배 가 량의 n-hexane을 가하여 24시간 침지하고 여과하는 방법으로 3 회 반복하여 유지성분을 제거하였다[13]. 유지성분을 제거한 잔 사에 70% 에탄올로 5배 추출을 하고 초음파 추출기(KUS-650, KBT, Sungnam, Korea) 25 o C, 300 W에서 20분씩 3회 반복하 여 추출하였다.…”