Anti-oxidant and anti-inflammatory effects of Fraxinus rhynchophylla on lipopolysaccharide (LPS)-induced murine Raw 264.7 cells

Hong, Gyeong-Eun; Park, Hyeonsoo; Kim, Jina; Nagappan, Arulkumar; Zhang, Jue; Kang, Suk-Yun; Won, Chung-Kil; Cho, Jae‐Hyeon; Kim, Eun‐Hee; Kim, Gon‐Sup

doi:10.12729/jbr.2012.13.4.331

Cited by 8 publications

(9 citation statements)

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“…Oxidative stress is generally defined as excess formation and/or insufficient removal of highly reactive molecules, such as ROS and reactive nitrogen species (Lim et al, 2010;Hong et al, 2012). Based on the results of previous studies, LPO, GSH depletion, DNA oxidation, increased intracellular Ca 2+ , and generation of excess ROS are potential mechanisms of 1,3-DCP-indcued toxicity (Katoh et al, 1998;Hammond and Fry, 1999;.…”

Section: Tablementioning

confidence: 99%

Time-course and molecular mechanism of hepatotoxicity induced by 1,3-dichloro-2-propanol in rats

Lee

et al. 2015

Environmental Toxicology and Pharmacology

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Section: Tablementioning

confidence: 99%

Time-course and molecular mechanism of hepatotoxicity induced by 1,3-dichloro-2-propanol in rats

Lee

et al. 2015

Environmental Toxicology and Pharmacology

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“…Lipopolysaccharide (LPS), a macrophage activator, is known to rapidly activate morphological changes in macrophages. It has been reported that anti-inflammatory agents can generally inhibit the LPS-stimulated cell morphological changes [ 22 , 23 ]. To evaluate the effects of GOS on the morphologies of LPS-stimulated RAW264.7 cells, the cells were monitored under a phase contrast microscope (×40).…”

Section: Resultsmentioning

confidence: 99%

“…It is worth noting that LPS, which is a well-known macrophage activator, is known to inhibit proliferation, induce apoptosis [ 50 ], and stimulate macrophage morphological changes [ 22 , 23 ]. LPS-induced apoptosis in macrophages results from two independent mechanisms: first and predominantly, this activity occurs through the autocrine secretion of TNF-α (early apoptotic events), and second, it occurs through the production of NO (late phase of apoptosis) [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

et al. 2015

Marine Drugs

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Alginate is a natural polysaccharide extracted from various species of marine brown algae. Alginate-derived guluronate oligosaccharide (GOS) obtained by enzymatic depolymerization has various pharmacological functions. Previous studies have demonstrated that GOS can trigger the production of inducible nitric oxide synthase (iNOS)/nitric oxide (NO), reactive oxygen species (ROS) and tumor necrosis factor (TNF)-α by macrophages and that it is involved in the nuclear factor (NF)-κB and mitogen-activated protein (MAP) kinase signaling pathways. To expand upon the current knowledge regarding the molecular mechanisms associated with the GOS-induced immune response in macrophages, comparative proteomic analysis was employed together with two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and Western blot verification. Proteins showing significant differences in expression in GOS-treated cells were categorized into multiple functional pathways, including the NF-κB signaling pathway and pathways involved in inflammation, antioxidant activity, glycolysis, cytoskeletal processes and translational elongation. Moreover, GOS-stimulated changes in the morphologies and actin cytoskeleton organization of RAW264.7 cells were also investigated as possible adaptations to GOS. This study is the first to reveal GOS as a promising agent that can modulate the proper balance between the pro- and anti-inflammatory immune responses, and it provides new insights into pharmaceutical applications of polysaccharides.

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“…Esculetin is a derivative of coumarin that is present in Chinese herbal drug 'Qinpi', used as folk medicines, such as Fraxinus rhynchophylla, Rehmanniae glutinosa and Artemisia capillaries. [20][21][22][23] Previously, the inhibitory effect of Esculetin on cell proliferation in human cell lines had been reported. 24,25 Esculetin reduced CCl4-induced hepatic apoptosis in rats probably against hepatocellular apoptosis with its free-radical scavenging ability.…”

Section: Discussionmentioning

confidence: 99%

Topical administration of Esculetin as a potential therapy for experimental dry eye syndrome

Jiang

Liu

2017

Eye

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PurposeIn this study, we investigated the therapeutic effects of topical Esculetin for dry eye rabbits through the ocular tests, inflammatory factor levels and specific phosphorylated protein expressions of ERK1/2 singnal pathway.Patients and methodsThirty-two healthy adult male New Zealand white rabbits were chosen for the study. DES models were established after removing of the main lacrimal gland, Harderian gland and nictitating membrane in the left eyes and randomly divided into group DES control, group CsA, group Esculetin and group Esculetin combined with CsA (C&E), meanwhile the right eyes served as group Normal control. Schirmer's I tests, fluorescein scores, goblet cell densities, inflammatory cytokines IL-1α,IL-1β,TNF-αlevels were observed by slit-lamp microscope, conjunctival impression cytology and ELISA essay at week 0, 1, 2, 4, 8. Phosphorylated-ERK1/2 expressions were detected in Western blot analysis at week 8.ResultsAfter induction of DES, aqueous tear production and goblet cell density were decreased, FL score was much higher in group DES control throughout the study (P<0.05). Both topical Esculetin and Esculetin combing CsA increased the SIT values (10±1 mm, 14±3 mm, P<0.05) and goblet cell densities (77±12/HP, 92±12/HP, P<0.05), decreased FL scores (7.48±0.33, 5.09±0.24, P<0.05) at week 8. Alternations of IL-1α,IL-1β,TNF-αlevels had similar trend. In Western blot analysis, downregulations of p-ERK1/2 were observed in therapy groups when compared with group DES control and the most decreasing was found in group C&E (P<0.05).ConclusionTopical Esculetin improved DES symptoms, downregulated the inflammatory cytokine expressions, suppressed the ERK1/2 pathway and enhanced the therapeutic effect of CsA.

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Anti-oxidant and anti-inflammatory effects of Fraxinus rhynchophylla on lipopolysaccharide (LPS)-induced murine Raw 264.7 cells

Cited by 8 publications

References 1 publication

Time-course and molecular mechanism of hepatotoxicity induced by 1,3-dichloro-2-propanol in rats

Time-course and molecular mechanism of hepatotoxicity induced by 1,3-dichloro-2-propanol in rats

Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

Topical administration of Esculetin as a potential therapy for experimental dry eye syndrome

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