Anti‐oxidant and anti‐fatigue properties of apple pomace polysaccharides by acid or alkali extraction

Abstract

The anti-fatigue effects and structure-activity relationship of apple polysaccharides (APPs), the active components in apple pomace, remain unknown. We examined the effects of different extraction methods on polysaccharide structures and anti-fatigue activities. Additionally, the structural and biochemical properties of APPs prepared under acidic (ACAPP) or alkaline (ALAPP) conditions, were characterised. ACAPP and ALAPP were acidic heteropolysaccharides primarily comprising arabinose, rhamnose, galactose, glucose, xylose and galacturonic acid. The molecular weights of ACAPP and ALAPP were 110 kDa and 89.6 kDa, respectively. ALAPP had more branched chains than ACAPP and showed stronger free radical-scavenging and anti-fatigue properties. The anti-fatigue properties of APPs correlated with their anti-oxidant activities and were exerted through eliminating free radicals and mediating an oxidative balance. The stronger anti-fatigue activity of ALAPP is probably related to its structural characteristics. Collectively, our study provides a fundamental basis for the development and use of APPs in medicine and functional foods.

Polysaccharides from steam‐processed Polygonatum cyrtonema Hua protect against d‐galactose‐induced oxidative damage in mice by activation of Nrf2/HO‐1 signaling

“…The stronger free radical scavenging abilities of PCPS may therefore be due to higher levels of uronic acid and reducing ends in comparison with PCPC. The monosaccharide type and/or content as well as the molecular weight are important factors for determining bioactivity 25,35 . Differences in monosaccharide composition and molecular weight may also be a factor affecting the high antioxidant activity of PCPS.…”

“…The monosaccharide type and/or content as well as the molecular weight are important factors for determining bioactivity. 25,35 Differences in monosaccharide composition and molecular weight may also be a factor affecting the high antioxidant activity of PCPS. It has been reported the triple-helix conformation of polysaccharides plays an important role in their biological activity.…”

Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats

“…Oxidative stress during strenuous exercise may lead not only to exercise-induced fatigue, but also to muscle damage [45]. During exercise, reactive oxygen species (ROS) will be generated, resulting in the oxidation of proteins, lipids or nucleic acids, which further aggravates oxidative stress [46].…”

Purification, Structural Characterization, and Antitumor Activity of a Polysaccharide from Perilla Seeds

“…After dissolving the crude polysaccharide in deionized water, free protein was precipitated using the Sevage method, and the supernatant was collected and freeze-dried. Decolorization was performed using the polyamide chromatography column method [42], and the eluate was collected, concentrated through evaporation under reduced pressure, and freeze-dried, yielding the Perilla seed polysaccharide (PFSP).…”