Anti-osteoporotic potential of Lactobacillus plantarum AR237 and AR495 in ovariectomized mice

Yu, Jing; Xia, Yongjun; Wang, Guangqiang; Xiong, Zhiqiang; Zhang, Hui; Lai, Phoency F.-H.; Song, Xin

doi:10.1016/j.jff.2021.104762

Cited by 7 publications

(10 citation statements)

References 50 publications

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“…Levels of bone alkaline phosphatase (BALP) and endotoxin in serum were detected by commercial kits (TW Reagent). The detection and calculation method of creatinine and deoxypyridinoline (DPD) and fluorescein isothiocyanate-dextran (FITC) were consistent with those of our previous study [33].…”

Section: Detection Of Biochemical Parameters Fluorescein Isothiocyana...supporting

confidence: 87%

“…The relative gene expression was calculated by 2 −∆CT method. The detailed information of cDNA amplification and 16SrDNA sequencing were consistent with those of our previous study [33].…”

Section: Real-time Quantitative Polymerase Chain Reaction Mus Primers...supporting

confidence: 85%

“…A total of 23 female C57BL/6 mice (8 weeks, specific-pathogen-free) were obtained from the JSJ Laboratory Animal Company (Shanghai, China) and were reared in the Laboratory Animal Center of Jiangnan University (Wuxi, China). The feeding conditions and surgical protocols were consistent with those described in our previous study [33]. Briefly, sixteen mice were subjected to the ovx operation, whereas seven mice were treated with a sham operation as controls.…”

Section: Animal Studymentioning

confidence: 99%

“…The biomechanical properties (bending stress, bending load, and Young's bending modulus) of femoral diaphysis were measured by a texture analyzer [34]. The test conditions of the micro-CT and biomechanics have been described in our previous study [33].…”

Section: Micro-computed Tomography and Biomechanical Detectionmentioning

confidence: 99%

“…Cells were digested with trypsin (WISENT, Nanjing, China), and the medium was removed. RNA isolation and cDNA synthesis have been described in our previous study [33].…”

Section: Rna Extraction From Small Intestine Tibia Fecal Samples and ...mentioning

confidence: 99%

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Anti-Osteoporotic Effect of Lactobacillus brevis AR281 in an Ovariectomized Mouse Model Mediated by Inhibition of Osteoclast Differentiation

Hang

Sun

et al. 2022

Biology

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Osteoporosis is a global disease characterized by weakened bone microarchitecture, leading to osteoporotic fractures. Estrogen replacement therapy is the traditional treatment for osteoporosis but carries with it an increased risk of cardiac events. In search of a safe and effective treatment, we used Lactobacillus brevis AR281, which has anti-inflammatory properties, to conduct a 7-week experiment, investigating its inhibitory effects on osteoporosis in an ovariectomized (ovx) mouse model. The results demonstrated that AR281 significantly improved bone microarchitecture and biomechanical strength in ovx mice by attenuating bone resorption. AR281 significantly decreased the critical osteoclast activator, the ratio of the receptor activator for nuclear factor kappa B (NF-κB) ligand (RANKL) to osteoprotegerin, and pro-inflammatory osteoclastogenic mediators, such as IL-1, IL-6, and IL-17, which can increase the RANKL expression. Moreover, AR281 modulated intestinal microbiota in ovx mice increased the abundance of Akkermansia, which is responsible for the improvement of gut epithelial barrier integrity. In an in vitro trial, AR281 suppressed the number of osteoclasts differentiated from the osteoclast precursor RAW264.7 cells caused by RANKL through the tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6)/NF-κB/nuclear factor of activated T cells c1 (NFATc1) pathway. Therefore, AR281 may be a natural alternative for combating osteoporosis.

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Section: Detection Of Biochemical Parameters Fluorescein Isothiocyana...supporting

confidence: 87%

Section: Real-time Quantitative Polymerase Chain Reaction Mus Primers...supporting

confidence: 85%

Section: Animal Studymentioning

confidence: 99%

Section: Micro-computed Tomography and Biomechanical Detectionmentioning

confidence: 99%

“…Cells were digested with trypsin (WISENT, Nanjing, China), and the medium was removed. RNA isolation and cDNA synthesis have been described in our previous study [33].…”

Section: Rna Extraction From Small Intestine Tibia Fecal Samples and ...mentioning

confidence: 99%

See 3 more Smart Citations

Anti-Osteoporotic Effect of Lactobacillus brevis AR281 in an Ovariectomized Mouse Model Mediated by Inhibition of Osteoclast Differentiation

Hang

Sun

et al. 2022

Biology

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Structural difference analysis of gut microbiota in different bone mass populations and relevant probiotic (lactobacilli) supplementation strategies

Pan,

Yin,

et al. 2024

Food Frontiers

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Disruption of the bone metabolic balance with advancing age leads to an escalating prevalence of bone‐related diseases, significantly compromising individuals’ quality of life. The gut microbiota actively participates in the regulation of bone metabolism, and perturbations in the gut microbiota can exacerbate bone diseases by compromising gut barrier integrity. Determining the microbial taxa involved in bone loss could offer valuable insights into the development of alternative therapies and nutritional interventions for disease management. Therefore, based on metagenomic and 16S ribosomal RNA data, this study analyzed the gut microbiota structure of 488 individuals with different bone masses (NC, normal; ON, osteopenia; OP, osteoporosis) to identify significant associations between the gut microbiota and bone loss. The results showed that at the genus and species levels, the microbiota diversity of the ON population increased, whereas that of the OP population decreased. Bacteroides were significantly enriched in the OP population, whereas the beneficial bacteria Bifidobacterium, Akkermansia, and lactobacilli decreased. Subsequent analyses revealed no significant variation in different bone populations in terms of Bifidobacterium levels, whereas lactobacilli exhibited diverse responses across distinct bone populations. The administration of lactobacilli effectively enhanced lumbar spine bone mineral density and modulated the gut microbiota structure in a population with unhealthy bone mass. This study contributes to the validation of the association between the gut microbiota and bone mass, enhances our understanding of the potential impact of probiotics (lactobacilli) on bone mass, and establishes a robust scientific basis for the application of probiotics in the regulation of bone mass.

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Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

et al. 2023

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Anti-osteoporotic potential of Lactobacillus plantarum AR237 and AR495 in ovariectomized mice

Cited by 7 publications

References 50 publications

Anti-Osteoporotic Effect of Lactobacillus brevis AR281 in an Ovariectomized Mouse Model Mediated by Inhibition of Osteoclast Differentiation

Anti-Osteoporotic Effect of Lactobacillus brevis AR281 in an Ovariectomized Mouse Model Mediated by Inhibition of Osteoclast Differentiation

Structural difference analysis of gut microbiota in different bone mass populations and relevant probiotic (lactobacilli) supplementation strategies

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

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