Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice

Park, Eunkuk; Kim, Jeonghyun; Yeo, Subin; Lim, Eunguk; Choi, Chun Whan; Choi, Sangho; Li, Wan Yi; Lee, Jiwon; Park, Jin-Hyok; Huh, Dam; Jeong, Seon‐Yong

doi:10.3390/nu11112716

Cited by 14 publications

(32 citation statements)

References 32 publications

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“…Isolated cells were incubated with RANKL and M-CSF for osteoclast differentiation and co-treated with single or three different ratios of CO and AJ mixtures (9:1, 8:2, and 7:3) for 6 days. As a result, both CO and AJ (10 and 50 μg/mL) extract in our study did not affect cellular proliferation ( Supplementary Figure S3 ), but inhibited TRAP activity ( Supplementary Figure S4 ), as previously described in the literature [ 18 , 32 ]. In addition, mixtures of CO and AJ did not show toxic effect to osteoclasts during the differentiation period ( Figure 3 B).…”

Section: Resultssupporting

confidence: 87%

Anti-Osteoporotic Effects of the Herbal Mixture of Cornus officinalis and Achyranthes japonica In Vitro and In Vivo

Park

Lee

Kim

et al. 2020

Plants

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Osteoporosis is a porous bone disease caused by bone density loss, which increases the risk of fractures. Cornus officinalis (CO) and Achyranthes japonica (AJ) have been used as traditional herbal medicine for various disorders in East Asia. Although the anti-osteoporotic effects of single extract of CO and AJ have already been reported, the synergistic effect of a combined mixture has not been studied. In this study, we investigated the effects of a CO and AJ herbal mixture on osteoporosis in in vitro and in vivo models. The results demonstrate that treatment with the CO and AJ mixture significantly promoted osteoblast differentiation of MC3T3-E1 mouse preosteoblasts through the upregulation of osteoblastic differentiation-associated genes such as alkaline phosphatase (Alpl), runt-related transcription factor 2 (Runx2), and bone gamma-carboxyglutamic acid-containing protein (Bglap), while the mixture significantly inhibited differentiation of osteoclasts isolated from primary-cultured mouse monocytes. In addition, oral administration of CO and AJ mixture significantly prevented bone mineral density loss and trabecular bone structures in an ovariectomy-induced osteoporotic mouse model. These results suggest that the combination treatment of CO and AJ mixture might be a beneficial therapy for osteoporosis.

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Section: Resultssupporting

confidence: 87%

Anti-Osteoporotic Effects of the Herbal Mixture of Cornus officinalis and Achyranthes japonica In Vitro and In Vivo

Park

Lee

Kim

et al. 2020

Plants

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“…Runx2 is an essential transcription factor for proper osteoblast differentiation; its expression is upregulated in immature osteoblast cells. Additionally, osteocalcin is secreted from osteoblasts and plays an important role in the regulation of bone metabolism [ 30 ]. Park et al reported that osteoclastogenesis is related to the high expression of these bone metabolic markers [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, osteocalcin is secreted from osteoblasts and plays an important role in the regulation of bone metabolism [ 30 ]. Park et al reported that osteoclastogenesis is related to the high expression of these bone metabolic markers [ 30 ]. Our results showed that CC treatment increased ALP gene expression.…”

Section: Discussionmentioning

confidence: 99%

Preventive Effects of Chrysanthemum coronarium L. Extract on Bone Metabolism In Vitro and In Vivo

Kim

Lee

Hur

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Osteoporosis is characterized by decreased bone mass and bone microarchitectural failure, leading to an enhanced risk of bone fractures. Chrysanthemum coronarium L. (CC) is a natural plant with powerful antioxidant activity. This study investigated the antiosteoporotic effects of CC extracts in in vitro cell cultures and in vivo bone loss animal models. CC stimulated osteoblast differentiation and mineralized bone formation by osteoblasts by increasing the expression of bone formation markers (alkaline phosphatase, osteoprotegerin, and osteoprotegerin/receptor activator nuclear factor-κB ligand ratio) in the murine preosteoblastic cell line MC3T3-E1. Additionally, CC was found to inhibit osteoclast differentiation by downregulating bone resorption markers (tartrate-resistant acid phosphatase, cathepsin K, dendritic cell-specific transmembrane protein, and calcitonin receptor) in the murine macrophage-like cell line RAW264.7. CC prevented ovariectomy-induced bone loss, preserved trabecular microarchitecture, and improved serum bone turnover markers in an osteoporotic mouse model. These findings suggest that CC extract may be considered as a natural therapeutic or preventive agent for osteoporotic bone loss.

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“…The maximum load in Newton (N), Young's modulus (MPa), maximum stress (MPa), stiffness (N/mm), and energy absorption (N•mm) were expressed from the load-deflection curve, and all parameters were calculated according to previously stated methods. [6,10] 2.11 | Pro-inflammatory cytokines…”

Section: Biomechanical Estimationmentioning

confidence: 99%

“…[4,5] With the rise in the aging population, the incidence of osteoporotic fractures and osteoporosis is growing rapidly. [6] The deficiency of hormones impairs cancellous metaphyseal bone and decrease bone mineral density (BMD) in animals and humans. However, estrogen deficiency has been considered a vital cause of the vulnerability of this population to osteoporosis in postmenopausal women.…”

Section: Introductionmentioning

confidence: 99%

Antiosteoporosis effect of geraniin on ovariectomy‐induced osteoporosis in experimental rats

Wang

Cui

et al. 2021

J Biochem & Molecular Tox

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Osteoporosis is a skeletal condition that is characterized by decreasing bone density and deteriorating bone mass. The plant-based phytoconstituent such as geraniin possesses better therapeutic potentials in biomedical field. In the current experimental study, we planned to scrutinize the therapeutic potential of geraniin against ovariectomy (OVX)-induced osteoporosis in rats and find the possible mechanism.Healthy Sprague Dawley rats were randomized into six groups and subjected to geraniin and alendronate (ALN) treatment for 10 weeks. Body weight, uterus, femur weight, bone biochemical, bone turnover markers, inflammatory cytokine, calcium, phosphorus, vitamin D (Vit D), urine, hormones, and antioxidant level were estimated. Geraniin significantly (p < .001) reduced the level of bone turnover markers including beta-CrossLaps (β-CTx), ALN, osteocalcin (OC), alkaline phosphatase (ALP), and bone Gla protein (BGP); reduced the biomechanical parameters including maximum load, energy, stiffness, maximum stress, and Young's modulus; reduced the level of calcium (Ca) and phosphorus (P); and increased the level of vitamin D (Vit D) as compared with OVX-induced osteoporosis rats. Geraniin increased the level of bone structure parameters, namely bone mineral density, bone mineral content, tissue mineral density, bone volume fraction, and trabecular number; increased the level of osteoprotegerin (OPG) and OPG/RANKL; and reduced the level of receptor activator of nuclear factor kappa-Β ligand (RANKL). Geraniin significantly (p < .001) increased the level of glutathione (GSH) and reduced the level of malonaldehyde (MDA) in the liver, intestine, and bone of OVX-induced osteoporosis rats. Geraniin significantly (p < .001) decreased the level of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) pro-inflammatory cytokines.We also argue that geraniin could be an excellent candidate to treat and control bone-related disease or disorders.

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Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice

Cited by 14 publications

References 32 publications

Anti-Osteoporotic Effects of the Herbal Mixture of Cornus officinalis and Achyranthes japonica In Vitro and In Vivo

Anti-Osteoporotic Effects of the Herbal Mixture of Cornus officinalis and Achyranthes japonica In Vitro and In Vivo

Preventive Effects of Chrysanthemum coronarium L. Extract on Bone Metabolism In Vitro and In Vivo

Antiosteoporosis effect of geraniin on ovariectomy‐induced osteoporosis in experimental rats

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