Anti-obesity potential of heat-killed Lactiplantibacillus plantarum K8 in 3T3-L1 cells and high-fat diet mice

Jang, Kyoung Ok; Choi, Jung Seo; Choi, Kyeong Hun; Kim, Seongjae; Kim, Hangeun; Chung, Dae Kyun

doi:10.1016/j.heliyon.2023.e12926

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…3T3-L1 cells are a cornerstone for obesity research because of their ability to differentiate into adipocytes. Thus, they are an invaluable model for understanding the intricacies of adipogenesis and its role in obesity and diabetes [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Shrestha,

Kim,

Shrestha

et al. 2024

J Anim Sci Technol

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Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα , was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT–PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ , FABP4 , adiponectin , and FAS , at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms.

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Section: Discussionmentioning

confidence: 99%

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Shrestha,

Kim,

Shrestha

et al. 2024

J Anim Sci Technol

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“…The metabolic health-beneficial effects of viable probiotic cell supplementation are likely associated with increased gastrointestinal colonization of the probiotic bacteria, promotion of the commensal population and inhibition of outgrowth of the pathobionts [ 1 ]. However, animal studies have shown that inactivated probiotic bacteria also hold the potential to mitigate experimental metabolic insults [ 24 ]. Indeed, clinical studies investigating inactivated L. plantarum are critically lacking, but a randomized, controlled trial demonstrated that daily supplementation of heat-inactivated L. plantarum L-137 reduced inflammation and improved lipid metabolism in 100 Japanese subjects [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism

et al. 2023

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Centering around the concept that metabolites from the gut commensals can exert metabolic health benefits along the gut–liver axis, we tested whether the cell-free global metabolome of probiotic bacteria can exert hepatoprotective benefits against H2O2-induced oxidative stress. Cell-free global metabolites of Lactobacillus plantarum (LPM) were isolated and untargeted metabolomics was performed. The free radical scavenging potentials of LPM were measured. The cytoprotective effects of LPM were tested on HepG2 cells. A total of 66 diverse metabolites were identified in LPM, among which saturated fatty acids, amino acids and dicarboxylic acids were highly enriched. LPM attenuated cell damage, lipid peroxidation and the levels of intracellular cytoprotective enzymes in H2O2-treated cells. LPM also attenuated H2O2-induced increased expressions of TNF-α and IL-6. However, the cytoprotective effects of LPM were diminished in cells that were pretreated with a pharmacological inhibitor of Nrf2. Our data collectively indicate that LPM can significantly attenuate oxidative damage to HepG2 cells. However, the cytoprotective effects of LPM likely depend on an Nrf2-dependent mechanism.

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“…The JAK2/STAT3 pathway may be inhibited by SOCS-1 which would then suppress the expression of genes related to the development of obesity. In contrast to the in vitro study, both live and heat-treated cells showed weight reduction in the animal model [ 133 ].…”

Section: Postbiotics and Anti-obesity Mechanismsmentioning

confidence: 94%

Postbiotics against Obesity: Perception and Overview Based on Pre-Clinical and Clinical Studies

Park

Sharma

Lee

2023

IJMS

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Overweight and obesity are significant global public health concerns that are increasing in prevalence at an alarming rate. Numerous studies have demonstrated the benefits of probiotics against obesity. Postbiotics are the next generation of probiotics that include bacteria-free extracts and nonviable microorganisms that may be advantageous to the host and are being increasingly preferred over regular probiotics. However, the impact of postbiotics on obesity has not been thoroughly investigated. Therefore, the goal of this review is to gather in-depth data on the ability of postbiotics to combat obesity. Postbiotics have been reported to have significant potential in alleviating obesity. This review comprehensively discusses the anti-obesity effects of postbiotics in cellular, animal, and clinical studies. Postbiotics exert anti-obesity effects via multiple mechanisms, with the major mechanisms including increased energy expenditure, reduced adipogenesis and adipocyte differentiation, suppression of food intake, inhibition of lipid absorption, regulation of lipid metabolism, and regulation of gut dysbiosis. Future research should include further in-depth studies on strain identification, scale-up of postbiotics, identification of underlying mechanisms, and well-defined clinical studies. Postbiotics could be a promising dietary intervention for the prevention and management of obesity.

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Anti-obesity potential of heat-killed Lactiplantibacillus plantarum K8 in 3T3-L1 cells and high-fat diet mice

Cited by 8 publications

References 35 publications

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism

Postbiotics against Obesity: Perception and Overview Based on Pre-Clinical and Clinical Studies

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