Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract

Jadeja, Ravirajsinh N.; Thounaojam, Menaka C.; Ramani, Umed; Devkar, Ranjitsinh; Ramachandran, A. V.

doi:10.1016/j.jep.2011.03.020

Cited by 28 publications

(20 citation statements)

References 26 publications

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“…Subsequently, treatment with ST could significantly ameliorate leptin resistance. Furthermore, accompanied by the reduced circulating leptin levels, the lowered serum TG, TC, LDL-C and FFA, and increased HDL-C levels by ST were consistent with previous studies in different animal models (Memon et al, 1994;Ahmed et al,1998;Rao et al, 1999;Kuo et al, 2009;Jadeja et al, 2011). However, ST has no dosedependent effect on these.…”

Section: Discussionsupporting

confidence: 90%

Sweet tea leaves extract improves leptin resistance in diet-induced obese rats

Zhou

Huang

Liu

et al. 2013

Journal of Ethnopharmacology

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Section: Discussionsupporting

confidence: 90%

Sweet tea leaves extract improves leptin resistance in diet-induced obese rats

Zhou

Huang

Liu

et al. 2013

Journal of Ethnopharmacology

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“…[22] Similarly, HFD-fed obese mice fed with aqueous extract of leaf of Clerodendron glandulosum (CG) recorded significant increment in size and mass of abdominal, renal, and epididymal fat pads, whereas this increment was prevented in CG-supplemented obese mice. [23]…”

Section: Resultsmentioning

confidence: 99%

Potential lipid-lowering effects ofEleusine indica(L) Gaertn. Extract on high-fat-diet-induced hyperlipidemic rats

2017

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Background:To date, anti-obesity agents based on natural products are tested for their potential using lipase inhibition assay through the interference of hydrolysis of fat by lipase resulting in reduced fat absorption without altering the central mechanisms. Previous screening study had indicated strong anti-obesity potential in Eleusine indica (E. indica), but to date, no pharmacologic studies have been reported so far.Objective:This study was performed to investigate the lipid-lowering effects of E. indica using both in vitro and in vivo models.Methods:The crude methanolic extract of E. indica was fractionated using hexane (H-Ei), dichloromethane (DCM-Ei), ethyl acetate (EA-Ei), butanol (B-Ei), and water (W-Ei). All the extracts were tested for antilipase activity using porcine pancreatic lipase. Because H-Ei showed the highest inhibition, it was further subjected to chemical profiling using high-performance liquid chromatography. Subsequently, oral toxicity analysis of H-Ei was performed [Organization for Economic Cooperation and Development guidelines using fixed dose procedure (No. 420)]; efficacy analysis was performed using high-fat diet (HFD)-induced hyperlipidemic female Sprague–Dawley rats.Results:According to the toxicity and efficacy analyses, H-Ei did not demonstrate any noticeable biochemical toxicity or physiologic abnormalities and did not cause any tissue damage as per histologic analysis. Furthermore, H-Ei significantly reduced body weight and improved serum profile and did not show hepatotoxicity and nephrotoxicity based on the serum profile. Moreover, H-Ei alleviated HFD-induced hepatosteatosis and ameliorated induced adiposity in both visceral and subcutaneous adipose tissue.Conclusion:Our results demonstrate that H-Ei effectively improved hyperlipidemia. Further studies to explore its possibility as an alternative pharmacologic agent to treat obesity are warranted.SUMMARY Hexane extract of Eleusine indica (H-Ei) showed strong potential in the inhibition of porcine pancreatic lipase (27.01 ± 5.68%).The acute oral toxicity of E. indica hexane extract on animal model falls into Globally Harmonized System Category 5 (low hazard), since mortality, clinical toxicity symptoms, gross pathologic, or histopathologic damage was not observed.The hexane extract of E. indica had significantly reduced the body weight and improved serum lipid profile, with reduction in serum triglycerides, total cholesterol, low-density lipoprotein, and elevation in high-density lipoprotein when comparing against the high-fat diet control group.Microscopic evaluation on histologic slides of liver and adipose tissues suggested that E. indica hexane extract had greatly improved liver steatosis and adipose tissue hypertrophy in high-fat diet control group. Abbreviation used: ALT: Alanine transaminase; AST: Aspartate transaminase; B-Ei: Butanol extract of E. indica; DCM-Ei: Dichloromethane extract of E. indica; EA-Ei: Ethyl acetate extract of E. indica; GHS: Globally Harmonized System; HDL: High-density lipoprotein; H-Ei: Hexa...

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“…Later (at day 0), culture media was replaced with DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine (Sigma Aldrich, USA), 0.25 μM dexamethosone (Sigma Aldrich, USA), and 10 μg/mL insulin(Sigma Aldrich, USA) and cells were maintained for 4 days. At the end of 4 days, culture media was replaced with maturation media containing complete DMEM with 10 μg/mL insulin and cells were maintained for another 8 days, subsequently media was replaced every 48 hr till the end of the experiment [20]. …”

Section: Methodsmentioning

confidence: 99%

Sida rhomboidea. Roxb Leaf Extract Down-Regulates Expression of PPARγ2 and Leptin Genes in High Fat Diet Fed C57BL/6J Mice and Retards in Vitro 3T3L1 Pre-Adipocyte Differentiation

Thounaojam

Jadeja

Ramani

et al. 2011

IJMS

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Sida rhomboidea. Roxb leaf extract (SRLE) is being used by the populace of North-East India to alleviate symptoms of diabetes and obesity. We have previously reported its hypolipidemic and anti-diabetic properties. In this study, we report the effect of SRLE on (i) in vivo modulation of genes controlling high fat diet (HFD) induced obesity and (ii) in vitro 3T3L1 pre-adipocyte differentiation and leptin release. Supplementation with SRLE significantly prevented HFD induced increment in bodyweight, plasma lipids and leptin, visceral adiposity and adipocyte hypertrophy. Also, SRLE supplementation reduced food intake, down regulated PPARγ2, SREBP1c, FAS and LEP expressions and up-regulated CPT-1 in epididymal adipose tissue compared to obese mice. In vitro adipogenesis of 3T3L1 pre-adipocytes was significantly retarded in the presence of SRLE extract. Also decreased triglyceride accumulation, leptin release and glyceraldehyde-3-Phosphate dehydrogenase activity along with higher glycerol release without significant alteration of viability of 3T3L1 pre-adipocytes, was recorded. Our findings suggest that prevention of HFD induced visceral adiposity is primarily by down regulation of PPARγ2 and leptin gene expression coupled with attenuation of food intake in C57BL/6J mice. SRLE induced prevention of pre-adipocytes differentiation, and leptin release further substantiated these findings and scientifically validates the potential application of SRLE as a therapeutic agent against obesity.

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Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract

Cited by 28 publications

References 26 publications

Sweet tea leaves extract improves leptin resistance in diet-induced obese rats

Sweet tea leaves extract improves leptin resistance in diet-induced obese rats

Potential lipid-lowering effects ofEleusine indica(L) Gaertn. Extract on high-fat-diet-induced hyperlipidemic rats

Sida rhomboidea. Roxb Leaf Extract Down-Regulates Expression of PPARγ2 and Leptin Genes in High Fat Diet Fed C57BL/6J Mice and Retards in Vitro 3T3L1 Pre-Adipocyte Differentiation

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