Anti-obesity effects of yellow catfish protein hydrolysate on mice fed a 45% kcal high-fat diet

Abstract

Obesity contributes to the etiologies of a variety of comorbid conditions, such as type 2 diabetes, hypertension and cardiovascular disease. In the present study, the anti-obesity effects of yellow catfish protein hydrolysate (YPh) were observed in mice fed a 45% kcal high-fat diet (HFD) compared with those of mice treated with simvastatin. The HFD-fed control mice exhibited noticeable increase in body weight, and whole-body and abdominal fat densities, periovarian and abdominal wall-deposited fat pad weight, as well as in the levels of triglycerides (TG), blood total cholesterol (TC), low-density lipoprotein, alanine aminotransferase, aspartate aminotransferase, creatinine, blood urea nitrogen, and in the fecal TG and TC contents. However, they exhibited a decrease in serum high-density lipoprotein levels. In addition, an increase was detected in periovarian and dorsal abdominally deposited fat pad thickness, adipocyte hypertrophy, the number of steatohepatitis regions, hepatocyte hypertrophy and lipid droplet deposition-related renal tubular vacuolation degenerative lesions, along with increased hepatic lipid peroxidation and a deteriorated endogenous antioxidant defense system (glutathione, catalase and superoxide dismutase). However, all the above-mentioned obesity-related complications were dose-dependently and significantly inhibited after 84 days of thye consecutive oral administration of 125, 250 and 500 mg/kg YPh. In addition, YPh dose-dependently depleted the liver endogenous antioxidant defense system and inhibited hepatic lipid peroxidation. Overall, the effects of 250 mg/kg YPh on HFD-induced obesity and related complications were similar or more potent than those of 10 mg/kg simvastatin. These results indicate that YPh is a promising new potent medicinal ingredient for possible use in the treatment of obesity and related complications.

Antihyperlipidemic and antioxidant effects of feather protein hydrolysate in high‐fat diet‐fed mice

“…Our results are in agreement with Kim et al. that showed that protein hydrolysate reduces the oxidative stress in vivo and increase the antioxidant activities.…”

“…This normalization is seen as an adaptation process, to face the oxidative stress by preventing the free radical production, so inhibiting the atherosclerosis development. Our results are in agreement with Kim et al [11] that showed that protein hydrolysate reduces the oxidative stress in vivo and increase the antioxidant activities.…”

“…Indeed, the antioxidant activities of protein hydrolysate and their abilities to inhibit cholesterol micellar solubility and interact with bile acids are demonstrated in vitro studies . Further, in vivo studies showed that the consumption of some protein hydrolysates by hyperlipidemic rats leds to a reduction in serum TC, TG, and LDL‐C and an increase in high‐density lipoprotein cholesterol (HDL‐C) and improves defense mechanisms .…”

Anti-obesity effects of Spirulina platensis protein hydrolysate by modulating brain-liver axis in high-fat diet fed mice

“…Many nutritional intervention studies have showed that food-derived proteins or hydrolysates can reduce body weight and fat mass, and lower plasma cholesterol and triglycerides. Such as, proteins of white lupin seed were reported to reduce cholesterolemia in rats [2]; germinated soy protein hydrolysates had inhibitory effect on lipid storage and stimulating activity on lipolysis in 3T3-L1 cells [3]; yeast hydrolysate suppressed body fat accumulation by attenuating fatty acid synthesis in high-fat-diet-induced obese mice [4]; dietary fish protein hydrolysates were found to influence serum lipids and postprandial glucose regulation in obese Zucker fa/fa rats [5]; enzymatic hydrolysate from velvet antler significantly reduced the body weight gain, lowered the serum glucose and triglyceride levels in high-fat diet-fed mice [6]; yellow catfish protein hydrolysate displayed anti-obesity effects in high-fat diet fed mice, compared with those of mice treated with simvastatin [7]. The potential anti-obesity mechanisms include appetite regulation, lipid digestion/absorption intervention, fatty acid oxidation, regulation of lipogenesis and lipolysis [1].…”

High Throughput Identification of Antihypertensive Peptides from Fish Proteome Datasets

“…Among these, fish hydrolysates have aroused enormous interests for their inexhaustible feature and high nutritional value [ 19 , 20 ]. Bioactive peptides with antimicrobial, antioxidant, anti-obesity, antihypertensive and cryoprotective effects from fish proteins in muscle, skin and even waste products have been reported [ 19 , 21 , 22 , 23 ]. Characterization of AHTPs in fish protein hydrolysates have been conducted in various species, such as salmon, tuna, grass carp and dace [ 24 , 25 , 26 , 27 ].…”