Anti-Obesity Effects of Ecklonia cava Extract in High-Fat Diet-Induced Obese Rats

Abstract

Obesity is becoming a global epidemic as a result of high-calorie food intake and unhealthy lifestyles. Different marine plants, especially brown algae (Ecklonia cava), are traditionally used to treat different health-related issues. The study was carried out to investigate the anti-obesity properties of E. cava 70% ethanol extract. To evaluate the anti-obesity effect of E. cava, both in vitro and in vivo tests were performed. E. cava suppresses pre-adipocyte 3T3-L1 differentiation in a dose-dependent manner. In HFD-induced obese rats’ models, administration of E. cava 125, 250, and 500 mg/kg significantly decreases total body weight and organs, especially liver weight, in all treatment groups. Adipose tissue weight, including subcutaneous, epididymal, peritoneal, and mesenteric adipose tissue, was markedly reduced in E. cava-treated HFD rats in dose-dependent manners. In addition, liver-related biomarkers AST, ALP, ALT, and GGT were evaluated; the lower level of liver-related biomarkers indicates no liver injury or fatty liver issue in E. cava HFD treatment groups. In addition, E. cava treatment has significant effects on the expression of adipogenic and lipogenic (PPAR-γ, FAS, LPL, and SREBP-1c) genes. Altogether, these results show the anti-obesity effect of E. cava. We concluded that E. cava could be a potential candidate for the prevention of obesity-induced by a high-fat diet.

Anti-atherogenic and cardio-protective properties of sweet melon (Cucumis melo. L. Inodorus) seed extract on high fat diet induced obesity in male wistar rats

“…Assessment of food and energy intake was carried out according to previous method [ 22 ] with modifications, The animal’s body weight and food intake was measured once a week during the study. The diet intake in each cage was calculated as: …”

“…But the most outstanding one that provides an accurate picture of human obesity is the diet-induced obesity model, which is usually carried out with a high-fat diet [ 21 ]. Previous reports also indicated that the use of rats is more appropriate for monitoring body weight change which is a major indicator of obesity [ 22 ]. Evidence from previous studies showed that treatment of high-fat- diet-fed rats with extracts of Gnidia glauca [ 23 ], Morinda citrifolia [ 24 ] , Anthocleista vogelii [ 25 ] and Silybum marianum [ 26 ] displayed strong anti-obesity activities by reducing organo-somatic and atherogenic indices.…”

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

“…These results indicate that onion and apple supplementation could be helpful in the prevention/treatment of hepatic steatosis in obese Zucker rats. In addition, different studies have reported that the intake of vegetable-enriched diets was associated with lowering effects in liver enzymes in rodent models [ 49 , 50 , 51 ].…”

Ethanolic extract of root from Arctium lappa L ameliorates obesity and hepatic steatosis in rats by regulating the AMPK / ACC / CPT ‐1 pathway

“…Rat diets were provided by XIETONG.ORGANISM Co., Ltd. Some studies found that the ethanol extract of plenty of plants has the function of treating hepatic steatosis (Abbas, Boby, Lee, Hong, & Park, 2022;Quan et al, 2022), but there is a lack of research on the treatment of obesity and liver steatosis with ethanol extract of Arctium lappa L root. Samples of 5 and 100 mg/kg•bw/day of ethanol extract of Arctium lappa L were used as the low monitoring dose and high monitoring dose respectively for the experiment.…”