Anti-obesity effects of Actinidia polygama extract in mice with high-fat diet-induced obesity

Sung, Yoon‐Young; Yoon, Taesook; Yang, Won‐Kyung; Moon, Byeong-Cheol; Kim, Ho Kyoung

doi:10.3892/mmr.2012.1239

Cited by 16 publications

(13 citation statements)

References 21 publications

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“…However, adipocyte accumulation in adipose tissue may cause obesity and related complications. Recent studies demonstrated that long-term HFD increases in serum TG, FFA, glucose, TC, HDL, and LDL decrease cholesterol levels, leading to an imbalance in serum lipid composition (24,34,35). This imbalance is considered as the main risk factor for several diseases.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice

Jung¹,

Kim²,

Min³

et al. 2019

In Vivo

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Background/Aim: The rapid increase in the number of people who are overweight or obese, which increases the risk of diseases and health problems, is becoming an important issue. Herein, we investigated whether olive leaf extract (OLE) has potent anti-obesity effects in high-fat induced mouse models. Materials and Methods: C57BL/6 mice were randomized into normal control, high-fat diet (HFD), HFD with OLE, and HFD with garcinia groups and administered experimental diets for 12 weeks. Body weight and food intake were measured once per week and obesity-related biomarkers were evaluated in the serum and adipose tissue. Results: OLE significantly suppressed weight gain, food efficiency ratio, visceral fat accumulation, and serum lipid composition in HFD-induced mice. Furthermore, the expression of adipogenesis-and thermogenesis-related molecules was decreased in the OLE-treated group. Conclusion: OLE prevents obesity development by regulating the expression of molecules involved in adipogenesis and thermogenesis.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice

Jung¹,

Kim²,

Min³

et al. 2019

In Vivo

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“…At the cellular level, obesity is characterized by an increase in the size (hypertrophy) and number (hyperplasia) of adipoctyes. Hence, a complete understanding of the mechanisms regulating adipogenesis is essential and of utmost priority ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/β-catenin signaling

Chen

Wang

et al. 2015

International Journal of Molecular Medicine

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MicroRNAs (miRNAs or miRs) play an important regulatory role during adipogenesis, and have been studied extensively in this regard. Specifically, the switch between the differentiation of mesenchymal stem cells (MSCs) towards adipogenic vs. osteogenic lineages is regulated by miR-204 which controls the expression of Runx2. However, the association between miR-204-5p and the Wnt/β-catenin signaling pathway during adipogenesis has not yet been clarified. In the present study, we demonstrate that miR-204-5p regulates the in vitro adipogenesis of human adipose-derived mesenchymal stem cells (hADSCs). The level of miR-204-5p was shown to be gradually upregulated during adipocytic differentiation, together with the mRNA expression of the critical adipogenic transcription factors, cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT) enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ), and the mature adipogenic marker, fatty acid binding protein 4 (FABP4). We further demonstrate that while the overexpression of miR-204-5p promotes adipogenesis, its knockdown causes the inhibition of this process. We then used bioinformatics tools and luciferase reporter assay to establish that dishevelled segment polarity protein 3 (DVL3), a key regulator of the Wnt/β-catenin signaling pathway, is a direct target of miR-204-5p. In addition, the overexpression of DVL3 led to an increase in β-catenin and cyclin D1 (CCND1) expression and, by contrast, the knockdown of DVL3 led to a decrease in the expression of β-catenin and CCND1. The knockdown of DVL3 significantly promoted adipogenesis. Finally, we demonstrated that the overexpression of miR-204-5p induced the downregulation of β-catenin and the canonical Wnt target gene, CCND1, in mature adipoctyes, while its knockdown led to their upregulation. Taken together, our data suggest that miR-204-5p regulates adipogenesis by controlling DVL3 expression and subsequently inhibiting the activation of the Wnt/β-catenin signaling pathway.

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“…A number of herbal products have been reported to exert beneficial effects against obesity and anti-inflammation in both animal and human subjects; nevertheless, few studies have systemically assessed the relationship between herbal medicine and gut flora in an obesity/endotoxemia model in detail [17]–[19].…”

Section: Introductionmentioning

confidence: 99%

Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota

et al. 2014

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Background and AimIncreasing evidence has indicated a close association of host-gut flora metabolic interaction with obesity. Flos Lonicera, a traditional herbal medicine, is used widely in eastern Asia for the treatment of various disorders. The aim of this study was to evaluate whether unfermented or fermented formulations of Flos Lonicera could exert a beneficial impact to combat obesity and related metabolic endotoxemia.MethodsObesity and metabolic endotoxemia were induced separately or together in rats through feeding a eight-week high fat diet either alone (HFD control group) or in combination with a single LPS stimulation (intraperitoneal injection, 0.75 mg/kg) (LPS control group). While, the mechanism of action of the Lonicera formulations was explored in vitro using RAW 264.7 and HCT 116 cell lines as models.ResultsIn cell-based studies, treatment with both unfermented Flos Lonicera (UFL) and fermented Flos Lonicera (FFL) formulations resulted in suppression of LPS-induced NO production and gene expression of vital proinflammatory cytokines (TNF-α, COX-2, and IL-6) in RAW 264.7 cells, reduced the gene expression of zonula occludens (ZO)-1 and claudin-1, and normalized trans epithelial electric resistance (TEER) and horseradish peroxidase (HRP) flux in LPS-treated HCT-116 cells. In an animal study, treatment of HFD as well as HFD+LPS groups with UFL or FFL resulted in a notable decrease in body and adipose tissue weights, ameliorated total cholesterol, HDL, triglyceride, aspartate transaminase and endotoxin levels in serum, reduced the urinary lactulose/mannitol ratio, and markedly alleviated lipid accumulation in liver. In addition, exposure of HFD as well as HFD+LPS groups with UFL or FFL resulted in significant alteration of the distribution of intestinal flora, especially affecting the population of Akkermansia spp. and ratio of Bacteroidetes and Firmicutes.ConclusionThis evidence collectively demonstrates that Flos Lonicera ameliorates obesity and related metabolic endotoxemia via regulating distribution of gut flora and gut permeability.

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Anti-obesity effects of Actinidia polygama extract in mice with high-fat diet-induced obesity

Cited by 16 publications

References 21 publications

Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice

Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice

miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/β-catenin signaling

Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota

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