Anti-obesity effect of phenylcoumarins from two Calophyllum spp in 3T3-L1 adipocytes

Thiagarajan, Siroshini K; Yong, Fui-Lu; Hemaroopini, Subramaniam; Jong, Vivien Yi Mian; Lim, C.-Y; Say, Yee‐How

doi:10.4314/tjpr.v16i3.9

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…The seed and shell parts of the E. tapos fruit were included in this investigation since they have been documented to contain flavonoids, tannin, alkaloids, oleic acid, linolenic acid, saturated and unsaturated fatty acids (6). Flavonoids are polyphenolic substances that are added to the human diet (7) and have been shown to help in obesity treatment (8). As a result, the goal of this study is to see if E. tapos fruit extracts may inhibit adipogenesis in 3T3-L1 cells.…”

Section: Introductionmentioning

confidence: 99%

Anti-Adipogenic Effect of Elateriospermum Tapos on 3t3-L1 Cells

Thiagarajan,

Perumal,

Bahari

et al. 2023

JUMMEC

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Obesity is defined as an excessive fat accumulation that causes metabolic syndrome such as diabetes, hypertension, and abnormal cholesterol levels. Adipocytes are a major part of the adipose tissue that grows the abnormal lipid droplets which leads to fat accumulation. In modern drugs, treating obesity without any side effects is challenging. Natural products such as plants and herbs are widely used to cure obesity. Natural products are safer because of their reduced toxicity and infrequent negative effects. This study focuses on examining the potential anti-adipogenesis effects of Elateriospermum tapos on 3T3-L1 cells. The extracts from both seed and shell of the fruit were extracted using hot, cold and ethanol extraction. The extracts were tested for the cytotoxicity on 3T3-L1 cells and on zebrafish embryo in vivo. Zebrafish study on heartbeat, heart rate and scoliosis formation, shell extract show less toxic compared to seed extract. This shell extracts showed a positive correlation determination of R2 = 0.96. Therefore, shell extracts were used to indicate the maximum non-toxic dose (MNTD) of each extraction. The lowest MNTD was observed in ethanol extract at 5.2 ± 0.01 µg/mL, hot extract at 7.6 ± 0.25 µg/mL and cold extract at 8.1 ± 0.31 µg/mL. Oil Red O staining to determine the amount of lipid accumulation showed a significant (P < 0.05) decrease of 70% when compared to adipocytes when treated with ethanol shell extract, and a significant (P < 0.05) decrease of 60% by hot shell extract. With the reduction in lipid accumulation, lipolysis was measured by accounting the amount of glycerol produced. The highest production of glycerol was significant when treated with hot shell extract at 42 mg/L, followed by cold extract at 38 mg/L. This is concluded that the hot shell extract has potential to curb obesity by reducing lipid accumulation through lipolysis at a maximum non-toxic dosage.

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Section: Introductionmentioning

confidence: 99%

Anti-Adipogenic Effect of Elateriospermum Tapos on 3t3-L1 Cells

Thiagarajan,

Perumal,

Bahari

et al. 2023

JUMMEC

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5,8-Dihydroxy-2,2-dimethyl-12-(3-methylbut-2-enyl)pyrano[3,2-b]xanthen-6-one (brasixanthone B)

Seruji¹,

Rosli²,

Jong³

et al. 2023

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The title compound (trivial name brasixanthone B), C23H22O5, isolated from Calophyllum gracilentum, is characterized by a xanthone skeleton of three fused six-membered rings plus an additional fused pyrano ring and one 3-methylbut-2-enyl side chain. The core xanthone moiety is almost planar, with a maximum deviation 0.057 (4) Å from the mean plane. In the molecule, an intramolecular O—H...O hydrogen bond forms an S(6) ring motif. The crystal structure features intermolecular O—H...O and C—H...O interactions.

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Anti-obesity effect of phenylcoumarins from two Calophyllum spp in 3T3-L1 adipocytes

Abstract: Purpose: To evaluate the anti-obesity effects of five compounds isolated from Calophyllum andersonnii and Calophyllum sclerophyllum, viz, friedelin (CP1), friedelinol (CP2), isodispar B (CP3),5,

Cited by 2 publications

References 21 publications

Anti-Adipogenic Effect of Elateriospermum Tapos on 3t3-L1 Cells

Anti-Adipogenic Effect of Elateriospermum Tapos on 3t3-L1 Cells

5,8-Dihydroxy-2,2-dimethyl-12-(3-methylbut-2-enyl)pyrano[3,2-b]xanthen-6-one (brasixanthone B)

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