Anti-obesity Effect ofDioscorea nipponicaMakino with Lipase-inhibitory Activity in Rodents

Abstract

In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

“…9) Biological activities of Dioscorea species against diverse metabolic disorders including diabetes and obesity have been already documented. 10,11) However, anti-obesity effect of certain extract derived from D. oppositifolia in animal model remains to be defined. Here, based on the previous in vitro results of D. oppositifolia, we investigated the direct effect of D. oppositifolia n-BuOH extract on obesity induced by highfat diet in animal model.…”

Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway

“…However, the restraining effects and involved mechanisms of DNE on cancer metastasis have not yet to be elucidated. DNE has reported antiobesity and antiinflammation effects in both in vivo and in vitro experimental models (Kwon et al 2003;Xiao et al 2010). To date, no studies describe the anticancer effects of DNE on neuroblastoma cancer Figure 3.…”

“…The rhizome of Dioscorea nipponica Makino has high saponin content and use in folk medicine as treatment for coronary heart disease, asthma, rheumatoid arthritis, and hyperlipidemia, as well as a source of hormonal sterol synthesis precursors (Yoshikawa et al 2007). Recently, studies revealed that Dioscorea plant extracts exert antiobesity effects (Kwon et al 2003;Xiao et al 2010). However, limited studies exist concerning the anticancer effects of D. nipponica.…”

The Study on Anti-obesity Effects of Mulberry Leaves Contained Herbal Mixture

“…상엽 (桑葉, Folium Mori)에 대한 연구로는 혈당강하 효과 14,15) 와 cholesterol 감소 및 동맥경화증, 고지혈증, 당뇨병 예방치료 효과 16) 가 있고, 산약 (山藥, Rhizoma Dioscoreae)에 대한 연구로는 항산화 작용 17,18) , 고혈당 19) 과 비만 20) 을 억제하는 작용과 지질대사 개선 Cells were incubated with MLHM at the indicated concentration (0.04-4mg/mL) for 24h and the growth rate was assessed by MTT assay. 1: control; 2: MLHM 0.04mg/ml; 3: MLHM 0.4mg/ml; 4: MLHM 4mg/ml All values are mean ± SD.…”