Anti-obesity activity of hen egg anti-lipase immunoglobulin yolk, a novel pancreatic lipase inhibitor

Abstract

BackgroundThere is completely no report about both hen egg anti-lipase immunoglobulin yolk (IgY) and its anti-obesity action. Thus, we tried to isolate and characterize a novel anti-lipase immunoglobulin from hen egg yolk. Moreover, we investigated whether hen egg yolk anti-lipase IgY inhibits pancreatic lipase activity in vitro, and examined its ability to prevent obesity in a murine high fat diet-induced obesity model.MethodsWe determined the inhibitory action of Anti-lipase IgY on lipase activity in vitro. We also focused our evaluation on the anti-obesity properties of Anti-lipase IgY in a murine high fat diet-induced obesity model.ResultsAnti-lipase IgY blocked porcine lipase activity with an IC50 of 0.49 μM. Supplementing the high fat diet with only 0.2% (w/w) of Anti-lipase IgY for 35 days significantly decreased the weights of intraperitoneal adipose tissues, epididymal, mesenteric, retroperitoneal and perirenal adipose tissues, and the amounts of hepatic total lipid, triglyceride, and cholesterol. This was accompanied by a significant increase in the fecal excretion of triglyceride in the absence of diarrhea. Furthermore, Anti-lipase IgY treatment restored body weight gain to levels similar to mice fed with Control IgY.ConclusionsThis study provides the first report of the development of anti-lipase IgY and the direct evidence that inhibition of pancreatic lipase using Anti-lipase IgY is an effective anti-obesity treatment due to the associated increase in fecal excretion of triglyceride.

Physiological Characteristics and Anti-obesity Effect of Lactobacillus plantarum Q180 Isolated from Feces

“…Several approaches to the prevention and treatment of obesity have been reported ( Birari et al ., 2007 ). Among these, both natural and synthetic pancreatic lipase inhibitors are effective in preventing obesity, which is likely to be due to their inhibition of intestinal lipid absorption ( Hirose et al ., 2013 ). After being incubated in MRS broth at 37℃ for 18 h, three kinds of strains having a lipase inhibitory activity of over 60% were selected from among the 188 strains by measuring their lipase inhibitory activity.…”

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

“…Later, mice with obesity induced by high fat diet were orally treated with the antibody, which was given concomitantly with food, and a reduction of adipose tissue and liver fat level was observed, as well as an increase of fecal excretion of triglycerides and their decrease in blood plasma. Anti-lipase IgY inhibited the hydrolysis of diet fat and reduced its intestinal absorption, showing anti-obesity activity [89].…”

Anti-obesity Effect of Yogurt Fermented by Lactobacillus plantarum Q180 in Diet-induced Obese Rats

“…Obesity is a serious medical condition in which excess body fat accumulates to the extent that it may have a negative effect on health, leading to reduced life expectancy and/or increased health problems ( Kopelman, 2000 ; Plessis et al ., 2010 ). Obesity causes various kinds of metabolic syndrome such as diabetes, insulin resistance, hypertension, arteriosclerosis, cardiovascular diseases, type-2 diabetes, certain cancers, and sleep apnea/sleep-disordered breathing ( Haslam and James, 2005 ; Hirose et al ., 2013 ).…”