Anti-nutritional factors and digestibility of protein in Cayocar brasiliense seeds

Abstract

The Brazilian Cerrado presents a wide variety of natural products, including the Pequi (Caryocar brasiliense). The important factor that determines the dietary and nutritional ideality of protein is degestitibility. This work aims to evaluate the protein digestibility of Pequi seeds and the presence of anti-nutritional factors. The protein Pequi almonds were extracted, toasted and untoasted. Evaluation digestibility was structurally characterized by SDS-PAGE 15%, which can be used to analyze simulated gastric fluid (SFG) and digestion in intestinal fluid (SFI) in the presence of pepsin and trypsin/chymotrypsin. The extract from Pequi almonds showed inhibitory activity and was not detected hemagglutination. The intensity of the bands according to 2S albumins, after heat treatment, did not decrease in comparison to the condition native, significantly. In the crisp crude extract, which was incubated under SGF and SIF, the intensity of the corresponding bands at 8 and 3 kDa was resistant to SGF and indigestible after 4 hours under SIF. The characterization of Pequi almonds demonstrated that the product of the crude extract has anti-nutritional factors, which were confirmed by evaluating digestibility.Keywords: Caryocar brasiliense; almonds; in vitro digestibility; 2S albumin; peptidase inhibitor; lectins.Practical Application: The study of anti-nutritional factors identification is very important because of its inactivation on food industry and it can also be used to avoid allergy due to them.

Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds

“…Therefore, the in vitro digestion simulation experiment of the Zihua snap bean lectin was studied. SDS–PAGE has been widely used in simulated gastric fluid (SGF) analysis and simulated intestinal fluid (SIF) analysis [45]. SDS–PAGE analyses of native lectin from the Zihua snap bean in SGF and SIF are shown in Figure 10.…”

Nutritional composition and biological activities (antioxidant and antifungal) of Sesbania virgata (Cav.) Pers. seeds

“…Hossain;Becker (2001) report the presence of these antinutritional factors in S. aculeata, S. rostrata and S. sesban seeds, but its contents are very low. Although, when isolated, these antinutritional factors have biological relevance, its absence in S. virgata seed is an interesting finding, because lectins and trypsin inhibitors generally do not degrade easily in the gastrointestinal tract and can be complexed with digestive enzymes, interfering in the nutrient absorption (SILVESTRINI et al, 2017). Additionally, the acceptability and use of legumes seeds have been affected by the presence of these antinutritional factors, which reduce the bioavailability nutrients (SHARAN et al, 2021).…”

Influence of Different Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

“…Previous studies showed that Burkina Faso cowpea cultivars are rich in mineral elements (Fe, Zn, Mg, Ca, Se, K, Na), phenolic compounds and have significant antioxidant and anti-lipid peroxidation activities [5,6]. Legumes also contain antinutritional factors like saponin, heamaglutinin, cyanogenic glycoside, phytic acid, tannin, trypsin and chymotrypsin inhibitors that may reduce the bioavailability of nutrients and also cause harmful effects to human health [7,8]. Storage organs of plant such as seeds, tubers and also leaves, flowers, and fruits contain different types of protease inhibitors (PIs) which is able to inhibit some enzymes like trypsin, pepsin and chymotrypsin [9,10,7,11].…”

“…Legumes also contain antinutritional factors like saponin, heamaglutinin, cyanogenic glycoside, phytic acid, tannin, trypsin and chymotrypsin inhibitors that may reduce the bioavailability of nutrients and also cause harmful effects to human health [7,8]. Storage organs of plant such as seeds, tubers and also leaves, flowers, and fruits contain different types of protease inhibitors (PIs) which is able to inhibit some enzymes like trypsin, pepsin and chymotrypsin [9,10,7,11]. PIs are small proteins or peptides that inhibit the catalytic action of proteases by forming stoichiometric and stable complexes with their proteolytic enzymes blocking or altering the active site [11][12][13].…”