Anti‐nonspecific adsorption segments‐assisted self‐driven surface imprinted fibers for efficient protein separation

Abstract

At present, the development of high-performance protein imprinted materials is still a research hotspot in the field of protein imprinting. Herein, anti-protein adsorption segment (CBMA)-assisted self-driven bovine serum albumin (BSA) surface imprinted fibers MTCFs@SIP@CBMA with high recognition selectivity are pioneered using the strategies of combining magnetic nanomaterial surface imprinting technique with amino-Michael addition. The special structure of the carrier MTCFs endows MTCFs@SIP@CBMA with magnetic performance and self-driven adsorption performance, which simplifies the separation process while improving the adsorption capacity and accelerating the adsorption rate. The adsorption capacity for BSA reached 390 ± 20 mg/g within 30 min. The introduction of CBMA segments on the surface after imprinting by amino-Michael addition makes its polymer chain length and position controllable. Under the strongest anti-nonspecific adsorption effect, MTCFs@SIP@CBMA exhibit excellent specific identification to BSA from mixed proteins. Additionally, MTCFs@SIP@CBMA show considerable reusability. Therefore, MTCFs@SIP@CBMA are expected to be applied in efficient separation of proteins in biological samples.

Magnetic Fe‐Ni bimetallic oxide shell nanorods containing anti‐protein polymer segments for protein purification

“…Considering the abundance of groups on the surface of proteins and their complex structures, it is not surprising that they will interact with the material and thus non-specific adsorption occurs during the recognition by MIPs. 31 Researchers have proposed to introduce "protein resistance" into the imprinting layer in order to reduce non-specific adsorption. 32,33 Inspired by the resistance to protein adsorption of anti-fouling materials such as polyethylene glycol (PEG) and amphiphilic polymer materials, it is possible to reduce the non-specific adsorption by introducing "protein resistance" in the imprinted layer.…”

“…Although the surface imprinting strategy can overcome the shortcomings of mass transfer, the addition of high specificity is required to promote its practical industrial application. Considering the abundance of groups on the surface of proteins and their complex structures, it is not surprising that they will interact with the material and thus non‐specific adsorption occurs during the recognition by MIPs 31 . Researchers have proposed to introduce “protein resistance” into the imprinting layer in order to reduce non‐specific adsorption 32,33 .…”

Fabrication of Metal Ion–BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation

“…On the other hand, using nanomaterials with a high surface area as carriers can provide more imprinting binding sites, which is conducive to protein enrichment and anchorage. Many nanomaterials including magnetic Fe 3 O 4 , tubular nanofibers (FTCFs), , graphene, and carbon nanotubes (CNTs) have been used as promising carriers to apply in various fields.…”

Fabrication of High-Recognition Self-Driven Protein Surface Imprinted Mesoporous N-Doped Carbon Fiber

“…In our previous works, surface imprinted tubular carbon nanofibers (SIPTCFs), surface imprinted magnetic tubular carbon nanofibers (SIPMTFs), and surface imprinted manganese dioxide-loaded tubular carbon fibers (FTCFs@MnO 2 @MIPs) with a self-driven property were developed by using tubular carbon nanofibers as the carrier. Self-driven imprinting refers to the process where target protein is spontaneously adsorbed on imprinting sites under its own conditions without external forces . To achieve self-driven performance, it is required that the carrier material contains a cavity inside the carrier material and porous tube walls for the protein to travel through.…”