Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

Abstract

Background: To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods: Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results: TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100-400 mg/ kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion: These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.

Novel 1,3,4-Oxadiazole Derivatives of Pyrrolo[3,4-d]pyridazinone Exert Antinociceptive Activity in the Tail-Flick and Formalin Test in Rodents and Reveal Reduced Gastrotoxicity

“…It has been demonstrated that ROS and RNS are able to decrease glutamate transporters activity and increase vanilloid and N -methyl-D-aspartate receptors activity [ 31 ]. Therefore, the antiradical properties of any substance may play an important role in the reduction of inflammatory nociception [ 32 ]. In our study, compounds 13b , but not 10b , at the medium and high doses decreased MPO level compared to the control group.…”

In silico profiling of analgesic and antihyperglycemic effects of ethanolic leaves extract of Amischotolype mollissima: Evidence from in vivo studies

“…In order to identify potential peripherally acting antinociceptive agents, this nociceptive model is quite efficient for dosages in which the analgesic and anti-inflammatory effects of drugs would be ineffective in other pain models ( Nock et al, 2022 ). When acetic acid is administered by intraperitoneal injection, peripheral nociception is induced upon direct activation of non-selective cationic channels or indirect release of different endogenous mediators namely, prostaglandins, cytokines, bradykinin, along with increased production of the enzymes lipoxygenase (LOX) and cyclooxygenase (COX) ( Alabi et al, 2019 ), thereby causing the stimulation of nociceptive neurons sensitive to the non‐steroidal anti-inflammatory drugs ( Prabhu et al, 2011 ). Therefore, this test can be utilized to look into novel mild analgesic non-steroidal anti-inflammatory drugs.…”

Evaluation of Pharmacological Potentialities of Polar and Nonpolar Fractions of Justicia aurea Extract

“…This nociceptive model is extremely successful for dosages where the analgesic and anti-inflammatory effects of medication would be ineffective in other pain models 16 . Peripheral nociception is activated through the direct induction of non-selective cationic channels or the indirect secretion of various endogenous mediators, such as prostaglandins, cytokines, and bradykinin, as well as elevated production of lipoxygenase and cyclooxygenase enzymes resulting in the activation of neurons sensitive to nonsteroidal anti-inflammatory drugs (NSAIDs) 17,18 . Therefore, this test could be used to investigate the antinociceptive activity of novel NSAIDs.…”