Anti-Neospora caninum and anti-Sarcocystis spp. specific antibodies cross-react with Besnoitia besnoiti and influence the serological diagnosis of bovine besnoitiosis

García-Lunar, Paula; Moré, Gastón; Campero, Lucía María; Ortega‐Mora, Luis Miguel; Álvarez‐García, Gema

doi:10.1016/j.vetpar.2015.09.011

Cited by 29 publications

(13 citation statements)

References 36 publications

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“…and Sarcocystis spp. [16]. In this study, six equids resulted positive to Besnoitia spp., with an overall seroprevalence of 2.1% (0.7% in horses and 22.2% in donkeys).…”

Section: Accepted Manuscriptmentioning

confidence: 48%

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

Villa

Gazzonis

Álvarez‐García

et al. 2018

Parasitology International

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Among Apicomplexa protozoa infecting equids, Besnoitia spp., Toxoplasma gondii and Neospora spp. represent important issues from a sanitary and zootechnical viewpoint. However, only scarce epidemiological data are available on the spread of the infections in horses and donkeys in Europe. Therefore, a serosurvey was planned to estimate the prevalence of these Sarcocystidae species in Italian equids. Serum samples from 268 horses and 18 donkeys raised in Italy were collected and serologically analyzed to detect anti-Besnoitia spp., anti-T. gondii and anti-Neospora spp. antibodies: an approach based on an initial screening by in-house ELISA followed by a confirmatory WB was used. Two horses (0.7%) and four donkeys (22.2%), showed antibodies anti-Besnoitia spp. Ten horses (3.7%) resulted positive to T. gondii and one of these (0.4%) was seropositive also to Neospora spp. This is the first detection of anti-Besnoitia spp. specific antibodies in Italian horses and donkeys. The study confirmed the circulation of Besnoitia spp. among equids in Europe. Low prevalence of T. gondii and Neospora spp. in horses raised in Italy was reported. Nevertheless, it is noteworthy to consider that consumption of horse meat could represent a source for human toxoplasmosis.

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“…and Sarcocystis spp. [16]. In this study, six equids resulted positive to Besnoitia spp., with an overall seroprevalence of 2.1% (0.7% in horses and 22.2% in donkeys).…”

Section: Accepted Manuscriptmentioning

confidence: 48%

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

Villa

Gazzonis

Álvarez‐García

et al. 2018

Parasitology International

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“…Also immunological cross reaction between Besnoitia besnoiti antigens and anti Neospora caninum has been shown [17].…”

Section: Discussionmentioning

confidence: 97%

A Nonglycosylated 27 KDa Molecule as Common Antigen between Human Breast Cancer and <i>Echinococcus granulosus</i> Hydatid Cyst Wall

Sharafi¹,

Rafiei²,

Hadipour³

et al. 2016

ABCR

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Background: Hydatid cyst, which has anti-cancer activities, is outwardly covered with the cyst wall. It is in close contact with the host tissues and its molecules presented to the immune system. In this work immunological reaction of the sera of breast cancer patients with the hydatid cyst wall antigens has been investigated. Method: For this purpose, sera of patients with breast cancer, hydatid cyst and normal human sera were collected and their reaction with hydatid cyst wall antigens was tested using western immunoblotting technique. Results: All sera of patients with breast cancer, hydatid cyst and also human normal sera reacted with a band in western immunoblotting. However, sera of patients with breast cancer showed reaction with a 27 KDa band. Results of this work also revealed that this band was not glycosylated and may express only in some stages of breast cancer development. Conclusion: Sera of patients with breast cancer cross reacted with a nonglycosylated antigen of hydatid cyst wall. However, more work is recommended to find if this band involves in anticancer activity of the hydatid cyst.

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“…Two animals were inoculated intraperitoneally with 2x10 5 bradyzoites of Sarcocystis spp. obtained from cysts of a naturally infected bovine heart and purified by previously described by García-Lunar et al (2015). Similarly, two animals were inoculated intraperitoneally with 2x10 5 tachyzoites of N. caninum and two animals with T. gondii.…”

Section: Methodsmentioning

confidence: 99%

Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle

et al. 2020

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Serological techniques can detect antibodies against Sarcocystis spp., Neospora caninum and Toxoplasma gondii antigens in single or mixed infections. Immunofluorescent antibody tests (IFAT) is considered the gold standard technique for Sarcocystosis diagnostic in cattle serum and a positive IFAT result reflects Sarcocystis spp. infection. Therefore, the aims of the present study were to compare IFAT and Dot-blot for sarcocystosis diagnostic in experimentally infected mice and to investigate serological cross-reactions with N. caninum and T. gondii in these methods. Mice (Mus musculus) were inoculated intraperitoneally with bradizoites of Sarcocystis spp. or tachyzoites of N. caninum or T. gondii. Serum samples were obtained and analyzed by IFAT and Dot-blot for the three protozoa. Serum from N. caninum and T. gondii experimentally infected mice were tested by IFAT and reacted only to N. caninum or T. gondii antigens, respectively. Specific antibodies against Sarcocystis spp. were present in all animals experimentally infected with this protozoan, with IFAT titers from 10 to 800. Serum samples from mice experimentally infected with Sarcocystis spp., N. caninum and T. gondii and tested by Dot-blot demonstrated no cross reaction between protozoa. A Dot-blot using Sarcocystis spp. antigen appears to be a good alternative to IFAT in the serological diagnosis of Sarcocystosis.

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Anti-Neospora caninum and anti-Sarcocystis spp. specific antibodies cross-react with Besnoitia besnoiti and influence the serological diagnosis of bovine besnoitiosis

Cited by 29 publications

References 36 publications

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

A Nonglycosylated 27 KDa Molecule as Common Antigen between Human Breast Cancer and <i>Echinococcus granulosus</i> Hydatid Cyst Wall

Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle

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Anti-Neospora caninum and anti-Sarcocystis spp. specific antibodies cross-react with Besnoitia besnoiti and influence the serological diagnosis of bovine besnoitiosis

Cited by 29 publications

References 36 publications

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

First detection of anti- Besnoitia spp. specific antibodies in horses and donkeys in Italy

A Nonglycosylated 27 KDa Molecule as Common Antigen between Human Breast Cancer and &lt;i&gt;Echinococcus granulosus&lt;/i&gt; Hydatid Cyst Wall

Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle

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A Nonglycosylated 27 KDa Molecule as Common Antigen between Human Breast Cancer and <i>Echinococcus granulosus</i> Hydatid Cyst Wall