Anti-neoplastic effect of epigallocatechin gallate on breast cancer cells through glucose metabolism

Abstract

Breast cancer (BC) is the primary cause of women cancer death, which could be prevented by EGCG that has been recently shown several health properties included anti-cancer, however the mechanism underpinning still poorly understood. In this study, several biological activities of both MCF7 and MDA-MB-231 cells were evaluated in response to EGCG. Cell viability and the role of Akt and AMPK inhibitor molecules, and sodium pyruvate on this viability, apoptosis, metastasis, and interestingly regulation of glucose metabolism were assessed. EGCG promoted cytotoxicity in both BC cell lines after 24h but not less. Co-incubated cells with Akt and AMPK inhibitors alongside EGCG significantly caused more reduction in cell viability compared to the effect of EGCG alone with maximum effect referred to Akt inhibitor. While supplemented sodium pyruvate significantly restored the decreases in cell viability. Remarkably, EGCG induced apoptosis through increased caspase 3/7 activation associated with upregulated Bax gene, in addition to anti-metastatic effect through decreasing cellular migration. Importantly, lactate production was sharply reduced after 6h (no alteration of viable cells) and 24h (decreased viable cells) concomitant with significant blocked glucose uptake in response to EGCG. In conclusion, EGCG could be a potential anti-migration, the anti-cancerous therapeutic agent through targeting cancer cells glucose metabolism.

EGCG inhibits the inflammation and senescence inducing properties of MDA-MB-231 triple-negative breast cancer (TNBC) cells-derived extracellular vesicles in human adipose-derived mesenchymal stem cells

“…To characterize the EVs release, serum-starved TNBC-derived MDA-MB-231 cells were cultured in the presence or absence of 30 µM EGCG for 48 h. A concentration documented to not alter MDA-MB-231 cell viability [ 45 – 47 ]. Conditioned media was next collected, and EVs were isolated as described in the Methods section.…”

Níveis De Glicose Sanguínea Em Camundongos C57bl/6 Submetidos Ao Exercício Na Escada Com E Sem Estimulação De Choque

“…Ademais, pesquisas comprovaram que esse elemento obstaculiza o desenvolvimento de células TNBC por meio da inoperância do caminho de sinalização de β-catenin 43 . Igualmente, mostra ações anti-migração e pró-apoptose por meio do estímulo de caspases -3/7 e normatização de Bax 44 .…”

Antioxidants for the Treatment of Breast Cancer: Are We There Yet?

“…Furthermore, studies have demonstrated that EGCG inhibits the growth of TNBC cells through the inactivation of the β-catenin-signaling pathway [ 153 , 154 ]. Remarkably, this antioxidant exhibits anti-migration and pro-apoptosis activities through the activation of caspases 3/7 and upregulation of Bax [ 155 ]. Moreover, the combination with curcumin and α-tocopheryl succinate into polystyrene–polysoyaoil–diethanolamine nanoparticles demonstrated to inhibit tumor growth and reduce toxicity when compared with single-drug chemotherapy [ 156 ].…”