Anti-neoplastic and immunostimulatory effects of low-dose X-ray fractions in mice

Nowosielska, Ewa M.; Cheda, Aneta; Wrembel-Wargocka, Jolanta; Janiak, Marek K.

doi:10.3109/09553002.2010.519422

Cited by 20 publications

(22 citation statements)

References 53 publications

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“…While whole body irradiation (WBI) with a dose of 2 Gy resulted in increased NK cell apoptosis, low-dose radiation (0.01-0.1 Gy) decreased NK cell apoptosis [45]. Interestingly, a 5 day/ week radiation schedule with fractions up to 0.1 Gy was shown to increase cytotoxicity of NK cell-enriched splenocytes and to reduce the number of pulmonary tumor colonies [46]. Mechanistically, ionizing radiation seems to enhance expansion and cytotoxicity of NK cells at least partially by the P38-MAPK pathway [47].…”

Section: Activation Of Nk Cells Is Modulated By Ionizing Radiationmentioning

confidence: 97%

Modulation of inflammation by low and high doses of ionizing radiation: Implications for benign and malign diseases

et al. 2015

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Section: Activation Of Nk Cells Is Modulated By Ionizing Radiationmentioning

confidence: 97%

Modulation of inflammation by low and high doses of ionizing radiation: Implications for benign and malign diseases

et al. 2015

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“…Authors noted that the mechanism of such antitumor efficacy might be related to the activation of an immune system, i.e. the stimulation of cytotoxic activities of peritoneal macrophages and natural killer cells, which resulted in a decrease of pulmonary tumor colonies [8,[15][16][17].…”

Section: Resultsmentioning

confidence: 99%

Macrophage and tumor cell responses to repetitive pulsed X-ray radiation

Buldakov¹,

Tretyakova²,

Ryabov

et al. 2017

J. Phys.: Conf. Ser.

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On the question of use of dosimetric devices in the registration of pulsed X-ray radiation L V Vladimirov, B V Artemiev, S E Okhrimenko et al. Abstract. To study a response of tumor cells and macrophages to the repetitive pulsed lowdose X-ray radiation. Methods. Tumor growth and lung metastasis of mice with an injected Lewis lung carcinoma were analysed, using C57Bl6. Monocytes were isolated from a human blood, using CD14+ magnetic beads. IL6, IL1-betta, and TNF-alpha were determined by ELISA. For macrophage phenotyping, a confocal microscopy was applied. "Sinus-150" was used for the generation of pulsed X-ray radiation (the absorbed dose was below 0.1 Gy, the pulse repetition frequency was 10 pulse/sec). The irradiation of mice by 0.1 Gy pulsed X-rays significantly inhibited the growth of primary tumor and reduced the number of metastatic colonies in the lung. Furthermore, the changes in macrophage phenotype and cytokine secretion were observed after repetitive pulsed X-ray radiation. Conclusion. Macrophages and tumor cells had a different response to a low-dose pulsed X-ray radiation. An activation of the immune system through changes of a macrophage phenotype can result in a significant antitumor effect of the low-dose repetitive pulsed X-ray radiation.

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“…3)—a molecule responsible for cytotoxic activity of these cells (Nowosielska et al 2011). Moreover, the same total absorbed doses of the HTO-derived radiation enhanced cytolytic activity of NK splenocytes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of internal contamination with tritiated water on the neoplastic colonies in the lungs, innate anti-tumour reactions, cytokine profile, and haematopoietic system in radioresistant and radiosensitive mice

Nowosielska

Cheda

Zdanowski

et al. 2018

Radiat Environ Biophys

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Tritium is a potentially significant source of internal radiation exposure which, at high levels, can be carcinogenic. We evaluated whether single intraperitoneal injection of BALB/c and C57BL/6 mice with tritiated water (HTO) leading to exposure to low (0.01 or 0.1 Gy) and intermediate (1.0 Gy) cumulative whole-body doses of β radiation is immunosuppressive, as judged by enhancement of artificial tumour metastases, functioning of NK lymphocytes and macrophages, circulating cytokine’s levels, and numbers of bone marrow, spleen, and peripheral blood cells. We demonstrate that internal contamination of radiosensitive BALB/c and radioresistant C57BL/6 mice with HTO at all the absorbed doses tested did not affect the development of neoplastic colonies in the lungs caused by intravenous injection of syngeneic cancer cells. However, internal exposure of BALB/c and C57BL/6 mice to 0.1 and 0.01 Gy of β radiation, respectively, up-regulated cytotoxic activity of and IFN-γ synthesis in NK lymphocytes and boosted macrophage secretion of nitric oxide. Internal contamination with HTO did not affect the serum levels of pro- (IL-1β, IL-2, IL-6, TNF-α,) and anti-inflammatory (IL-1Ra, IL-4, IL-10) cytokines. In addition, exposure of mice of both strains to low and intermediate doses from the tritium-emitted β-particles did not result in any significant changes in the numbers of bone marrow, spleen, and peripheral blood cells. Overall, our data indicate that internal tritium contamination of both radiosensitive and radioresistant mice leading to low and intermediate absorbed β-radiation doses is not immunosuppressive but may enhance some but not all components of anticancer immunity.

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Anti-neoplastic and immunostimulatory effects of low-dose X-ray fractions in mice

Abstract: Suppression of the growth of pulmonary tumour colonies by irradiations of mice with low-dose fractions of X-rays may result from stimulation of anti-tumour reactions mediated by NK cells and/or cytotoxic macrophages.

Cited by 20 publications

References 53 publications

Modulation of inflammation by low and high doses of ionizing radiation: Implications for benign and malign diseases

Modulation of inflammation by low and high doses of ionizing radiation: Implications for benign and malign diseases

Macrophage and tumor cell responses to repetitive pulsed X-ray radiation

Effect of internal contamination with tritiated water on the neoplastic colonies in the lungs, innate anti-tumour reactions, cytokine profile, and haematopoietic system in radioresistant and radiosensitive mice

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